Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

The aim of the present study was to investigate total phenolic content and biological effects of methanolextracts from Cornus sanguinea L. leaves (LME) and fruits (FME). Total phenolic contents, COX-1/COX-2, α-glucosidase, AChE, tyrosinase inhibitory and DPPH radical scavenging effects of both extracts were investigated byusing spectrophotometric methods. The total phenolic contents of LME and FME were determined as 191.14 ± 4.84and 31.51 ± 2.68 mg GAE/g dry weight, respectively. LME inhibited COX-1 enzyme 70.71 ± 1.88% and 79.38 ± 0.92%at 50 and 100 μg/mL. LME had higher COX-1 and COX-2 inhibitory effects than that of FME. LME inhibited α-glucosidase stronger than positive control, acarbose. On the other hand, both extracts showed lower AChE inhibitionactions compared to positive control, galantamine. Moreover, LME had higher tyrosinase inhibitory effect than FME.Both extracts scavenged DPPH radical in a concentration-dependent manner. Also, LME had stronger scavengingeffect than that of FME. To our knowledge, current work is the first report on tyrosinase, AChE, as well as COX-1inhibitory properties of C. sanguinea. These results suggested that LME of C. sanguinea have a promising potential forthe treatment of several disorders but further studies are needed to support the this assumption.

___

  • [1] David L, Moldovan B, Baldea I, Olteanu D, Bolfa P, Clichici S, Filip GA. Modulatory effects of Cornus sanguinea L. mediated green synthesized silver nanoparticles on oxidative stress, COX-2/NOS2 and NFkB/pNFkB expressions in experimental inflammation in Wistar rats. Mater Sci Eng C. 2020; 110: 110709. [CrossRef]
  • [2] Stankovic MS, Topuzovic MD. In vitro antioxidant activity of extracts from leaves and fruits of common dogwood (Cornus sanguinea L.). Acta Bot Gallica. 2012; 159(1): 79-83. [CrossRef]
  • [3] Chamberlain DF. Cornus L. In: Davis PH (Ed.) Flora of Trukey and the East Aegean Islands, Vol IV. Edinburgh University Press, Edinburgh, 1972, pp.539-541.
  • [4] Popovic Z, Smiljanić M, Kostić M, Nikić P, Janković S. Wild flora and its usage in traditional phytotherapy (Deliblato Sands, Serbia, South East Europe). Indian J Tradit Knowl. 2014; 13: 9–35.
  • [5] Bulut G. Folk medicinal plants of Silivri (Istanbul, Turkey). Marmara Pharm J. 2011; 15: 25-29. [CrossRef]
  • [6] Saraç DU, Özkan ZC, Akbulut S. Ethnobotanic features of Rize/Turkey province. Biol Divers Conservat. 2013; 6: 57–66.
  • [7] Yousfbeyk F, Esmaiili T, Pashna Z, Hozori Z, Ghohari AR, Ostad SN, Amin GR. Antioxidant activity, total phenol and total anthocyanin contents of Cornus sanguinea L subsp australis. (C.A. Mey.) Jáv. J Med Plant. 2014; 13(49): 69- 74.
  • [8] Popović Z, Bajić-Ljubičić J, Matić R, Bojović S. First evidence and quantification of quercetin derivatives in dogberries (Cornus sanguinea L.). Turk J Biochem. 2017; 42(4): 513–518. [CrossRef]
  • [9] Oskay M, Sarı D. Antimicrobial screening of some Turkish medicinal plants. Pharm Biol. 2007; 45(3): 176-181. [CrossRef]
  • [10] Zhang L, Lv J. Sesquiterpenoids from Artemisia argyi and their COXs inhibitory activities. Fitoterapia 2019; 139: 104372. [CrossRef]
  • [11] Larsen BHV, Soelberg J, Jager AK. COX-1 inhibitory effect of medicinal plants of Ghana. S Afr J Bot. 2015; 99: 129– 131. [CrossRef]
  • [12] Tamgewagh UU, Kandhare AD, Honmore VS, Kadam PP, Khedkar VM, Bodhankar SL, Rojatkar SR. Antiinflammatory and antioxidant potential of guaianolide isolated from Cyathocline purpurea: Role of COX-2 inhibition. Int Immunopharmacol. 2017; 52: 110–118. [CrossRef]
  • [13] Ni M, Hu X, Gong D, Zhang G. Inhibitory mechanism of vitexin on α-glucosidase and its synergy with acarbose. Food Hydrocoll. 2020; 105: 105824. [CrossRef]
  • [14] Zhao L, Wen L, Lu Q, Liu R. Interaction mechanism between α-glucosidase and a-type trimer procyanidin revealed by integrated spectroscopic analysis techniques. Int J Biol Macromol. 2020; 143: 173–180. [CrossRef]
  • [15] Ragab HM, Teleb M, Haidar HR, Gouda N. Chlorinated tacrine analogs: Design, synthesis and biological evaluation of their anti-cholinesterase activity as potential treatment for Alzheimer’s disease. Bioorg Chem. 2019; 86: 557–568. [CrossRef]
  • [16] Imran M, Irfan A, Ibrahim M, Assiri MA, Khalid N, Ullah S, Al-Sehem AG. Carbonic anhydrase and cholinesterase inhibitory activities of isolated flavonoids from Oxalis corniculata L. and their first-principles investigations. Ind Crop Prod. 2020; 148: 112285. [CrossRef]
  • [17] Bilska A, Kobus-Cisowska J, Kmiecik D, Danyluk B, Kowalski R, Szymanowska D, Gramza-Michlowska A, Szczepaniak O. Cholinesterase inhibitory activity, antioxidative potential and microbial stability of innovative liver pâté fortified with rosemary extract (Rosmarinus officinalis). Electron J Biotechn. 2019; 40: 22–29. [CrossRef]
  • [18] Sari S, Barut B, Özel A, Şöhretoğlu D. Tyrosinase inhibitory effects of Vinca major and its secondary metabolites: Enzyme kinetics and in silico inhibition model of the metabolites validated by pharmacophore modeling. Bioorg Chem. 2019; 92: 103259. [CrossRef]
  • [19] Şöhretoğlu D, Sari S, Barut B, Özel A. Tyrosinase inhibition by some flavonoids: Inhibitory activity, mechanism by in vitro and in silico studies. Bioorg Chem. 2018; 81: 168–174. [CrossRef]
  • [20] Popovic Z, Matic R, Bajic-Ljubicic J, Tesevic V, Bojovic S. Geographic variability of selected phenolic compounds in fresh berries of two Cornus species. Trees 2018; 32: 203-214. [CrossRef]
  • [21] Kim M, Choi S, Lee P, Hur J. Neochlorogenic acid inhibits lipopolysaccharide-induced activation and proinflammatory responses in BV2 microglial cells. Neurochem Res. 2015; 40: 1792–1798. [CrossRef]
  • [22] Mandour Y, Handoussa H, Swilam N, Hanafi R, Mahran L. Structural docking studies of COX-II inhibitory activity for metabolites derived from Corchorus olitorius and Vitis vinifera. Int J Food Prop. 2016; 19: 2377–2384. [CrossRef]
  • [23] Comolada M, Camuesco D, Sierra S, Ballester I, Xaus J, Galvez, Zarzuelo A. In vivo quercitrin anti‐ inflammatory effect involves release of quercetin, which inhibits inflammation through down‐ regulation of the NF‐ κB pathway. Euro J Immun. 2005; 35: 584–592. [CrossRef]
  • [24] Ning Z, Zhai L, Huang T, Peng J, Hu D, Xiao H, Wen B, Lin C, Zhao L, Bian Z, Identification of α-glucosidase inhibitors from cyclocarya paliurus tea leaves using UF-UPLC-Q/TOF-MS/MS and molecular docking. Food Funct. 2019; 10: 1893-1902. [CrossRef]
  • [25] Truba J, Stanislawska I, Walasek M, Wieczorkowska W, Wolinski K, Buchholz T, Melzig MF, Czerwinska ME. Inhibition of digestive enzymes and antioxidant activity of extracts from fruits of Cornus alba, Cornus sanguinea subsp. hungarica and Cornus florida–A comparative study. Plants 2020; 9: 122. [CrossRef]
  • [26] Bhakta HK, Park CH, Yokozawa T, Tanaka T, Jung HA, Choi JS. Potential anti-cholinesterase and β-site amyloid precursor protein cleaving enzyme 1 inhibitory activities of cornuside and gallotannins from Cornus officinalis fruits. Arch Pharm Res. 2017; 40: 836–853. [CrossRef]
  • [27] An YA, Hwang JY, Lee JS, Kim YC. Cornus officinalis methanol extract upregulates melanogenesis in melan-a cells. Toxicol Res. 2015; 31(2): 165-172. [CrossRef]
  • [28] Kandia S, Linton Charles A. Statistical comparative study between the conventional DPPH% spectrophotometric and dropping DPPH% analytical method without spectrophotometer: Evaluation for the advancement of antioxidant activity analysis. Food Chem. 2019; 287: 338-345. [CrossRef]
  • [29] Kahkönen MP, Hopia AI, Vuorela HJ, Rauha J, Pihlaja K, Kujala TS, Heinonen M. Antioxidant activity of plant extracts containing phenolic compounds. J Agric Food Chem. 1999; 47(10): 3954-3962. [CrossRef]
  • [30] de Oliveira ADT, de Miranda MDS, Jacob ITT, da Cruz Amorim CA, de Moura RO, da Silva SAS, Soares MBP, de Almeida SMV, de Lima Souza TRC, de Oliveira JF, da Silva TG, de Melo, CRL, Moreira DRM, de Lima MC. Synthesis, in vitro and in vivo biological evaluation, COX-1/2 inhibition and molecular docking study of indole-Nacylhydrazone derivatives. Bioorg Med Chem. 2018; 26: 5388-5396. [CrossRef]
  • [31] Barut B, Barut EN, Engin S, Özel A, Sezen FS, Investigation of the antioxidant, α-glucosidase inhibitory, antiinflammatory, and DNA protective properties of Vaccinium arctostaphylos L. Turk J Pharm Sci. 2019; 16: 175-183. [CrossRef]
  • [32] Barut EN, Barut B, Engin S, Yıldırım S, Yaşar A, Turkis S, Özel A, Sezen FS. Antioxidant capacity, antiacetylcholinesterase activity and inhibitory effect on lipid peroxidation in mice brain homogenate of Achillea millefolium. Turk J Biochem. 2017; 42: 493-502. [CrossRef]
  • [33] Sellitepe HE, Doğan İS, Eroğlu G, Barut B, Özel A. Synthesis, characterization and investigation of cholinesterase enzyme inhibition and antioxidant activities of some 4-aryl-1,4-dihydropyridine derivatives. J Res Pharm. 2019; 23(4): 608-616. [CrossRef]
Journal of research in pharmacy (online)-Cover
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: Marmara Üniversitesi
Sayıdaki Diğer Makaleler

Laxative effect of the crude methanolic extract of Polyalthia suberosa (Roxb.) Thwaites in mice

Mst. Irin AKTER, Md. Abdullah AZIZ, Nelufar YASMEN

Host-guest inclusion complex of desloratadine with 2-(hydroxy)propyl-β-cyclodextrin (HP-β-CD): Preparation, binding behaviors and dissolution properties

Gülsel YURTDAŞ KIRIMLIOĞLU

Development of lornoxicam multiparticulate sustained release drug delivery system using copal gum-pectin and optimization by applying central composite design

Mulchand SHENDE, Priya DESHMUKH

Morinda citrifolia Linn. (Noni) fruit extract attenuates ethanol seeking behavior in mouse runway paradigm

Yasmin KHAN, Vijayapandi PANDY

Characterization of antioxidant properties of strawberry tree (Arbutus unedo L.) and trace elements determination

Gülbin ERDOĞAN, Tuğba UYSAL

Development and characterization of self-nanoemulsifying drug delivery system (SNEDDS) formulation for enhancing dissolution of fenofibric acid

Wira Noviana SUHERY, Yeyet Cahyati SUMIRTAPURA, Jessie Sofia PAMUDJI, Diky MUDHAKIR

Design, synthesis and biological evaluation of new bis(thiosemicarbazone) derivatives as potential targeted anticancer agents for non-small cell lung cancer

Mehlika Dilek ALTINTOP, Ahmet ÖZDEMİR, Belgin SEVER, Gülşen AKALIN ÇİFTÇİ

Assessment of patient relevant outcomes in knee osteoarthritis patients using the Knee Injury and Osteoarthritis Outcome Score (KOOS) scale

VinodKumar MUGADA, Pujitha KONDRU, Sai Navya MANDA, Mahalakshmi VEGI, Smruthi KAKADA

Protective effects of quercetin against cisplatin induced urogenital organ toxicity

Özge ÇEVİK, Göksel ŞENER, Feriha ERCAN, Tarık Emre ŞENER, Selin ÇADIRCI, M. Kutay KÖROĞLU, Selin ŞAKARCAN

Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

Didem ŞÖHRETOĞLU, Burak BARUT