Morinda citrifolia Linn. (Noni) fruit extract attenuates ethanol seeking behavior in mouse runway paradigm

In this study, we used methanolic extract of Morinda citrifolia Linn. fruit (MMC) in order to find its effectagainst ethanol seeking behavior in a modified mouse runway paradigm. During conditioning/acquisition animalswere treated with accelerated doses of ethanol (0.5-4 g/kg, i.p.) for 5 days (Day 1- Day 5). Then, ethanol seekingbehavior was assessed on Day 6 (postconditioning test). Ethanol seeking behavior was confirmed in ethanol controlgroup in which the run time to reach the target box was significantly decreased. The test drug MMC (1, 3, and 5 g/kg,p.o.) caused a significant reversal of the ethanol seeking behavior by increasing the run time to reach the goal box asseen with the reference drug acamprosate (ACAM; 300 mg/kg, p.o.) in mice. After postconditioning test, nonrewardedabolishment assays were performed for a period of five days (Day 7 –Day 11). After 5 days of abolishment(abstinence), a priming dose of ethanol (the highest dose at 1/5th portion applied in control (i.e. 0.8 g/kg, i.p.)) inhouse cage significantly reinstated the ethanol-seeking behavior. Interestingly, MMC at a higher dose (5 g/kg, p.o.)showed significant halt on ethanol-induced reinstatement in mice as shown with the reference drug ACAM (300mg/kg, p.o.). The outcome of this study reveals that MMC alleviated ethanol seeking behaviour in modified mouserunway paradigm and it could be effectively used to treat alcohol dependence.

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