Oksana BUCHKO, Viktoriia HAVRYLIAK, Andriy PYLYPETS, Taras BUCHKO

Effect of food supplement of humic origin on the hematological and biochemical parameters in the Cr (VI) exposed rats

The study aimed to investigate the effect of Humilid, a biologically active food supplement, onbiochemical and hematologic parameters in the blood of rats exposed to hexavalent Chromium. Wistar male rats wereused in experiments. All animals were divided into 4 groups. Animals of experimental groups D2 and D3 received a1% solution of food supplement Humilid in a dose of 2 ml/kg of body weight during 28 days. Rats of groups D1 andD2 were injected with potassium bichromate (K2Cr2O7) in the dose of 2 mg Cr (VI)/kg of body weight from 14 days ofthe experiment. Animals of the C group were administered a 0.9% NaCl solution for 14 days. Data were analyzedusing the ANOVA test. It has been found that administration of potassium bichromate caused the cytotoxic effect onrat’s organism, inhibition of the synthetic function of the liver, hypoglycemic effect, toxic anemia and leukocytosis.The treatment with humic substances provided a marked normalization of the biochemical and hematologicalparameters in the blood of chromium-exposed animals. Biologic active supplement Humilid reduces the toxic effect ofchromium on the rat body, normalizes metabolism, and exhibits hepatoprotective and adaptogenic effects. Ourfindings show that Humilid supplementation may be helpful in the abrogation of heavy metal toxicity.

PDF

___

[1] Mishra S, Bharagava RN. Toxic and Genotoxic effects of hexavalent chromium in environment and its bioremediation strategies. J Environ Sci Health C Env Carc Ecotoxicol Rev. 2016; 34(1): 1-32. [CrossRef]
[2] Mihaylova IV, Smolyagin AI, Krasikov SI, Karaulov AV. Complex assessment of chromium on rats: immunology, biochemistry, trace elements. Immunologiya. 2015; 36(5): 300–305. [article in Russian with an abstract in English]
[3] Ray RR. Adverse haematological effects of hexavalent chromium: an overview. Interdiscip Toxicol. 2016; 9(2): 55– 65. [CrossRef]
[4] Jeevana LM, Srikanth MK, Gopala RA, Anudeep RM. Haematological study in hexavalent chromium toxicity in female wistar rats and its progeny. The Pharma Innov J. 2018; 7(1): 35-38.
[5] Stepchenko LM, Shaidek L, Novik V, Sotnikova OP, Haluzina LI. Dosiahnennia ta perspektyvy zastosuvannia huminovykh rechovyn u silskomu hospodarstvi. Dnipro: Astra-Prynt; 2017.
[6] Nurten G, Umit P, Hakan B. Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital J Anim Sci. 2010; 9: 390-393. [CrossRef]
[7] Wang Q, Ying J, Zou P, Zhou Y, Wang B, Yu D, Li W, Zhan X. Effects of Dietary Supplementation of Humic Acid Sodium and Zinc Oxide on Growth Performance, Immune Status and Antioxidant Capacity of Weaned Piglets. Animals. 2020; 10(11): 2104. [CrossRef]
[8] Balakrishnan R, Satish Kumar CS, Rani MU, Srikanth MK, Boobalan G, Reddy AG. An evaluation of the protective role of α-tocopherol on free radical induced hepatotoxicity and nephrotoxicity due to chromium in rats. Indian J Pharmacol. 2013; 45: 490-495. [CrossRef]
[9] Lebedeva EI, Myadelets OD, Kichigina TN, Grushin VN. Influence of toxic liver cyrosis on the content of general protein and protein fractions in serum of rat blood. J New Med Tech, eEdition. 2018; 2: 144-150. [CrossRef]
[10] Reham AA, Mounes HAM, Ahmed KM. Use of humic acid and Yucca extract as a benefactor on water quality and their impact on some hematological and histological parameters of Oreochromis niloticus. Egypt J Aquatic Biol Fish. 2018; 22(5): 447-460.
[11] Verigo NS, Ryzhkovaskaya EL, Kuznetsova TE, Ulashchik VS. Izmenenie strukturno-funkczionalnogo sostoyaniya pecheni u krys s gepatitom pod vliyaniem malomineralizovannoj mineralnoj vody s povyshennym soderzhaniem guminovykh kislot. Russian Journal of Physiotherapy, Balneology and Rehabilitation. 2013; 12(5): 4-10.
[12] Shil K, Pal S. Metabolic adaptability in hexavalent chromium-treated renal tissue: an in vivo study. Clin Kidney J. 2018; 11(2): 222–229. [CrossRef]
[13] Hegazy R, Salama A, Mansour D, Hassan A. Renoprotective effect of lactoferrin against chromium-induced acute kidney injury in rats: Involvement of IL-18 and IGF-1 Inhibition. PLoS ONE. 2016; 11(3): 1-18. [CrossRef]
[14] Burmas NI. The state of antioxidant system and bile formation function in the organism of rats affected by hexavalent chromium compounds. Med Clin Chem. 2016; 18(1): 89-93. [article in Ukranian with an abstract in English] [CrossRef]
[15] Buchko O, Havryliak V. Effect of the Supplement of Humic Origin on the Free Radical Processes and Histological Changes in the Tissues of Rats Affected by Chromium (VI). Biointerface Res Appl Chem. 2021; 11(3): 10996 – 11008. [CrossRef]
[16] Sahin A, Iskender H, Terim Kapakin KA, Altinkaynak K, Hayirli A, Gonultas A, Kaynar O. The effect of humic acid substances on the thyroid function and structure in lead poisoning. Braz J Poultry Sci. 2016; 18(4): 649-654. [CrossRef]
[17] Szabó J, Vucskits AV, Berta E, Andrásofszky E, Bersényi A, Hullár I. Effect of fulvic and humic acids on iron and manganese homeostasis in rats. Acta Vet Hung. 2017; 65(1): 66–80. [CrossRef]
[18] Lavrik NL, Ilyitcheva ТN. Study of the Influence of Humic Acid Macromolecules on the Structure of Erythrocytes of Some Animals by the Method of Absorption. Bioph Chem. 2019; 1-12. [CrossRef]
[19] Vlizlo VV (Ed.). Laboratorni metody doslidzhen u biolohii, tvarynnytstvi ta veterynarnii medytsyni. Lviv: Spolom; 2012.
[20] Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259): 680-685. [CrossRef]