Hüseyin SEVGİLİ, Soner SEZEN, MAhir KANYILMAZ, Özgür AKTAŞ, Faruk PAK

Dietary Protein Requirements of Zebrafish (Dania rerio)

Zebrafish (Danio rerio) with an initial weight of 88.61±0.82 mg were fed eight isoenergetic diets containing dietary protein levels ranging from 20 to 55 % by 5 % increments. Each diet was feed in triplicate of fish for 6 weeks. Specific growth rates (SGR) at week 2 and 4 were quadratically affected by the treatments but this trend disappeared at the end of the experiment. Dietary protein levels linearly reduced the values of daily feed intake, feed conversion ratio and protein efficiency rate. The whole body dry matter, ash and lipid concentrations linearly decreased with dietary protein levels whereas whole body protein was quadratically affected. The second order polynomial and two break point linear models (TBPLM) were used to estimate dietary protein requirements. The later model generated lower residual sum of squares when SGRWeek4 and SGRFinal values were used as a response. Minimum dietary protein requirements for SGRWeek4 and SGRFinal were estimated by the TBPLM as 27.69 and 28.93 % respectively. Briefly, results of the study suggest a minimum dietary protein requirement of zebrafish is about 29 % for maximum growth rate.

Zebra Balığının (Dania rerio) Diyetsel Protein Gereksinimi

Ortalama başlangıç ağırlığı 88,61±0,82 mg olan zebra balıkları (Danio rerio) protein düzeyi % 20-55 arasında değişen sekiz adet izoenerjitik yemle beslenmiştir. Her bir deneme yemi üç tekrarlı olarak 6 hafta boyunca balıklara verilmiştir. Spesifik büyüme oranı (SGR) 2. ve 4. haftalarda kuadratik olarak etkilenirken, bu eğilim deneme sonunda kaybolmuştur. Protein düzeyleri arttıkça yem tüketimi, yemden yararlanma oranı ve protein etkinlik oranı doğrusal olarak düşmüştür. Tüm vücut kuru madde, kül ve lipit konsantrasyonları diyetsel protein düzeyinin artışı ile doğrusal olarak düşmüş, vücut protein düzeyi ise kuadratik olarak etkilenmiştir. Diyetsel protein gereksinimlerini tahmin etmek için, ikinci derece regresyon ve iki kırıklı linear model (İKLM) kullanılmıştır. 4. hafta ve deneme sonu SGR değerleri kullanıldığında İKLM daha düşük kalıntı kareler toplamı vermiştir. 4. hafta ve final SGR oranlarına göre, İKLM minimum protein gereksinimlerini sırasıyla, % 27,69 ve % 28,93 olarak tahmin etmiştir. Kısaca, çalışma bulguları zebra balıklarının maksimum büyüme için minimum protein gereksinimlerinin yaklaşık % 29 olduğunu göstermektedir.

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Akpιnar Z, Sevgili H, Özgen T, Demir A, Emre Y. 2012. Dietary protein requirement of juvenile shi drum, Umbrina cirrosa (L.). Aquacult Res. 43:421-429. doi: 10.1111/j.1365-2109.2011.02845.x

AOAC. 1990. Official Methods of Analysis, 15 ed., Association of Official Analytical Chemists, Arlington, VA, p.684

Biga PR, Goetz FW. 2006. Zebrafish and giant danio as models for muscle growth: determinate vs. indeterminate growth as determined by morphometric analysis. Am J Physiol Regul Integr Comp Physiol. 291:R1327-R1337. doi: 10.1152/ajpregu.00905.2005

Eaton RC, Farley RD. 1974. Growth and the reduction of depensation of zebrafish, Brachydanio rerio, reared in the laboratory. Copeia. 1974(1):204-209. doi: 10.2307/1443024

El‐ Dakar AY, Shalaby SM, Saoud IP. 2011. Dietary protein requirement of juvenile marbled spinefoot rabbitfish Siganus rivulatus. Aquacult Res. 42:1050- 1055. doi: 10.1111/j.1365-2109.2010.02694.x

Fernandes H, Peres H, Carvalho AP. 2016. Dietary protein requirement during juvenile growth of Zebrafish (Danio rerio). Zebrafish. 13(6):548-555. doi: 10.1089/zeb.2016.1303

Gurure R, Moccia R, Atkinson J. 1995. Optimal protein requirements of young Arctic charr (Salvelinus alpinus) fed practical diets. Aquacult Nutr. 1:227-234. doi: 10.1111/j.1365-2095.1995.tb00048.x

Hernandez-Llamas A. 2009. Conventional and alternative dose–response models to estimate nutrient requirements of aquaculture species. Aquaculture. 292:207-213. doi: 10.1016/j.aquaculture.2009.04.014

Kim S-S, Lee K-J. 2009. Dietary protein requirement of juvenile tiger puffer (Takifugu rubripes). Aquaculture. 287(1-2):219-222. doi: 10.1016/j.aquaculture.2008.10.021

Klatt SF, von Danwitz A, Hasler M, Susenbeth A. 2016. Determination of the lower and upper critical concentration of Methionine+ Cystine in diets of juvenile turbot (Psetta maxima). Aquaculture. 452:12- 23. doi: 10.1016/j.aquaculture.2015.10.015

Lawrence C. 2007. The husbandry of zebrafish (Danio rerio): a review. Aquaculture. 269(1-4):1-20. doi: 10.1016/j.aquaculture.2007.04.077

Markovich ML, Rizzuto NV, Brown PB. 2007. Diet affects spawning in zebrafish. Zebrafish. 4(1):69-74. doi: 10.1089/zeb.2006.9993

NRC. 2011. Nutrient requirements of fish and shrimp. National Research Council of the National Academies, Washington DC.

O'Brine TM, Vrtělová J, Snellgrove DL, Davies SJ, Sloman KA. 2015. Growth, oxygen consumption, and behavioral responses of Danio rerio to variation in dietary protein and lipid levels. Zebrafish 12(4):296- 304. doi: 10.1089/zeb.2014.1008

Robison BD, Drewa RE, Murdoch GK, Powell M, Rodnick KJ, Settles M, Stone D, Churchill A, Hill RA, Papasani MR, Lewis SS, Hardy RW. 2008. Sexual dimorphism in hepatic gene expression and the response to dietary carbohydrate manipulation in the zebrafish (Danio rerio). Comp Biochem Physiol Part D Genomics Proteomics. 3(2):141-154. doi: 10.1016/j.cbd.2008.01.001

Shearer K. 2000. Experimental design, statistical analysis and modelling of dietary nutrient requirement studies for fish: a critical review. Aquacult Nutr. 6(2):91-102. doi: 10.1046/j.1365-2095.2000.00134.x

Shyong W-J, Huang C-H, Chen H-C. 1998. Effects of dietary protein concentration on growth and muscle composition of juvenile Zacco barbata. Aquaculture. 167:35-42. doi: 10.1016/S0044-8486(98)00313-5

Siccardi III AJ, Garris HW, Jones WT, Moseley DB, D'Abramo LR, Watts SA. 2009. Growth and survival of zebrafish (Danio rerio) fed different commercial and laboratory diets. Zebrafish. 6(3):275-280. doi: 10.1089/zeb.2008.0553

Smith Jr DL, Barry RJ, Powell ML, Nagy TR, D'Abramo L, Watts SA. 2013. Dietary protein source influence on body size and composition in growing zebrafish. Zebrafish. 10(3):439-446. doi: 10.1089/zeb.2012.0864

Ulloa PE, Iturra P, Neira R, Araneda C. 2011. Zebrafish as a model organism for nutrition and growth: towards comparative studies of nutritional genomics applied to aquacultured fishes. Rev Fish Biol Fisheries 21(4):649-666. doi: 10.1007/s11160-011-9203-0

Ulloa PE, Medrano JF, Feijoo CG. 2014. Zebrafish as animal model for aquaculture nutrition research. Front Genet. 5:313. doi: 10.3389/fgene.2014.00313

Yossa R, Sarker PK, Mock DM, Vandenberg GW. 2014. Dietary biotin requirement for growth of juvenile zebrafish Danio rerio (Hamilton-Buchanan). Aquacult Res. 45(11):1787-1797. doi: 10.1111/are.12124