Farklı Protein Kaynaklarının Çuka Mersini, Acipenser ruthenus Linnaeus 1758, Juvenillerinde Proteaz Aktivitesi Üzerine Etkileri
Bu çalışmada, çuka balığı, Acipenser ruthenus, larvaları yumurtadan çıkış anından itibaren 29 gün boyunca standart besleme protokolü ile beslenmiştir. Karma yeme geçiş yapılan 29. gündeki proteolitik aktivite ve farklı protein kaynaklarının proteaz aktivite üzerine inhibisyon etkileri ölçülmüştür. Çalışmadan elde edilen bilgiler ışığında, çuka balığı juvenillerinin karma yemlerinde protein kaynağı olarak soya protein konsantresi (%38,57±10,44) ve mısır gluten (%43,07±1,72) kullanmanın, diğer yem hammaddeleri ile karşılaştırıldığında, proteolitik enzim aktivitesini istatistiki açıdan daha olumlu etki yaptığı görülmüştür (P0,05). En yüksek inhibe edici etki ise, buğday ile pirinç kepeğinin 3:1 oranda kullanılması ile oluşmuştur (%82,39±7,23).
Effects of Different Protein Sources on Proteolotic Activity in Juveniles of Sterlet, Acipenser ruthenus Linnaeus 1758
In this trial, Sterlet sturgeon, Acipenser ruthenus, larvae were fed by a standart feeding protocol from hatcing until 29th day, and the proteolytic activity and the inhibition effect of different portein sources on protease activity were also measured. The data obtained from study showed that using soybean protein consantrate (38.57±10.44%) and corn gluten (43.07±1.72%) as protein source in mixed feeds of dulcite juvenile strains compared to other feed raw materials, was found to have a more positive effect on proteolytic enzyme activity in terms of statistics (P0.05). The highest inhibitory effect was obtained by using 3: 1 ratio of wheat and brass bran (82.39±7.23%).
___
- Alarcon FJ, Moyano FJ, Diaz M, Fernandez-Diaz C,
Yufera M. 1999. Optimization of the protein fraction
of microcapsules used in feeding of marine fish larvae
using in vitro digestibility techniques. Aquac Nutr.
5(2):107-113.
doi: 10.1046/j.1365-2095.1999.00093.x
- Alptekin C, Gökçek K. 2016. Balık yemlerinde kullanılan
farklı protein kaynaklarının Sibirya mersini,
Acipenser baerii Brandt 1869, juvenillerinin proteaz
aktivitesi üzerine etkileri. Yunus Araştırma Bülteni.
16(3):201-207. [in Turkish]
doi: 10.17683/yunus.32866
- Babaei SS, Kenari AA, Nazari R, Gisbert E. 2011.
Developmental changes of digestive enzymes in
Persian Sturgeon (Acipenser percius) during larval
ontogeny. Aquaculture. 318(1-2):138-144.
doi: 10.1016/j.aquaculture.2011.04.032
- Bradford MM. 1976. A rapid sensitive method for the
quantification of microgram quantities of protein
utilizing the principle of protein-dry binding. Anal
Biochem. 72:248-254.
- Furne M, Hidalgo MC, Lopez A, Garcia-Gallego M,
Morales AE, Domezain A, Domezaine J, Sanz A.
2005. Digestive enzyme activities in Adriatic sturgeon
Acipenser naccarii and rainbow trout
Oncorhynchus mykiss. A comperative study.
Aquaculture. 250(1-2):391-398.
doi: 10.1016/j.aquaculture.2005.05.017
- Garcia-Carreno FL. 1996. Proteinase inhibitors. Trends
Food Sci Technol. 7(6):197-204.
doi: 10.1016/0924-2244(96)10023-6
- Gawlicka A, Herold MA, Barrows FT, de le Noue J, Hung
SSO. 2002. Effect of dieatary lipids on growth, fatty
acid composition, intestinal absorbtion and hepatic
storage in white sturgeon (Acipenser transmontanus)
larvae. J Appl Icthyol. 18(4-6):673-681.
doi: 10.1046/j.1439-0426.2002.00371.x
- Gökçek K, Szabo T, Alptekin C, Kurt R, Töre Y, Urbanyi
B. 2016. The effect of feeding regime and inhibitory
of different feed ingredients on proteases of
Russian sturgeon, Acipenser gueldenstaedtii
Brandt&Ratzenburg, 1833, at early life stages.
Turkish J of Fisheries and Aquatic Sciences.
16(4): 1025-1029.
doi: 10.4194/1303-2712-v16_4_29
- Kotzamanis YP, Vatsos IN, Cremer M, Illia V, Pyrenis G,
Zonaras V, Buentello A, Van Eys J, 2012. Effects of
high dietary inclusion of soybean meal and Navita
3010 soy supplemented with taurine on growth
performance and muscle composition of European
seabass (Dicentrarchus labrax). HCMR, Greece.
(Technical Report)
- Napora-Rutkowski L, Kamaszewski M, Bielawski W,
Ostaszewska T, Wegner A. 2009. Effects of starter
diets on pancreatic enzyme activity in juvenile starlet
(Acipenser ruthenus). The Israeli J AquacultureBamidgeh, 61 (2): 143-150.
- Noori F, Gilbert VS, Sorgeloos P. 2012. Preliminary study
on the activity of protease enzymes in Persian
sturgeon (Acipenser persicus Borodin, 1897) larvae in
response to different diets: effects on growth and
survival. Aquac Res. 43(2):198-207.
doi: 10.1111/j.1365-2109.2011.02816.x
- Sanz A, Llorente JL, Furne M, Ostos-Garrido V, Carmona
R, Domezain A, Hidalgo MC. 2011. Digestive
enzymes during ontogeny of the sturgeon Acipenser
naccarii: intestine and pancreas development.
J Appl Ichthyol. 27(5):1139-1146.
doi: 10.1111/j.1439-0426.2011.01864.x
- Töre Y, Gökçek K, Alptekin C, Szabo T, Urbanyi B. 2014.
Turna, Esox lucius Linneaus 1758, balığı larvalarının
proteaz aktivitesi üzerine ticari yem hammaddelerinin
inhibisyon etkilerinin tespiti. Doğu Anadolu 5. Su
Ürünleri Sempozyumu; Elazığ, Türkiye.
- Walter HE. 1984. Proteinases: methods with hemoglobin,
casein and azocoll as sub-strates, In: Methods of
Enzymatic Analysis, Vol. 5. Weinheim: Verlag
Chemie Press. s. 270-277.
- Willot P, Sabeau L, Gessner J, Arlati G, Bronzi P, Gulyas
T, Berni P. 2001. Sturgeon farming in Western
Europe: recent developments and perspectives. Aquat
Living Resourc. 14(6):367-374.
doi: 10.1016/S0990-7440(01)01136-6