Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nın Spesifik Olmayan Savunma Mekanizması Üzerine Geofit Bitki Ekstraktının İmmunostimulant Etkisi
Bu çalışmada amacımız, gökkuşağı alabalığının spesifik olmayan savunma mekanizması üzerine geofit bitki ektraktının immunostimulant etkisinin belirlenmesidir. Bu amaçla geofit bir bitki olan M. comosum Muğla bölgesinden toplandı ve etanolde ekstrakte edildi. Daha sonra farklı dozlarında (0,5 mg/balık ve 2,0 mg/balık) balıklara intraperitonal olarak uygulandı. Balıklar ortalama 140 g ağırlığa sahipti. Deneme süresince uygulamayı takiben 1, 7, 14, 21, 28. günlerde herbir gruptaki balıktan kan ve serum örnekleri alınarak farklı parametreler (hematokrit yüzdesi, nitroblue tetrazolium (NBT) pozitif nötrofil sayısı, toplam lökosit sayısı, beyaz kan hücrelerinin yüzdesi ve serum lizozim aktivitesi) açısından incelendi. Bu çalışmanın sonucunda NBT pozitif nötrofillerin sayısı, monosit ve nötrofil yüzdeleri ve toplam lökosit sayısı 0,5 mg/balık dozunda injeksiyon yapılan grupta kontrol grubuna kıyasla artış gösterdiği (P
Immunostimulant Effects of Geophyte Plant Extract on Non-specific Defence Mechanisms of Rainbow Trout (Oncorhynchus mykiss)
The aim of this study was to determine the immunostimulant effects of geophyteplant extract on non-specific defence mechanisms of rainbow trout(Oncorhynchus mykiss). For this purpose, Muscari comosum was collected inMuğla region and extracted in ethanol. Then, The plant extract applied into fishby intraperitonal injection in two different concentrations (0.5mg/ fish and 2.0mg/fish). The average fish weight was 140 g. Following the injection on the 1st,7th, 14th, 21st, 28th days the blood and serum samples were collected from fishin each group and examined for various parameters including percentage ofhematocrit, the counts of nitroblue tetrazolium (NBT) positive neutrophils, totalleukocyte counts, percentage of white blood cells and serum lysozyme activity.The results indicated that the counts of NBT (+) neutrophils, percentage ofmonocyte and neutophil and total leukocyte counts increased in the group withinjected 0.5mg plant extract /fish compared to control group (P
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- Aly SM, Mohamed MF. 2010. Echinacea purpurea and
Allium sativum as immunostimulants in fish culture
using Nile tilapia (Oreochromis niloticus). J Anim
Physiol Anim Nutr (Berl). 94 (5), e31-e39
doi: 10.1111/j.1439-0396.2009.00971.x
- Anderson DP, Moritomo T, Grooth RD. 1992.
Neutrophile, glass-adherent, nitroblue tetrazolium
assay gives early indication of immunization
effectiveness in rainbow trout. Vet.
Immunol.Immunopathol.30(4):419-429.
doi:10.1016/0165-2427(92)90110-C
- Awad E, Awaad A. 2017. Role of medicinal plants on
growth performance and immune status in fish. Fish
Shellfish Immunol. 67(1):40-54.
doi: 10.1016/j.fsi.2017.05.034
- Awad E, Austin D, Lyndon AR. 2013. Effect of black
cumin seed oil (Nigella sativa) and nettle extract
(Quercetin) on enhancement of immunity in rainbow
trout, Oncorhynchus mykiss (Walbaum). Aquaculture
388-391(1):193–197
doi: 10.1016/j.aquaculture.2013.01.008
- Baba E, Acar Ü, Öntaş C, Kesbiç OS, Yilmaz S. 2016. The
use of Avena sativa extract against Aeromonas
hydrophila and its effect on growth performance,
hematological and immunological parameters in
common carp (Cyprinus carpio). Ital. J. Anim. Sci. 15
(2): 325-333.
doi: 10.1080/1828051X.2016.1185977
- Baba E, Uluköy G, Mammadov R. 2014. Effects of
Muscari comosum Extract on Nonspecific Immune
Parameters in Gilthead Seabream, Sparus aurata (L.
1758). J World Aquacult. Soc. 45 (2): 173-182
doi: 10.1111/jwas.12100
- Christybapita D, Divyagnaneswari M, Michael RD. 2007.
Oral administration of Eclipta alba leaf aqueous
extract enhances the non-specific immune responses
and disease resistance of Oreochromis mossambicus.
Fish Shellfish Immunol. 23(4):840-852
doi: 10.1016/j.fsi.2007.03.010
- Deivasigamani B, Subramanian V. 2016. Applications of
Immunostimulants in Aquaculture: A Review. Int. J.
Curr. Microbiol. App. Sci. 5(9): 447-453.
doi: 10.20546/ijcmas.2016.509.048
- Dügenci SK, Arda N, Candan A. 2003. Some medicinal
plants as immunostimulant for fish. J
Ethnopharmacol.88 (1), 99-106
doi: 10.1016/S0378-8741(03)00182-X
- Engstad RE, Robertsen B, Frivold E. 1992. Yeast glucan
induces increase in lysozyme and complementmediated haemolytic activity in Atlantic salmon blood.
Fish Shellfish Immunol. 2 (4): 287-297
doi: 10.1016/S1050-4648(06)80033-1
- Immanue G, Uma RP, Iyapparaj P, Citarasu T, Punitha
Peter SM, Michael Babu M, Palavesam A. 2009.
Dietary medicinal plant extracts improve growth,
immune activity and survival of tilapia Oreochromis
mossambicus. J Fish Biol 74 (7): 1462-1475
doi: 10.1111/j.1095-8649.2009.02212.x
- Lee TH, Qiu F, Waller GR, Chou CH. 2000. Three new
flavonol galloylglycosides from leaves of Acacia
confusa. J. Nat. Prod. 63(5): 710-712
doi: 10.1021/np990482w
- Logambal SM, Venkatalakshmi S, Michael RD. 2000.
Immunostimulatory effect of leaf extract of Ocimum
sanctum Linn. in Oreochromis mossambicus (Peters).
Hydrobiologia, 430 (1-3):113-120.
doi: 10.1023/A:1004029332114
- Loizzo MR, Tundis R, Menichini F. 2010. Chelating,
antioxidant and hypoglycaemic potential of Muscari
comosum (L.) Mill. bulb extracts. Int J Food Sci Nutr.
61(8):780-791.
doi: 10.3109/09637486.2010.482521
- Magnadottir B. 2006. Innate immunity of fish (Overview).
Fish Shellfish Immunol. 20 (2): 137-151.
doi: 10.1016/j.fsi.2004.09.006
- Mehana EE, Rahmani AH, Aly SM. 2015.
Immunostimulants and Fish Culture: An Overview.
Annu Res Rev Biol. 5 (6):477-489
doi: 10.9734/ARRB/2015/9558
- Nasrabadi, M, Halimi M, Nadaf M. 2013. Phytochemical
screening and chemical composition of extract of
Muscari neglectum. Middle-East J Sci Res 14(4):566–
569.
doi: 10.5829/idosi.mejsr.2013.14.4.71191
- Pieroni, A, Janiak V, Durr CM, Ludeke S, Trachsel E,
Heinrich M. 2002. In vitro antioxidant activity of noncultivated vegetables of ethnic Albanians in southern
Italy. Phytother. Res. 16 (5):467–473.
doi: 10.1002/ptr.1243
- Plumb, J. A., and Hanson, LA. 2011. Health Maintenance
and Principal Microbial Diseases of Cultured Fishes.
Hoboken, NJ: Wiley-Blackwell.
doi: 10.1002/9780470958353
- Pratheepa V, Ramesh S, Sukumaran N. 2010.
Immunomodulatory effect of Aegle marmelos leaf
extract on freshwater fish Cyprinus carpio infected by
bacterial pathogen Aeromonas hydrophila. Pharm.
Biol.48 (11): 1224-1239
doi: 10.3109/13880201003713598
- Schaperclaus W, Kulow H, Schreckenbach
K. (1991) Hematological and serological technique.
In: Fish Disease, Vol. 1, 2nd edn, ed.
by V.S.Kothekar), pp. 71–108. Gulab primlani,
Oxonian Press, New Delhi, India.
- Steinhagen D, Kruse P, Körting W. 1990. Some
heamatological observations on carp, Cyprinus carpio
L. experimentally infected with Trypanoplasma
borreli Laveron. J Fish Dis. 13(2) :157–162
doi: 10.1111/j.1365-2761.1990.tb00768.x
- Sudhakaran DS, Srirekha P, Devasree LD, Premsingh S,
Michael RD. 2006. Immunostimulatory effect of
Tinospora cordifolia Miers leaf extract in
Oreochromis mossambicus. Indian J Exp Biol. 44
(9):726-732
- Sharma A, Deo AD, Riteshkumar ST, Chanu TI, Das A.
2010. Effect of Withania somnifera (L. Dunal) root as
a feed additive on immunological parameters and
disease resistance to Aeromonas hydrophila in Labeo
rohita (Hamilton) fingerlings. Fish Shellfish
Immunol. 29 (3):508-512
doi: 10.1016/j.fsi.2010.05.005
- Tanker M, Tanker N. 1991. Farmakognozi Cilt 1.
Ankara Universitesi, Eczacılık Fakültesi
yayını, No:66, Ankara. (In Turkish) [cited 2017
November 11] Available from
http://kitaplar.ankara.edu.tr/dosyalar/pdf/313.pdf
- Villa F, Borgonovo G, Cappitelli F, Giussani B, Bassoli
A. 2012. Sub-lethal concentrations of Muscari
comosum bulb extract suppress adhesion and induce
detachment of sessile yeast cells. Biofoling
28(10):1107–1117.
doi: 10.1080/08927014.2012.734811