Erdem YEŞİLADA, Etil GÜZELMERİÇ, Cansel ÇELİK, Nisa Beril ŞEN, Mehmet Ali OÇKUN

Quali/quantitative research on herbal supplements containing black elder (Sambucus nigra L.) fruits

Black elder (Sambucus nigra L.), rich in phenolics, is used as antiviral, especially to treat common cold andflu. The flower of black elder has been approved by German Commission E and ESCOP for its mentioned usage, and inseveral pharmacopoeias, its qualification parameters were described. However, its fruits with a similar use as theflowers have not been considered by the pharmacopoeias. Even so, the number of black elder fruit supplementsmarketing with different brands is increasing steadily. This study aimed to evaluate the availability and concentrationsof marker components (chlorogenic acid, rutin, and isoquercitrin) in herbal supplements containing black elder fruitscomparatively with the reference plant material by high-performance thin-layer chromatography (HPTLC) and avalidated high-performance liquid chromatography (HPLC) techniques. Additionally, the total anthocyanin amountwas calculated by spectrophotometry in these samples, and the results were expressed as equivalents of cyanidin-3-Oglucoside(C3G). HPTLC fingerprints showed that most of the marketed products did not contain rutin, chlorogenicacid and isoquercitrin. Moreover, their fingerprint comparison with the reference was found to be different. The highestchlorogenic acid, rutin and isoquercitrin amounts were found as 0.742 mg/effervescent tablet (E3), 1.445 mg/capsule(C1), and 0.082 mg/capsule (C1), respectively by HPLC. According to the total anthocyanin content, C1 was determinedto have the highest anthocyanin content as 8.705 mg C3G/capsule, whereas E6 was found to have the lowestanthocyanin content as 0.024 mg C3G/effervescent tablet. Consequently, there is an urgent need to establishstandardization parameters for black elder fruits.

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[1] Clayville LR. Influenza update: a review of currently available vaccines. P&T. 2011; 36(10): 659-684.
[2] Kamali A, Holodniy M. Influenza treatment and prophylaxis with neuraminidase inhibitors: a review. Infect Drug Resist. 2013; 6: 187-198. [CrossRef]
[3] European Pharmacopoeia (Ph. Eur.), Seventh ed., Council of Europe, Strasbourg, France, 2010.
[4] Porter RS, Bode RF. A review of the antiviral properties of black elder (Sambucus nigra L.) products. Phytother Res. 2017; 31(4): 533-554. [CrossRef]
[5] Młynarczyk K, Walkowiak-Tomczak D, Łysiak GP. Bioactive properties of Sambucus nigra L. as a functional ingredient for food and pharmaceutical industry. J Funct Foods. 2018; 40: 377-390. [CrossRef]
[6] Roschek Jr B, Fink RC, McMichael MD, Li D, Alberte RS. Elderberry flavonoids bind to and prevent H1N1 infection in vitro. Phytochemistry. 2009; 70(10): 1255-1261. [CrossRef]
[7] Hawkins J, Baker C, Cherry L, Dunne E. Black elderberry (Sambucus nigra) supplementation effectively treats upper respiratory symptoms: A meta-analysis of randomised, controlled clinical trials. Complement Ther Med. 2019; 42: 361-365 [CrossRef]
[8] World Health Organization (WHO) Monographs on Selected Medicinal Plants, Geneva, 1999.
[9] The Complete German Commission E Monographs, American Botanical Council, Texas, 1998.
[10] European Scientific Cooperative on Phytotherapy (ESCOP) Monographs, Second ed., Thieme, New York, 2003.
[11] Maleš Ž, Plazibat M, Bilušić Vundać V, Žuntar I. Qualitative and quantitative analysis of flavonoids of the strawberry tree-Arbutus unedo L. (Ericaceae). Acta Pharm. 2006; 56(2): 245-250.
[12] Łata E, Fulczyk A, Kowalska T, Sajewicz M. Thin-layer chromatographic method of screening the anthocyanes containing alimentary products and precautions taken at the method development step. J Chromatogr A. 2017; 1530, 211-218. [CrossRef]
[13] ICH Harmonised Tripartite Guideline Validation of Analytical Procedures: Text and Methodology Q2 (R1). https://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__G uideline.pdf (accessed on 8 August 2019).
[14] Sidor A, Gramza-Michałowska A. Advanced research on the antioxidant and health benefit of elderberry (Sambucus nigra) in food-a review. J Funct Foods. 2015; 18: 941-958. [CrossRef]
[15] Vrchotová N, Dadáková E, Matějíček A, Tříska J, Kaplan J. Effect of variety on content of bioactive phenolic compounds in common elder (Sambucus nigra L.). Nat Prod Res. 2017; 31(6): 700-703. [CrossRef]
[16] Nagl M, Eder R, Wendelin S, Reich G, Sontag G. Qualitative and quantitative analysis of phenolic constituents in elderberry juices. Nutrition Vienna 2006; 30(10): 409-415.
[17] Mudge E, Applequist WL, Finley J, Lister P, Townesmith AK., Walker KM., Brown, PN. Variation of select flavonols and chlorogenic acid content of elderberry collected throughout the Eastern United States. J Food Compos Anal. 2016; 47: 52-59. [CrossRef]
[18] Dawidowicz AL., Wianowska D, Baraniak B. The antioxidant properties of alcoholic extracts from Sambucus nigra L. (antioxidant properties of extracts). LWT-Food Sci. Technol. 2006; 39(3): 308-315. [CrossRef]
[19] Viapiana A, Wesolowski M. HPLC fingerprint combined with quantitation of phenolic compounds and chemometrics as an efficient strategy for quality consistency evaluation of Sambucus nigra berries. Nat. Prod. Commun. 2016; 11(10): 1934578X1601101009. [CrossRef]
[20] Csorba V, Toth M, Laszlo A, Kardos L, Kovacs S. Cultivar and year effects on the chemical composition of elderberry (Sambucus nigra L.) fruits. Notulae Botanicae Horti Agrobotanici Cluj-Napoca. 2020; 48(2): 770-782. [CrossRef]
[21] Tosun M, Ercisli S, Akbulut M. Physicochemical characteristics of some wild grown European elderberry (Sambucus nigra L.) genotypes. Pharmacogn Mag. 2009; 5(20): 320-323. [CrossRef]
[22] Anton AM, Pintea A, Rugină D, Diaconeasa Z, Hanganu D, Vlase L, et al. Preliminary studies on the chemical characterisation and antioxidant capacity of polyphenols from Sambucus sp. Dig J Nanomater Bıos. 2013; 8: 973-980.
[23] Kaack K, Austed T. Interaction of vitamin C and flavonoids in elderberry (Sambucus nigra L.) during juice processing. Plant Foods Hum Nutr. 1998; 52(3): 187-198.
[24] Ochmian I, Oszmiański J, Skupień K. Chemical composition, phenolics, and firmness of small black fruits. Journal of Applied Botany and Food Quality. 2009; 83: 64-69.
[25] Veberic R, Jakopič J, Stampar F, Schmitzer V. European elderberry (Sambucus nigra L.) rich in sugars, organic acids, anthocyanins and selected polyphenols. Food Chem. 2009; 114: 511-515. [CrossRef]
[26] Kaack K, Fretté XC, Christensen LP, Landbo A-K, Meyer AS. Selection of elderberry (Sambucus nigra L.) genotypes best suited for the preparation of juice. Eur Food Res Technol. 2008; 226(4): 843-855.
[27] Duymuş HG, Göger F, Başer KH. In vitro antioxidant properties and anthocyanin compositions of elderberry extracts. Food Chem. 2014; 155: 112-119. [CrossRef]
[28] Młynarczyk K, Walkowiak-Tomczak D, Lysiak G. Bioactive properties of Sambucus nigra L. As a functional ingredient for food and pharmaceutical industry. Journal of Functional Foods. 2018; 40: 377-390. [CrossRef]
[29] Młynarczyk K, Walkowiak-Tomczak D, Staniek H, Kidoń M, Lysiak G. The content of selected minerals, bioactive compounds, and the antioxidant properties of the flowers and fruit of selected cultivars and wildly growing plants of Sambucus nigra L. Molecules. 2020; 25: 876. [CrossRef]
[30] Vlachojannis C, Zimmermann BF, Chrubasik-Hausmann S. Quantification of anthocyanins in elderberry and chokeberry dietary supplements. Phytother Res. 2015; 29(4): 561-565. [CrossRef]
[31] Krawitz C, Mraheil MA, Stein M, Imirzalioglu C, Domann E, Pleschka S, Hain T. Inhibitory activity of a standardised elderberry liquid extract against clinically-relevant human respiratory bacterial pathogens and influenza A and B viruses. BMC Compl Alternative Med. 2011; 11:16. [CrossRef]
[32] Tiralongo E, Wee SS, Lea RA. Elderberry supplementation reduces cold duration and symptoms in air-travellers: A randomised, double-blind placebo-controlled clinical trial. Nutrients. 2016; 8(4): 182. [CrossRef]
[33] Lee J, Durst RW, Wrolstad RE. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: collaborative study. J AOAC Int. 2005; 88(5): 1269-1278.