Mst. Irin AKTER, Md. Abdullah AZIZ, Nelufar YASMEN

Laxative effect of the crude methanolic extract of Polyalthia suberosa (Roxb.) Thwaites in mice

Polyalthia suberosa (Roxb.) Thwaites is traditionally used as abortifacient, laxative, febrifuge, analgesic,anti-HIV as well as filler’s of tooth cavities. The present study was directed to evaluate laxative activity of themethanolic extract of Polyalthia suberosa (Roxb.) Thwaites leaves (PSM). A variety of tests including laxative activitytest, laxative activity on loperamide-induced constipation in mice and gastrointestinal motility test in mice wereutilised to evaluate the laxative activity of PSM. PSM at 200 and 400 mg/kg doses, expressed laxative activity in Swissalbino mice. Laxative effect of PSM evaluated by laxative activity test exposes that PSM possesses laxative activity atboth doses. PSM also showed effectiveness against loperamide induced constipation. In addition, this extractproduced significant intestinal transit (*P˂0.05 vs. control) at both doses. The results of this study provide support forthe traditional use of Polyalthia suberosa (Roxb.) Thwaites leaves as a laxative.

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[1] Nafiu MO, Abdulsalam TA, Jimoh RO, Kazeem MI. Ameliorative effect of Lecaniodiscus cupanioides (Sapindaceae) aqueous root extract in loperamide induced constipated rats. Trop J Pharm Res. 2015; 14: 1057-1062. [CrossRef]
[2] Higgins PD, Johanson JF. Epidemiology of constipation in North America: a systematic review. Am J Gastroenterol. 2004; 99: 750-759.
[3] Ashafa AOT, Sunmonu TO, Abass AA, Ogbe AA. Laxative potential of the ethanolic leaf extract of Aloe vera (L.) Burm. f. in Wistar rats with loperamide-induced constipation. J Nat Pharm. 2011; 2: 158-162.
[4] Golla U, Gajam PK, Bhimathati SS. Evaluation of diuretic and laxative activity of hydro- alcoholic extract of Desmostachya bipinnata (L.) Stapf in rats. J Integr Med. 2014; 12: 372-378. [CrossRef]
[5] Méité S, Bahi C, Yéo D, Datté JY, Djaman JA, N’guessan DJ. Laxative activities of Mareya micrantha (Benth.) Müll. Arg. (Euphorbiaceae) leaf aqueous extract in rats. BMC Complement Altern Med. 2010; 10: 7. [CrossRef]
[6] Johanson JF, Kralstein J. Chronic constipation: a survey of the patient perspective. Aliment Pharmacol Ther. 2007; 25: 599-608. [CrossRef]
[7] Bengtsson M, Ohlsson B. Psychological well-being and symptoms in women with chronic constipation treated with sodium picosulphate. Gastroenterol Nurs. 2005; 28: 3-12. [CrossRef]
[8] Kew Science. Plants of the world online. Accessed date: June 21, 2020.
[9] Mazumdar R, Rahman MH, Rahman MM, Chowdhury MAR, Hossain MA, Ahmed MS, Gupta PD. Phytochemical and pharmacological investigation of Polyalthia suberosa Roxb. Int J Innov Pharm Sci Res. 2016; 4: 693-708.
[10] Stuart GU. Duhat-matsing, Philippine medicinal plants. Accessed date: June 21, 2020.
[11] Kumar ESS, Krishnan SG, Murugesan K, Shailajakumari S. Notes on Polyalthia malabarica (Bedd.) I.M. Turner and Polyalthia suberosa (Roxb.) Thwaites (Annonaceae). Int J Bot Stud. 2016; 1(4): 27-28.
[12] Khare CP, Indian medicinal plants: an illustrated dictionary, 1st ed., Springer-Verlag, New York, USA 2007.
[13] Bellah SF, Ahmed F, Rahman AA, Shahid IZ, Hossen SMM. Preliminary phytochemical, anti-bacterial, analgesic, anti-diarrhoeal and cytotoxic activity of methanolic extract of Polyalthia suberosa leaves. Int J Pharm Sci Res. 2012; 5: 1322-1326. [CrossRef]
[14] Labu ZK, Makshud M, Basir S, Uddin J, Biswas S, Sultana N. Study of phytochemical screening, antioxidant, antidiarrhoeal and analgesic activities of hydromethanol extracts of Polyalthia suberosa (Roxb.). J Biomed Pharma Res. 2013; 2(3): 52-63.
[15] Li HY, Sun NJ, Kashiwada Y, Sun L, Snider JV, Cosentino LM, Lee KH. Anti-AIDS agents, 9. Suberosol, a new C31 lanostane-type triterpene and anti-HIV principle from Polyalthia suberosa. J Nat Prod. 1993, 56(7): 1130-1133. [CrossRef]
[16] Yasmen N, Aziz MA, Tajmim A, Akter MI, Hazra AK, Rahman SMM. Analgesic and anti-inflammatory activities of diethyl ether andn-hexane extract of Polyalthia suberosa leaves. Evid Based Complement Alternat Med. 2018; 5617234. [CrossRef]
[17] Saito T, Mizutani F, Iwanaga Y, Morikawa K, Kato H. Laxative and antidiarrheal activity of polycarbophil in mice and rats. Jpn J Pharmacol. 2002, 89(2): 133-141. [CrossRef]
[18] Gaginella TS, Bass P. Laxatives: An update on mechanism of action. Life Sci. 1978; 23: 1001-1009. [CrossRef]
[19] Sharma S, Paliwal S, Dwivedi J, Tilak A. First report on laxative activity of Citrullus lanatus. Pharmacologyonline. 2011; 2: 790-797.
[20] Huizinga JD, Ambrous K, Der-Silaphet T. Co-operation between neural and myogenic mechanisms in the control of distension-induced peristalsis in the mouse small intestine. J Physiol. 1998; 506: 843-856. [CrossRef]
[21] Waterman SA, Costa M. The role of enteric inhibitory motoneurons in peristalsis in the isolated guinea-pig small intestine. J Physiol. 1994; 477: 459-468.
[22] Otshudi AL, Vercruysse A, Foriers A. Contribution to the ethnobotanical, phytochemical and pharmacological studies of traditionally used medicinal plants in the treatment of dysentery and diarrhoea in Lomela area, Democratic Republic of Congo (DRC). J Ethnopharmacol. 2000; 71: 411-423. [CrossRef]
[23] Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council. Guide for the Care and Use of Laboratory Animals. National Academy Press, Washington, 1996, pp. 65, 21–48.
[24] Mascolo N, Izzo AA, Avtore G, Barboto F, Capasso F. Nitric oxide and castor oil induced diarrhea. J Pharmacol Exp Therap. 1994; 268: 291-295.