Characterization of antioxidant properties of strawberry tree (Arbutus unedo L.) and trace elements determination

Characterization of antioxidant properties of strawberry tree (Arbutus unedo L.) and trace elements determination

Strawberry tree (Arbutus unedo L.) is a species that generally grows in the Mediterranean region and coasts and has been consumed as food since ancient times. Its fruits are mostly used in alcoholic beverages and various jams and desserts. Its fruits and leaves contain different phenolic compounds, because of this it is used in traditional folk medicine. They have different biological properties like, anti-diabetic, anti-inflammatory, urinary antiseptic, anti-hypertension, astringent, anti-diarrheal due to their high quantity of tannins. In this study, we aimed that to determine the total antioxidant capacity of fruits and leaves and trace element contents of Arbutus unedo L. which were collected in Yalova (TURKEY). The antioxidant activities of samples were examined by ferric reducing/antioxidant power (FRAP) method, cupric reducing antioxidant capacity (CUPRAC) method and trolox equivalent antioxidant capacity (TEAC) assay with ABTS radical cation. In the samples of the amount of total phenolic and tannins contents were determined using the Folin-Ciocalteu reagent (FCR) method. In adddition to, we determined the concentrations of Al, Cd, Cu, Cr, Fe, Mn, Pb, Zn in the samples by using ICP-MS. As a result, Arbutus unedo L. is a good source of antioxidants and minerals, it was stated that single antioxidant activity test would present limited information about the power of the antioxidant capacity and it was emphasized that use of combination of various methods should be used in assessing the antioxidant activities in vitro. It has been determined that the trace elements are within the determined limits.

___

  • [1] Aeschbach R, Halliwell B, Löliger J, Aruoma OI. The characterization of antioxidants. Food Chem Toxicol. 1995; 33: 601-617. [CrossRef]
  • [2] Cadenas E, Packer L. Handbook of Antioxidants. Marcel Dekker, New York-Basel. 2002; 0-8247-0547-5.
  • [3] Fang YZ, Yang S, Wu G. Free radicals, antioxidants, and nutrition. Biochem Mol Biol. 2002; 18 (10): 872–879. [CrossRef]
  • [4] Kim YJ, Lee KW, Kim D, Lee HJ, Chun Y. Major phenolics in the apple their contribution to the total antioxidant capacity. J Agric Food Chem. 2003; 51(22): 6516-6520. [CrossRef]
  • [5] P. G. Pietta. Flavonoids as antioxidants. J Nat Prod. 2000; 63(7): 1035–1042. [CrossRef]
  • [6] Halliwell B, Aruoma OI. DNA damage by oxygen-derived species: Its mechanisms and measurement in mammalian systems. FEBS Letters. 1991; 281: 9-19. [CrossRef]
  • [7] Özcan MM, Hacıseferoğulları H. The Strawberry (Arbutus unedo L.) fruits: Chemical composition, physical properties and mineral contents. J Food Eng. 2007; 78(3): 1022–1028. [CrossRef]
  • [8] Laouicha S, Senator A, Kherbache K, Bouriche H. Total phenolic contents and antioxidant properties of algerian Arbutus unedo L. extracts. J Drug Deliv. 2020; 10(3-s):159-168. [CrossRef]
  • [9] Orak HH, Aktas T, Yagar H, İsbilir SS, Ekinci N, Sahin FH. Effects of hot air and freeze drying methods on antioxidant activity, colour and some nutritional characteristics of strawberry tree (Arbutus unedo L) fruit. Food Sci Technol Int. 2012; 18(4): 391-402. [CrossRef]
  • [10] Bouyahya A, Moussaoui NE, Abrini J, Bakri Y, Dakka N. Determination of Phenolic Contents, Antioxidant and Antibacterial Activities of Strawberry Tree (Arbutus unedo L.) Leaf Extracts. Br Biotechnol J. 2016; 14(3): 1-10. [CrossRef]
  • [11] Miguel MG, Faleiro ML, Guerreiro AC, Antunes MD. Arbutus unedo L.: Chemical and Biological Properties. Molecules. 2014; 19: 15799-15823. [CrossRef]
  • [12] Pabuçcuoğlu A, Kıvçak B, Baş M, Mert T. Antioxidant activity of Arbutus unedo L. leaves. Fitoterapia. 2003; 74(6): 597-599. [CrossRef]
  • [13] Fortalezas S, Tavares L, Pimpao R, Tyagi M, Pontes V, Alves PM, McDougall G, Stewart D, Ferreira RB, Santos CN. Antioxidant properties and neuroprotective capacity of strawberry tree fruit (Arbutus unedo). Nutrients. 2010; 2: 214-229. [CrossRef]
  • [14] Karıkas GA, Euerby MR, Waigh RD. Constituents of the stems of Arbutus unedo L. Planta Medica. 1986; 53(2): 223-224.
  • [15] Guimaraes R, Barros L, Duenas M, Carvalho AM, Queiroz MJRP, Celestino Santos-Buelga C, Ferreira ICFR. Characterisation of phenolic compounds in wild fruits from Northeastern Portugal. Food Chem. 2013; 141: 3721-3730. [CrossRef]
  • [16] Nenadis N, Llorens L, Koufogianni A, Diaz B, Font J, Gonzales JA, Verdaguer D. Interactive effects of UV radiation and reduced precipitation on the seasonal leaf phenolic content/composition and the antioxidant activity of naturally growing Arbutus unedo plants. J Photochem Photobiol B. 2015; 153: 435-444. [CrossRef]
  • [17] Küçükbay Z, Kuyumcu E. Determination of trace element contents of Thymus species from Turkey. Turk J Chem. 2010; 34: 911-919. [CrossRef]
  • [18] Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agr Food Chem. 2005; 53: 1841-1856. [CrossRef]
  • [19] Büyüktuncel E. Main spectrophotometric methods for the determination of total phenolic content and antioxidant capacity. Marmara Pharm J. 2013; 17: 93-103. [CrossRef]
  • [20] Apak R, Güçlü K, Özyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. J Agr Food Chem. 2004; 52: 7970-7981. [CrossRef]
  • [21] Miller NJ, Rice Evans CA, Davies MJ, Gopiathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clinical Sci. 1993; 84: 407-412. [CrossRef]
  • [22] Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": the FRAP assay. Anal Biochem. 1996; 239(1): 70-76. [CrossRef]
  • [23] Pellegrini N, Serafini M, Colombi B, Del Rio D, Salvatore S, Bianchi M, Brighenti F. Total antioxidant capacity of plant foods, beverages and oils consumed in Italy assessed by three different in vitro assays. J Nutr. 2003; 133(9): 2812-2819. [CrossRef]
  • [24] Singleton VL, Joseph A, Rossi, JRJA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic. 1965; 16: 144-158.
  • [25] Slinkard K, Singleton VL. Total phenols analysis: automation and comparison with manual methods. Am J Enology Vitic. 1977; 28: 49-55. [CrossRef]
  • [26] Alkaya DB, Seyhan SA, Öztürk BN. Influence of extraction method on antioxidant properties of Rheum ribes root extract. Ovidius University Annals of Chemistry. 2019; 30(1): 44-47. [CrossRef]
  • [27] Perez-Jimenez J, Saura-Calixto F. Effect of solvent and certain food constituents on different antioxidant capacity assays. Food Res Int. 2006; 39(7): 791–800. [CrossRef]
  • [28] Finotti E, Majo D. Influence of solvents on the antioxidant property of flavonoids. Mol Nutr Food Res. 2003; 47(3): 186–187. [CrossRef]
  • [29] Dorman HJD, Peltoketo A, Hiltunen R, Tikkanen MJ. Characterisation of the antioxidant properties of de-odourised aqueous extracts from selected Lamiaceae herbs. Food Chem. 2003; 83(2): 255–262. [CrossRef]
  • [30] Balkan İA, Taşkın T, Şah EA, Akaydın G, Yeşilada E. Comparative study of the anti-inflammatory, antioxidant and urease inhibitory activities of Eryngium kotschyi Boiss. and E. campestre L. var. virens (Link) Weins extracts. J Res Pharm. 2020; 24(3): 399-409. [CrossRef]
  • [31] Trouillas P, Calliste CA, Allais DP, Simon A, Marfak A, Delage C, Duroux JL. Antioxidant, anti-inflammatory and antiproliferative properties of sixteen water plant extracts used in the Limousin countryside as herbal teas. Food Chem. 2003; 80(3): 399–407. [CrossRef]
  • [32] Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem. 2004; 85: 231-237. [CrossRef]
  • [33] WHO, Quality control methods for medicinal plant materials, Geneva, Switzerland. 1998. [CrossRef]
  • [34] MacDonald-Wicks LK, Wood LG, Garg ML. Methodology for the determination of biological antioxidant capacity in vitro: A review. J Sci Food Agr. 2006; 86(13): 2046-2056. [CrossRef]
  • [35] Karasakal A. Evaluation of antioxidant activities of Brassica napus’s seeds by CUPRAC, ABTS/Persulphate and DMPD methods. Marmara Pharm J. 2015; 19: 153-158. [CrossRef]
  • [36] Taskın T, Levent M, Şahin T. Investigation of the effects of biological activity and steeping time of four different black teas commercially valuable in Turkey. Clin Exp Health Sci. 2019; 9: 283-287. [CrossRef]
  • [37] Şavşatlı Y. The effects of wheatgrass length on antioxidant activity and total phenolic content in wheatgrass Triticum spp. Turk J Agric For. 2020; 44(3): 271-277. [CrossRef]
  • [38] Başkan KS, Tütem E, Özer N, Apak R. Spectrophotometric and chromatographic assessment of contributions of carotenoids and chlorophylls to the total antioxidant capacities of plant foods. J Agric Food Chem. 2013; 61(47): 11371–11381. [CrossRef]
  • [39] Yılmaz DÇ, Seyhan SA. Antioxidant potential of Cydonia oblonga Miller leaves. Istanbul J Pharm. 2017; 47(1): 9-11. [CrossRef]
  • [40] Mendes L, Freitas V, Baptista P, Carvalho M. Comparative antihemolytic and radical scavenging activities of strawberry tree (Arbutus unedo L.) leaf and fruit. Food Chem Toxicol. 2011; 49: 2285–2291. [CrossRef]
  • [41] Oliveiraa I,Baptistaa P, Malheiroa R, Casalb S, Bentoa A, Pereir JS. Comparative antihemolytic and radical scavenging activities of strawberry tree (Arbutus unedo L.) leaf and fruit. Food Res Int. 2011; 44: 1401-1407 [CrossRef]
  • [42] Çakmak H, Bozdoğan N, Turkut MG, Kumcuoğlu S, Tavman Ş. Evaluation of drying kinetics of Arbutus unedo L. fruit and determination of quality characteristics. Gıda. 2016; 41(4): 227-234. [CrossRef]
  • [43] Moualek I, Aiche GI, Guechaoui NM, Lahcene S, Houali K. Antioxidant and anti-inflammatory activities of Arbutus unedo aqueous extract. Asian Pac J Trop Biomed. 2016; 6(11): 937-944. [CrossRef]
  • [44] Vidrih R, Hribar J, Prgomet Z, Ulrih NP. The physico-chemical properties of strawberry tree (Arbutus unedo L.) fruits. Croat J Food Sci Technol. 2013; 5(1): 29-33.
Journal of research in pharmacy (online)-Cover
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: Marmara Üniversitesi
Sayıdaki Diğer Makaleler

Morinda citrifolia Linn. (Noni) fruit extract attenuates ethanol seeking behavior in mouse runway paradigm

Yasmin KHAN, Vijayapandi PANDY

Vortioxetine improved social and cognitive deficits in acute ketamine model of schizophrenia in rats

Gökhan ÜNAL, Mehmet TAŞKIRAN

Assessment of patient relevant outcomes in knee osteoarthritis patients using the Knee Injury and Osteoarthritis Outcome Score (KOOS) scale

VinodKumar MUGADA, Pujitha KONDRU, Sai Navya MANDA, Mahalakshmi VEGI, Smruthi KAKADA

What we know about COVID-19 and its treatment

Göksel ŞENER, Zatiye Ayça ÇEVİKELLİ YAKUT, Sinem ŞAKARCAN

Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

Cem ÖNAL, Şerife Evrim KEPEKÇİ TEKKELİ

Design, synthesis and biological evaluation of new bis(thiosemicarbazone) derivatives as potential targeted anticancer agents for non-small cell lung cancer

Mehlika Dilek ALTINTOP, Ahmet ÖZDEMİR, Belgin SEVER, Gülşen AKALIN ÇİFTÇİ

Design, synthesis, antifungal activity, and QM/MM docking study of two azole derivatives with indole ring

Suat SARI, Didem KART

Total phenolic content, cyclooxygenases, α-glucosidase, acetylcholinesterase, tyrosinase inhibitory and DPPH radical scavenging effects of Cornus sanguinea leaves and fruits

Didem ŞÖHRETOĞLU, Burak BARUT

Host-guest inclusion complex of desloratadine with 2-(hydroxy)propyl-β-cyclodextrin (HP-β-CD): Preparation, binding behaviors and dissolution properties

Gülsel YURTDAŞ KIRIMLIOĞLU

Experimental design approach for development of cocrystals and immediate release cocrystal tablet of atorvastatin calcium for enhancement of solubility and dissolution

Hemangi R. TRIVEDI, Dhananajay S. BORKAR, Prashant K. PURANIK