DİLEK KESKİN, OKAN ATAY, ŞÜKRÜ KIRKAN, ÖZDAL GÖKDAL, Serten TEKBIYIK, Osman KAYA, VADULLAH EREN

Detection of Brucella melitensis in milk of Hair Goat (Capra hircus) by polymerase chain reaction (PCR)

Bu çalışmanın amacı, Aydın’ın Çine ilçesindeki KUl Keçilerinde B. melitensis’in yaygınlığını araştırmaktır. Türkiye’de B. melitensis insan ve hayvan sağlığı yönünden önemli bir sorundur. Bu çalışmada Kavşit, Mutaflar, Tatarmemişler ve Elderesi köylerinden 10 sürü ve116 keçiden 222 süt örneği toplanmıştır. Örnek alınan keçilerin yaşları 2-7 arasındadır ve brusellozise karşı aşılama yapılmamıştır. Bu araştırmada IS711, B. abortus ve B. melitensis için spesifik oligonükleotid primerleri kullanılmıştır. Süt örneklerinden DNA (Deoksiribonükleikasit) ekstraksiyonu yapıldıktan sonra, B. melitensis’i saptamak için PZR kullanılmıştır. 50 KUl Keçisi süt örneği (%22.5) B.melitensis için spesifik olan PZR 731 bp band aralığında pozitif sonuç vermiştir.

Anahtar Kelimeler:

Aydın, Brucella melitensis, DNA, brusellozis, hastalık yaygınlığı, süt, polimeraz zincir reaksiyonu, halk sağlığı, kıl keçisi

Kıl Keçisi (Capra hircus) sütlerinden polimeraz zincir reaksiyonu (PZR) ile Brucella melitensis saptanması

The aim of this study was to investigate to B. melitensis in Hair Goat in the Çine district of AydUn province. B. melitensis, is a significant problem for public and animal health in Turkey. In this study, 222 milk samples from 116 goats were collected from 10 flocks in the villages of Kavşit, Mutaflar, Tatarmemişler and Elderesi. Hair goat of 2-7 years of age that no history of vaccination against brucellosis. In this study, oligonucleotide primers specific for IS711, B. melitensis and B. abortus were used. After DNA extraction from the milk samples, PCR was used to detect B. melitensis and 50 positive results (22.5%) of milk samples of Hair Goat produced a 731 bp PCR band specific for B. melitensis.

Keywords:

Aydin, Brucella melitensis, DNA, brucellosis, disease prevalence, milk, polymerase chain reaction, public health, hair goat,

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