BİLAL AKYÜZ, Okan ERTUĞRUL, ÖZGECAN KORKMAZ AĞAOĞLU

Detection of bovine leukocyte adhesion deficiency (BLAD) allele in Holstein cows reared in Kayseri vicinity

Bu çalışmanın amacı Kayseri civarında yetiştirilen Holştayn ineklerde sığır lökosit bağlanma yetmezliği (BLAD) allelinin varlığının araştırılmasıdır. Çalışmada 136 Holştayn inekten kan örneği toplanmıştır. Elde edilen PCR ürünlerinde mutasyon bölgesinin belirlenmesi için PCR ürünleri TaqI endonükleaz enzimi ile kesilmiştir. Çalışma sonunda incelenen 136 Holştayn ineğin üçünün BLAD taşıyıcısı oldukları belirlenmiştir. İncelenen 136 baş Holştayn inekte BLAD allelinin frekansı %2.2 olarak hesaplanmıştır.

Anahtar Kelimeler:

süt sığırı, süt ineği, saptama, teşhis, genetik kusurlar, mutant, Alacalı Sığır, Türkiye, alleller, sığır, sığır hastalıkları, inek

Kayseri civarında yetiştirilen Holştayn ineklerde sığır lökosit bağlanma yetmezliği (BLAD) allelinin belirlenmesi

The purpose of this work was to study whether the bovine leukocyte adhesion deficiency (BLAD) allele was present in the Holstein cows reared in Kayseri vicinity. Blood samples were obtained from 136 Holstein cows. In order to determine the area of mutation in PCR products, the PCR products were digested with TaqI endonuclease enzyme. It was found that three of the 136 Holstein cows were BLAD carriers. The mutant BLAD allele frequency in the 136 Holstein cows was calculated as 2.2%..

Keywords:

dairy cattle, dairy cows, detection, diagnosis, genetic disorders, mutants, Holstein-Friesian, Turkey, alleles, cattle, cattle diseases, cows,

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