Doğu Karadeniz Bölgesinde Amfibi Patojeni Batrachochytrium dendrobatidis’in Potansiyel Dağılımı

Amfibi patojeni Batrachochytrium dendrobatidis Anadolu’da rapor edilmesine rağmen, hem coğrafik dağılımı hem de Türkiye amfibileri üzerindeki etkisi hala belirsizdir. Bu çalışmada, Doğu Karadeniz Bölgesindeki beş sulak alandan on farklı amfibi türlerine (Pelodytes caucasicus, Rana dalmatina, Rana macrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi Pelophylax ridibundus, Ommatotriton ophryticus, Mertensiella caucasicus) ait 62 ergin birey toplandı. Kantitatif Real-time PCR tekniği ile tüm bireylerde B. dendrobatidis enfeksiyonlarının prevalansı ve yoğunluğu ile araştırıldı ve Doğu Karadeniz Bölgesi’ndeki 10 amfibi türünden toplanan 62 bireyin 13’ ünde B. dendrobatidis enfeksiyonu varlığı ilk kez rapor edildi. Enfeksiyon yoğunluğu 403,520-534,340 genomik eşdeğerler arasında değiştiği belirlendi. Amfibi türler arasında en yüksek genomik eşdeğer Uzungöl’ deki P. caucasicus (534,280) ve Karagöl'de B. bufo (504,00) saptandı.

Potential Distribution of the Amphibian Pathogen, Batrachochytrium dendrobatidis in the Eastern Black Sea Region of Turkey

Although Batrachochytrium dendrobatidis, pathogen for amphibians, has beenreported in Anatolia, its geographical distribution, as well as its impact on theamphibians in Turkey, remained obscure. In this study, 62 adult individualsbelonging to ten different species (Pelodytes caucasicus, Rana dalmatina, Ranamacrocnemis, Bufo bufo, Bufo verrucosissimus, Bufotes variabilis, Hyla savignyi,Pelophylax ridibundus, Ommatotriton ophryticus, and Mertensiella caucasicus)were collected from five wetland habitats in Eastern Black Sea Region of Turkey.The prevalence and the intensity of B. dendrobatidis infections in all theindividuals were investigated by using quantitative Real-time-PCR technique andthe presence of B. dendrobatidis infection was reported for the first time in 13 ofthe 62 individuals collected from 10 amphibian species from Eastern Black SeaRegion of Turkey. The intensity of B. dendrobatidis infection ranged from403.520 to 534.280 genomic equivalents (GE) was detected. The highest GEbetween amphibian species were determined in P. caucasicus (534.280 GE) inUzungöl (Çaykara-Trabzon) and B. bufo (504.00 GE) in Lake Karagöl (ŞavşatArtvin).

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