Çimen ATAK, Sema ALİKAMANOĞLU, Özge EMİROĞLU, Aytekin RZAKOULİEVA

Stimulation of regeneration by magnetic field in soybean (Glycine max L. Merrill) tissue cultures

Manyetik alanın doku kültürlerindeki etkilerinin belirlenmesi bu konuda yapılacak çalışmalara yardımcı olacaktır. Bu amaçla başlanılan çalışmada J-357 soya çeşidine ait tohumlar kullanılmıştır. Soya tohumları steril koşullarda çimlendirilmiş ve sürgün ucu kültürleri kurulmuştur. Petri kutuları içerisindeki eksplantlar 2.9-4.6 mT’lık manyetik alandan 2.2, 6.6 ve 19.8 saniyelik sürelerde geçirilmiştir. Kontrol ve manyetik alana maruz bırakılan eksplantlardan rejenere olan sürgün sayıları, kök oluşumları, yaş ağırlık ve klorofil miktarları belirlenmiştir. Sürgün oluşumu kontrol grubunda %61.91 iken bu oran tüm manyetik alan uygulamalarında artmış ve 2.2 ve 6.6 saniyelik sürelerde manyetik alana maruz bırakılan eksplantlarda sırasıyla %86.96 ve %74.36’ya yükselmiştir. Yine kök oluşum yüzdesi kontrolde %14.29 iken aynı sürelerde manyetik alandan geçirilenlerde bu oran sırasıyla %26.08 ve %35.90’a yükselmiştir. Rejenere olan bitkilerin yaş ağırlıkları belirlenmiş ve 6.6 sn süre için manyetik alan uygulanan eksplantlardan rejenere olan fidelerde yaş ağırlıkları kontrole göre önemli olarak artmıştır (P< 0.05). Manyetik alanın klorofil miktarları üzerine etkileri belirlenmiş ve 2.2 sn süreyle manyetten geçirilenlerde klorofil a, klorofil b ve toplam klorofil miktarlarında bir artış saptanmıştır.

Soya (Glycine max L. Merrill) doku kültürlerinde rejenerasyonunun manyetik olan tarafından sitimulasyonu

The determination of the effects of magnetic field on tissue cultures will be helpfull for the magnetic field treatments. In this study which was begun with this purpose, the seeds belonging to J 357 soybean variety were used. The soybean seeds were germinated in sterile conditions and cultures were initiated from the shoot tips. The explants in petri dishes were exposed to 2.9-4.6 mT magnetic fields for 2.2, 6.6, and 19.8 seconds periods. Shoot and root formation rate, fresh weights and chlorophyll quantities of regenerated shoots from control and treated explants were determined. While the shoot formation was 61.91% in control group, this rate increased in all magnetic field experiments and this rate raised up to 86.96% and 74.36% respectively in the explants which were exposed to magnetic field at 2.2 and 6.6 seconds periods. Again, while the percentage of root formation in control was 14.29%, this rate raised up to 26.08% and 35.90% respectively in these which were exposed to magnetic field at the same periods. When the fresh weights were determined, the fresh weights of seedlings regenerated from treatment explants from 6.6 seconds significantly increased in accordance with control (P

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