RAMNOLİPİT BİYOSÜRFEKTANLARININ ÜRETİMİNDE ENDÜSTRİYEL ATIK MADDELERİN KULLANILMASI VE RAMNOLİPİTLERİN UYGULAMA ALANLARI

Mikroorganizmalar tarafından üretilen yüzey ve iç yüzey gerilimi azaltabilen amfilik bileşiklere biyosürfektanlar denilmektedir. Biyosürfektanlar; çevre teknolojileri, sağlık, kozmetik, tarım ve petrol endüstrisi gibi endüstriyel alanlarda kullanılmaktadır. Pseudomonas aeruginosa tarafından sentezlenen yapısında ramnoz şekeri ve β-hidroksi dekanoik yağ asitleri içeren glikolipit yapısındaki biyosürfektanlara ramnolipitler denilmektedir. Bu araştırma yazımızda ramnolipit biyosürfektanların mikrobiyal üreticileri, sentezlenen ramnolipitlerin fiziko-kimyasal özellikleri ve endüstrideki uygulama alanlarını sunmaktır. Ayrıca ekonomik açıdan hemen hemen hiç değerlendirilmeyen, yenilenebilir ve ucuz endüstriyel atık maddelerin ramnolipit üretiminde karbon kaynağı olarak kullanılması üzerinde durulacaktır.

Anahtar Kelimeler:

Atık madde, Biyosürfektan, Ramnolipit, Pseudomonas aeruginosa

THE UTILIZE OF INDUSTRIAL WASTE SUBTRATE FOR RHAMNOLIPID BIOSURFACTANTS PRODUCTION AND APPLICATION POTENTIAL

Biosurfactants are amphiphilic compounds produced by microorganisms that are capable of decreasing surface and interfacial tensions. Biosurfactants are useful in environmental technology, medicine, cosmetic, agriculture, oil industry remediation of insoluble organic pollutions in soil and marine environmental. Pseudomonas aeruginosa produces and secretes rhamnose and β-hidroksi decanoic acid containing biosurfactants called rhamnolipids. In this review, we report are microbial producers, physico-chemical structure, microbial production, applications moreover they can be produced from various substrates, mainly renewable resources such as industry waste which are economical but have not been report in detail.

Keywords:

Industrial waste, Biosurfactants, Rhamnolipid, Pseudomonas aeruginosa,

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[1] Banat I.M., Makkar R.S., Cameotra S.S., (2000), Potential Commercial Applications of Microbial Surfactans, Applied Microbiology and Biotechnology, 53: 495-508.
[2] Van Dyke M.I., Lee H., Trevors J.T., (1991), Applications of Microbial Surfactants. Biotechnology, Advances, 9: 241-252.
[3] Maier RM., Soberon-Chavez G., (2000), Pseudomonas aeruginosa Rhamnolipids: Biosynthesis and Potential Application, Applied Microbiology and Biotechnology, 54: 625-633
[4] Sidal U., Kolonyaka N., Kurtonur C., (2000), Pseudomonas sp. ile Zeytinyağı Atığından Biyosürfektan Elde Edilmesi, Turkish Journal of Biology, 24: 611-625.
[5] Miller RM., (1995), Biosurfactant Facilitated Remediation of Metal Contaminated Soils, Environmental Health Perspectives, 103: 59-62.
[6] Mulligan N.C., (2005), Environmental Applications for Biosurfactants, Environmental Pollution 133 : 183-198.
[7] Lang S., (2002), Biological Amphiphiles (Microbial Biosurfactants), Current Opinion in Colloid Interface Science, 7: 12-20.
[8] Lang S., Wullbrandt D., (1999), Rhamnose lipids-Biosynthesis Microbial Production a Application Potential, Applied Microbiology and Biotechnology, 51 (1) : 22-32.
[9] Syldatk C., Wagner F., (1987) Production of Biosurfactants. Biosurfactants and Biotechnology. (Surfactants Science series Vol 25) Dekker, New York
[10] Mata-Sandoval JC., Karns J., Torrents A., (1999) High-performance Liquid Chromatography Method for The Characterization of Rhamnolipid Mixtures Produced by Pseudomonas aeruginosa UG2 on Corn oil, Journal of Chromatography A, 864: 211-220
[11] Giraund MF., Naismith JH., (2000), The Rhamnose Pathway, Current Opinion in Structural Biology, 10: 687-696
[12] Makar RS, Cameotra SS., (2002), An Uptake on use of Unconventional Substrates For Biosurfactant Production end Their New Applications, Applied Microbiology and Biotechnology 58:4258-434.
[13] Cameotra SS., Makkar RS., (1998), Synthesis of Biosurfactants in Extreme Conditions, Applied Microbiology and Biotechnology, 50: 520-529.
[14] Kosaric N., Cairns WL., Gray NCC., Stechey D., Wood J., (1984), The Role of Nitrogen in Multiorganism Strategies for Biosurfactant Production, Journal of The American Oil Chemistry Society, 61:1735-1743.
[15]Telefoncu A., (1995), Biyoteknoloji, Ege Üniversitesi Yayınları, Bornova- İzmir
[16] Haba E., Espuny MJ., Busquets M., Manresa M., (2000), Screening and Production of Rhamnolipids Pseudomonas aeruginosa 47 T2 NCIB 400044 from Waste Frying oils, Journal of Applied Microbiology, 88:379-387.
[17] Vollbrecht E., Heckmann R., Wray V., Nimtz M., Lang S., (1998), Production and Structure Elucidation of Di-oligasaccaride lipids (Biosurfactants) from Tsukamurella sp., Applied Microbiology and Biotechnology, 50: 530-537.
[18] Mecrada M.E., Manresa A., Robert M., Espuny MJ., De Andres C., Guinea J., (1993), Olive Oil Mill Effluent (OOME). New Substrate for Biosurfactants Production, Bioresource Technology, 43: 1-6.
[19] Mecrada M.E., Monleon L., De Andres C., Rodon I., Martinez E., Espuny MJ., Manresa A., (1996), Screening and Selection of Surfactant Producing Bacteria from Waste Lubricating Oil, Journal of Bacteriology, 81:161-168.
[20] Abolos A., Pinazo A., Infante MR., Casals M., Garcia F., Manresa A., (2001), Physicochemical and Antimicrobial Properties of New Rhamnolipids Produced by Pseudomonas aeruginosa AT10 from Soybean Oil Refinery Waste, Langmuir, 17:1367-1371.
[21] Fox SL., Bala GA., (2000) Production of Surfactant from Bacillus subtilis ATCC 21332 using Potato Substrates, Bioresource Technology, 75: 235-240.
[22] Patel R.M., Desai A.J., (1997), Biosurfactant Production by Pseudomonas aeruginosa GS3 from Molasses, Letter in Applied Microbiology, 25: 91-94.
[23] Sudhakar-Babu P., Vaidya AN., Bal AS., Kapur R., Juwarkar A., Khanna P., (1996),Kinetics of Biosurfactant Production by Pseudomonas aeruginosa strain BS2 from Industrial Waste, Biotechnology Letters, 18:263-268.
[24] Dubey K., Juvarkar A., (2001), Distillery and Curd Whey Waste as Visable Alternative Source for Biosurfactant Production, World of Journal of Microbiology and Biotechnology, 17:61-69
[25] Zhang Y., Miller RM., (1992), Enhanced Octodecane Dispersion and Biodegration by a Pseudomonas Rhamnolipid surfactant (Biosurfactant), Applied Environmental Microbiology, 58 (10): 3276-3282.
[26] Zhang Y., Miller RM., (1994), Effect of a Pseudomonas Rhamnolipid Biosurfactant on Cell Hydrophobicity and Biodegradation Octodecane, Applied Environmental Microbiology, 60: 2101-2106.
[27] Rahman KS., Banat IM., Thahira J., Thayumanavant., Lakshmanaperumalsamy P., (2002), Bioremediation of Gasoline Contaminated Soil by a Bacterial Consortium Amended with Poultry Litter, Coir Pith and Rhamnolipid Biosurfactant, Bioresources Technology, 81 (1):25-32.
[28] Chakrabarty A.M., (1985), Genetically Manipulated Microorganisms and Their Products in the Oil Service Industries, Trends Biotechnology, 3:32-38.
[29] Maier RM., Neilson JW., Artiola JF., Jordan FL., Glen EP., Descher SM., (2001), Remediation of Metal-Contaminated Soil and Sludge Using Biosurfactant Technology, International Journal of Occupation Medicinal Environment Health, 14 (3): 241-248.
[30] Bai GY., Brausseau ML., Miller RM., (1997), Biosurfactant Enhanced Removal of Residual Hydrocarbon from Soil, Journal of Contaminant Hydrology, 25: 157-170.
[31] Mulligans CN., Yong RN., Gibbs BF., (1999), On The Use of The Biosurfactants for The Removal of Heavy Metals from Oil Contaminated Soil, Environmental Progress, 18: 50-54.
[32] Herman DC., Artiola JF., Miller RM., (1995), Removal of Cadmium, Lead and Zinc from Soil by a Rhamnolipid Biosurfactant, Environmental Science and Technology, 29: 2202-2206.
[33] Tan H., Champion JT., Artiola JF., Brausseau ML., Miller RM., (1994), Complexation of Cadmium by a Rhamnolipid Biosurfactant, Environmental Science and Technology, 28: 2402-2406.
[34] Torrens JL., Herman DC., Miller-Maier RM., (1998), Biosurfactant (Rhamnolipid) Sorption and The Impact on Rhamnolipid-Facilitated Removal of Cadmium from Various Soil, Environmental Science and Technology, 32-776-781.
[35] Ochoa Loza FJ., Artiola JF., Maier RM., (2001), Stability Constants for The Complexation of Various Metals with a Rhamnolipid Biosurfactant, Journal of Environmental Quality, 30 (2): 479-485.
[36] Mulligan CN., Yong RN., Gibbs BF., (2001), Heavy Metal Removal From Sediments by Biosurfactants, Journals of Hazard Material, 30; 85 (1-2): 111-125.
[37] Stangellini ME., Miller RM., (1997), Biosurfactants: Their Identity and Potential Efficacy in Biological Control of Zoosporic Plant Pathogens, Plant Disease, 81: 4-12.
[38] Haferburg D., Hommel R., Kleber HP., Klug S., Schuster G., Zchienger HJ., (1987), Antiviral Activity of Rhamnolipids from Pseudomonas aeruginosa. Acta Biotechnology, 7: 353–356.
[39] Haba E., Pinazo A., Jauregui O., Espuny MJ., Infante MR., Manresa A., (2000), Physicochemical Characterization and Antimicrobial Properties of Rhamnolipids Produced by Pseudomonas aeruginosa 47 T2 NCBIM 40044, Biotechnology and Bioengineering, 81 (3): 316-322.