Effect of vitamin $D_3$ and/or zeolite supplementation to laying hen rations added microbial phytase on some blood indices 2. total cholesterol, 1,25-dihydroxycholecalciferol and oestradiol-17β levels

Çalışmanın amacı yumurta tavuklarında fitaz enzimi varlığında yeme vitamin D3 ve/veya zeolit ilavesinin serum total kolesterol, 1,25dihidroksikolekalsiferol (1,25-(OH)2$D_3$) ve östradiol-17β düzeyleri üzerine etkisini incelemektir. 28 haftalık 60 adet yumurta tavuğu 4 eşit gruba ayrılmıştır. Tavuklar kontrol rasyonu (300 fitaz ünitesi (FTU) fitaz/kg), deneme 1 rasyonu (300 FTU fitaz + 400 IU D3 vitamini), deneme 2 rasyonu (300 FTU fitaz + 400 IU D3 vitamini + %2 zeolit) ve deneme 3 rasyonu (300 FTU fitaz + %2 zeolit) ile beslenmişlerdir. Serum total kolesterol düzeyleri 12. hafta hariç, gruplar arasında anlamlı ölçüde farklı bulunmamıştır. 12. haftada düzeyler fitaz ve zeolit ilave edilen grupta fitaz ve D3 vitamini ilave edilen gruptan anlamlı ölçüde daha yüksek bulunmuştur (P

Mikrobiyal fitaz ilaveli yumurta tavuğu rasyonlarına $D_3$ vitamini ve/veya zeolit eklenmesinin bazı kan parametreleri üzerine etkisi 2. total kolesterol, 1,25- dihidroksikolekalsiferol ve östradiol-17β düzeyleri

The aim of this study was to examine the effect of vitamin D3 and/or zeolite supplementation in the presence of phytase enzyme on serum total cholesterol, 1,25-dihydroxycholecalciferol (1,25-(OH)2D3) and oestradiol-17&#946; levels in laying hens. A total of 60 laying hens, 28-wkold were separated to 4 equal groups. The hens were fed control diet (300 phytase units (FTU) phytase per kilogram), experimental 1 diet (300 FTU phytase + 400 IU vitamin D3), experimental 2 diet (300 FTU phytase + 400 IU vitamin D3 + 2% zeolite) and experimental 3 diet (300 FTU phytase + 2% zeolite). Serum total cholesterol levels were not statistically different between groups except for week 12. On week 12, these levels were significantly higher in the phytase and zeolite added group than in the phytase and vitamin D3 added group (P<0.05). Serum 1,25-(OH)2D3 levels were higher in the only phytase added group than in the other groups on week 16 and lower in the phytase and zeolite added group than in the other groups on week 8 (P<0.05). Serum oestradiol-17&#946; levels were higher in the phytase and zeolite added group than in the other groups on weeks 4 and 12, and lower in the phytase, vitamin D3 and zeolite added group than in the other groups on week 12 (P<0.05). Consequently, serum total cholesterol levels were not affected by different feeding regimes, phytase enzyme added to ration increased serum 1,25-(OH)2D3 levels, and phytase enzyme and vitamin D3 supplementation increased serum oestradiol-17&#946; levels..

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  • 1. Rama Rao SV, Ravindra Reddy V, Ramasubba Reddy V: Enhancement of phytate phosphorus availability in the diets of commercial broilers and layers. Anim Feed Sci Tech, 79, 211-222, 1999.
  • 2.Cromwell GL, Coffey RD, Parker GR, Monegue HJ, Randolph JH: Efficacy of a recombinant-derived phytase in improving the bioavailability of phosphorus in corn-soybean meal diets for pigs. J Anim Sci, 73, 2000-2008, 1995.
  • 3.Mroz Z, Jongbloed AW, Kemme PA: Apparent digestibility and retention of nutrients bound to phytate complexes as influenced by microbial phytase and feeding regimen in pigs. J Anim Sci, 72, 126-132, 1994.
  • 4.Nelson TS, Shieh TR, Wodzinski RJ, Ware JH: Effect of supplemental phytase on the utilization of phytate phosphorus by chicks. J Nutr, 101, 1289-1294, 1971.
  • 5.Simons PCM, Versteegh HAJ, Jongbloed AW, Kemme PA, Slump P, Bos KD, Wolters MGE, Beudeker RF, Vershoor GJ: Improvement of phosphorus availability by microbial phytase in broilers and pigs. Br J Nutr, 64, 524-540, 1990.
  • 6.Ballam GC, Nelson TS, Kirby LK: Effect of fiber and phytate source and of calcium and phosphorus level on phytate hydrolysis in the chick. Poult Sci, 63, 333-338, 1984.
  • 7.Edwards HM: Effect of dietary calcium, phosphorus, chloride and zeolite on the development of tibial dyschondroplasia. Poult Sci, 67, 1436-1446, 1988.
  • 8.Elliot MA, Edwards HM: Some effects of dietary aluminum and silicon on broiler chickens. Poult Sci, 70, 1390-1402, 1991.
  • 9.Roland DA: Further studies of effects of sodium aluminosilicate on egg shell quality. Poult Sci, 67, 577-584, 1988.
  • 10.Ingram DR, Kling CE, Laurent SM: Influence of ethacalTM feed component on production parameters of white leghorn hens during high ambient temperature. Nutr Rep Int, 37, 811818, 1988.
  • 11.Roland DA, Orban JI: Influence of various levels of dietary phosphorus and sodium chloride on the hen’s response to sodium aluminosilicate. Poult Sci, 68 (1): 124, 1989.
  • 12.DeLuca HF: Vitamin D endocrine system. Adv Clin Chem, 19, 125-174, 1977.
  • 13.Wasserman RH, Taylor AN: Vitamin D-dependent calcium-binding protein. Response to some physiological and nutritional variables. J Biol Chem, 243, 3987-3993, 1968.
  • 14.Parfitt AM, Mathews CH, Brommage R, Jarnagin K, DeLuca HF: Calcitriol but no other metabolite of vitamin D is essential for normal bone growth and development in the rat. J Clin Invest, 73, 576-586, 1984.
  • 15.Sedrani SH: Changes in serum levels of 1,25-dihydroxyvitamin D3, calcium and phosphorus with age and vitamin D status in chickens. Br J Nutr, 52, 329-334, 1984.
  • 16.Mrljak V, GrabarevićŽ, Džaja P, BarićRafaj R, PerićJ, ArtukovićB, Bidin Z, KišS, Stavljenic Rukavina A, Šerman V: Effect of different daily doses of gizzerosine on the serum 1,25-dihydroxycholecalciferol concentration in laying hens. Br Poult Sci, 40, 523-528, 1999.
  • 17.National Research Council: Nutrient Requirements of Poultry. National Academy of Science, Washington DC, USA, 1994.
  • 18.Stampfer R, Zucker H: Untersuchungen zur Beziehung zwischen ß-Carotin im Blut und in der Milch bei Kühen. Z Tierphysiol Tierer, 49, 140-147, 1983.
  • 19.Ergün G, Aktaş S: ANOVA modellerinde kareler toplamı yöntemlerinin karşılaştırılması. Kafkas Univ Vet Fak Derg, 15, 481-484, 2009.
  • 20.Maenz DD, Classen HL: Phytase activity in the small intestinal brush border membrane of the chicken. Poult Sci, 77, 557-563, 1998.
  • 21.Yu B, Jan YC, Chung TK, Lee TT, Chiou PWS: Exogenous phytase activity in the gastrointestinal tract of broiler chickens. Anim Feed Sci Tech, 117, 295-303, 2004.
  • 22.Kornegay ET, Denbow DM, Yi Z, Ravindran V: Response of broilers to graded levels of microbial phytase added to maize-soybean meal-based diets containing three levels of non-phytate phosphorus. Br J Nutr, 75, 839-852, 1996.
  • 23.Sohail SS, Roland DA Sr: Influence of supplemental phytase on performance of broilers four to 6 weeks of age. Poult Sci, 78, 550-555, 1999.
  • 24.Lipstein B, Hurwitz S: The effect of aluminum on the phosphorus availability in algae-containing diets. Poult Sci, 61, 951-954, 1982.
  • 25.Raisz LG, Trummel GL, Holick MF, DeLuca HF: 1,25dihydroxyvitamin: A potent stimulator of bone resorption in tissue culture. Science, 175, 768-769, 1972.
  • 26.Endo H, Kiyoki M, Kawashima K, Naruchi T, Hashimoto Y: Vitamin D3 metabolites and PTH synergistically stimulate bone formation of chick embryonic femur in vitro. Nature, 286, 262-264, 1980.
  • 27. Kato A, Seo EG, Einhorn TA, Bishop JE, Norman AW: Studies on 24R, 25- dihydroxyvitamin D3: Evidence for a nonnuclear membrane receptor in the chick tibial fracture-healing callus. Bone, 23, 141-146, 1998.
  • 28.Ruschkowski SR, Hart LE: Ionic and endocrine characteristics of reproductive failure in calcium-deficient and vitamin D-deficient laying hens. Poult Sci, 71, 1722-1732, 1992.
  • 29.Frost TJ, Roland DA Sr, Marple DN: Effects of various dietary phosphorus levels on plasma 1,25-(OH)2D3, ionized and total calcium, and phosphorus in commercial leghorns. Poult Sci, 70, 1564-1570, 1991.
  • 30.Edwards HM Jr: Dietary 1,25-dihydroxycholecalciferol supplementation increases natural phytate phosphorus utilization in chickens. J Nutr, 123, 567-577, 1993.
  • 31.Tanaka Y, DeLuca HF: The control of 25-hydroxyvitamin D metabolism by inorganic phosphorus. Arch Biochem Biophys, 154, 566-572, 1973.
  • 32.Frost TJ, Roland DA Sr, Barnes DG, Laurent SM: The effect of sodium zeolite A and cholecalciferol on plasma levels of 1,25-dihydroxycholecalciferol, calcium, and phosphorus in commercial leghorns. Poult Sci, 71, 886-893, 1992.
  • 33.Oguz H, Kececi T, Birdane YO, Onder F, Kurtoglu V: Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis. Res Vet Sci, 69, 89-93, 2000.
  • 34.Dwyer MR, Kubena LF, Harvey RB, Mayura K, Sarr AB, Buckley S, Bailey RH, Phillips TD: Effects of inorganic adsorbents and cyclopiazonic acid in broiler chickens. Poult Sci, 76, 1141-1149, 1997.
  • 35.Peeples ED, Branton SL, Burnham MR, Whitmarsh SK, Gerard PD: Effects of supplemental dietary phytase and 25hydroxycholecalciferol on the blood characteristics of commercial layers inoculated before or at the onset of lay with the F-strain of Mycoplasma gallisepticum. Poult Sci, 86, 768-774, 2007.
Kafkas Üniversitesi Veteriner Fakültesi Dergisi-Cover
  • ISSN: 1300-6045
  • Yayın Aralığı: Yılda 6 Sayı
  • Başlangıç: 1995
  • Yayıncı: Kafkas Üniv. Veteriner Fak.
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