GÜLŞEN ULUKÖY, AYŞEGÜL KUBİLAY, Behire Işıl DİDİNEN, SEÇİL METİN, SONER ALTUN, ÖZNUR DİLER, RAMAZAN MAMMADOV, Arife DULLUÇ

Immunostimulant Effects of Geophyte Plant Extract on Non-specific Defence Mechanisms of Rainbow Trout (Oncorhynchus mykiss)

The aim of this study was to determine the immunostimulant effects of geophyte plant extract on non-specific defence mechanisms of rainbow trout (Oncorhynchus mykiss). For this purpose, Muscari comosum was collected in Muğla region and extracted in ethanol. Then, The plant extract applied into fish by intraperitonal injection in two different concentrations (0.5mg/ fish and 2.0 mg/fish). The average fish weight was 140 g. Following the injection on the 1st, 7th, 14th, 21st, 28th days the blood and serum samples were collected from fish in each group and examined for various parameters including percentage of hematocrit, the counts of nitroblue tetrazolium (NBT) positive neutrophils, total leukocyte counts, percentage of white blood cells and serum lysozyme activity. The results indicated that the counts of NBT (+) neutrophils, percentage of monocyte and neutophil and total leukocyte counts increased in the group with injected 0.5mg plant extract /fish compared to control group (P

Gökkuşağı Alabalığı (Oncorhynchus mykiss)’nın Spesifik Olmayan Savunma Mekanizması Üzerine Geofit Bitki Ekstraktının İmmunostimulant Etkisi

Bu çalışmada amacımız, gökkuşağı alabalığının spesifik olmayan savunma mekanizması üzerine geofit bitki ektraktının immunostimulant etkisinin belirlenmesidir. Bu amaçla geofit bir bitki olan M. comosum Muğla bölgesinden toplandı ve etanolde ekstrakte edildi. Daha sonra farklı dozlarında (0,5 mg/balık ve 2,0 mg/balık) balıklara intraperitonal olarak uygulandı. Balıklar ortalama 140 g ağırlığa sahipti. Deneme süresince uygulamayı takiben 1, 7, 14, 21, 28. günlerde herbir gruptaki balıktan kan ve serum örnekleri alınarak farklı parametreler (hematokrit yüzdesi, nitroblue tetrazolium (NBT) pozitif nötrofil sayısı, toplam lökosit sayısı, beyaz kan hücrelerinin yüzdesi ve serum lizozim aktivitesi) açısından incelendi. Bu çalışmanın sonucunda NBT pozitif nötrofillerin sayısı, monosit ve nötrofil yüzdeleri ve toplam lökosit sayısı 0,5 mg/balık dozunda injeksiyon yapılan grupta kontrol grubuna kıyasla artış gösterdiği (P

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