Ali BİLGİLİ, Asım KART, Yılmaz GIGREMIS, BAŞAK HANEDAN, DİNÇER ERDAĞ

A comparative study on the effects of eprinomectin and ıvermectin on plasma antioxidant level and lipid peroxidation in cows

Avermektinlerden İvermektin ve Eprinomektin geniş spekturumlu endektositlerdir. Bu ilaçlar tedavi dozlarında ciddi yan etkileri olmayan ilaçlar olarak kabul edilir. Fakat avermektinler yüksek dozlarda hayvanlarda ve sağaltım dozunda insanlarda sinir sistemi ve genel semptomlarla karakterize bazı yan etkileri vardır. Bu çalışmada İvermektin ve Eprinomektin’in sağımdaki ineklerde plazma malondialdehid (MDA) ve glutatyon (GSH) seviyelerine etkisi araştUrUlmUştUr. 10 adet Holştayn ırkı süt veren inek 2 gruba ayrıldı. Grup 1 ve grup 2 ye sırasıyla tek doz olarak dökme tarzında İvermektin (0.5 mg/kg vücut ağırlığı) ve Eprinomektin (0.5 mg/kg vücut ağUrlUğU) uygulandU. İlaç uygulamasından önce kan örnekleri 0. saatte kontrol uygulamasU olarak vena jugularis’ten alındı. Kan örnekleri ilaç uygulamasını takiben belirli zaman aralıklarında (1, 4, 8, 24 ve 36. saatlerde ve 2, 3, 4, 5, 6, 8, 10, 12, 15, 20, ve 25. günlerde) yine jugular ven’den toplandı. Eprinomektin ve İvermektin GSH seviyesinde geçici bir düşmeye ve takiben yine geçici bir artışa sebep oldu (P0.05). Sonuç olarak, Eprinomektin ve İvermektinin plazma GSH seviyesinde geçici bir düşmeye sebeb olarak antioksidan kapasitede bir düşüşe neden olabileceği fakat bu düşüşün lipid peroksidasyonuna yol açmayacak derecede olduğu sonucuna varıldı.

Anahtar Kelimeler:

eprinomektin, ivermektin, antihelmintikler, glutatyon, kan plazması, yan etkiler, antioksidanlar, lipit peroksidasyonu, inek

İneklerde eprinomektin ve ivermektin'in plazma antioksidan ve lipid peroksidasyonu üzerine etkisinin karşılaştırmalı araştırılması

Avermectins including Ivermectin and Eprinomectin are broad spectrum endectocides. These drugs are considered to have no serious adverse effects at therapeutic range. However, use of avermectins in overdose in some animal species and at the therapeutic range in humans could result in some adverse effects including mainly nervous system effects and some general symptoms. This study was aimed to investigate effects of Eprinomectin and Ivermectin on plasma malondialdehyde (MDA) and glutathione (GSH) levels in lactating cow. Ten Holstein breed lactating dairy cows were divided into 2 groups. Before the application of drugs, blood samples were collected from the jugular vein in each group at 0 hour as control, and then Group 1 and 2 received a single pour-on application of Ivermectin (0.5 mg/kg body weight) and Eprinomectin (0.5 mg/kg body weight), respectively. Blood samples were then collected again from the jugular vein at timed-intervals (at 1, 4, 8, 24 and 36 hours and on 2, 3, 4, 5, 6, 8, 10, 12, 15, 20 and 25th days) to measure plasma MDA and GSH concentrations. Eprinomectin and Ivermectin treatment caused a transient decrease in GSH levels which is followed by transient increase (P<0.05). However, no difference was observed in MDA levels at all sampling points following the Eprinomectin and Ivermectin treatment compared to control samples (P>0.05). It was concluded that Eprinomectin and Ivermectin cause decreased GSH concentrations without alterations in MDA level leading to a decrease in the defense mechanism against oxidative stress although the decrease is not enough to cause lipid peroxidation.

Keywords:

eprinomectin, ivermectin, anthelmintics, glutathione, blood plasma, adverse effects, antioxidants, lipid peroxidation, cows,

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