Amangeldy K. BİSSENBAEV, Nazgül A. ALTYBAEVA, Gulderay A. KOLBAEVA

Role of reactive oxygen species and antioxidant enzymes in hormone regulating programmed cell death of wheat aleurone layer

Alevron tabakasındaki reaktif oksijen türleri ve antioksidan enzim üretimi düzenlenmesi ortaya çıkarılmıştır. Alevron tabakasındaki giberilik asidin varlığı reaktif oksijen türlerinin hızlı bir şekilde artışına sebep olmuştur. Giberilik asidin bu etkisi antioksidan enzim miktarındaki maksimum derecedeki düşüş ile ilişkilendirilmiştir. Mevcut çalışma, bu modelde absisik asid miktarındaki artışın ROS üretimini baskıladığını ve bu baskıyı antioksidan enzim sentezlemek suretiyle yaptığını göstermiştir. Alevron tabakasındaki programlı hücre ölümü ve ROS üretimi ile antioksidan enzim aktivitesi arasındaki sıkı ilişki tespit edilmiştir. Elde edilen bilgiler ışığında, ROS ve antioksidan enzimlerinin hormon teşvikli programlı hücre ölümü üzerinde rolü olduğu söylenebilir.

Reaktif oksijen türleri ve antioksidan enzimlerin buğday alevron tabakasındaki hormon düzenlemeli programlı hücre ölümü üzerindeki rolü

Hormonal regulation of reactive oxygen species and antioxidant enzyme production in wheat aleurone layer was revealed. In the presence of gibberellic acid in wheat aleurone layer significant increase of reactive oxygen species was observed. This effect of gibberellic acid was associated with maximal decrease in the antioxidant enzyme activity. The present study showed that in the given model system influence of abscisic acid was directed to the suppression of ROS production through increase in antioxidant enzyme synthesis. Strict correlation between progression of wheat aleurone programmed cell death, ROS production and changes in activity of antioxidant enzymes was revealed. Relying on the obtained data it was suggested that ROS and antioxidant enzymes could play important roles in realization of hormone induced programmed cell death of wheat aleurone layer.

PDF

___

Bawler C, Camp VW, Montagu VM., Inze D. Superoxide dismutase in plants. Critical Reviews in Plant Science. 13:199-218, 1994.
Beauchamp C, Fridovich I. Superoxide dismutase: improved assay and an assay applicable to acrylamide gels. Analytical Biochemistry. 44: 276-287, 1971.
Bethke PC, Schuurink R and Jones RL. Hormonal signaling in cereal aleurone. J. Exp. Bot. 48: 13337-13356, 1997.
Bissenbaev AK, Tairov MM and Bersimbaev RL Role of protein synthesis and Ca2+ in the effect of gibberellic acid on amylase secretion from isolated aleurone layer of wheat seed. Russian J. of Biochem. 57 (12): 1834- 1840, 1992.
Bissenbaev AK, Keniev AM and Bersimbaev RL Effect of gibberellic abscisic acid on apoptosis of wheat aleurone cells. Bulletin of Kazakh National University. 1 (13): 52- 57,2001.
Bissenbaev AK., Keniev AM, Bersimbaev RL Endogenous deoxyribonucleases involved in nuclear DNA degradation of wheat aleurone cells. Journal of Cell and Molecular Biology. 3: 77-81, 2004.
Brenan T, Frenkel C. Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant. Physiol. 59:411-416,1977.
Camp W, Capiau K., Van Montagu M, Siooten L. Enhancement of oxidative stress tolerance in transgenic tobacco plants overproducing Fe-superoxide dismutase in chloroplasts. Plant Physiol. 112: 1703-1714, 2003.
Fath A, Bethke PC, Lonsdale J., Meza-Romero R, Jones R. Programmed cell death in cereal aleurone. Plant Molecular Biology. 44: 255-256, 2000.
Gilchrist PC Programmed cell death in plant disease: The purpose and promise of cellular suicide. Ann. Rev. PhytopathoL, 21: 345-358. 1998.
Jabs T, Dietrich RA, Dangl JL. Initiation of runaway cell death in an Arabidopsis mutant by extracellular superoxide. Science. 273: 1853-1856, 1996.
Jab TI. Reactive oxygen intermediates as mediators of programmed cell death in plant and animals. Biochem. Pharm. 57: 231-245, 1999.
Jiang M, Zhang J. Effect of abscisic acid on active oxygen species, antioxidative defense system and oxidative damage in leaves of maize seedlings. Plant Cell Physiol. 42(11): 1265-1273, 2001.
Levine A, Tenhaken R, Dixon R, Lamb C. H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response. Cell. 79: 583-593, 1994.
Mateo A, Muhlenbock P, Rusterucci C, Chi-Chen Chang C, Miszalski Z, Karpinska B, Parker JE, Mullineaux PM, Karpinski S Lesion stimulating disease 1 is required for acclimation to conditions that promote excess excitation energy. Plant Physiol., 136: 2818-2830, 2004.
Mittler R, Xuqiao F, Cohen M. Post-transcriptional suppression of cytosolic ascorbate peroxidase expression during pathogen-induced programmed cell death in tobacco. The Plant Cell. 10: 461-473, 1998.
Mittler R, Zilinkas BA. Detection of ascorbate peroxidase activity in native gels by inhibition of the ascorbate - dependent reduction of nitroblue tetrazolium. Analytical Biochemistry. 212: 540-546, 1993.
Overmyer K, Tuominen H, Kettunen R, Betz C, Langebartels C, Sandermann H Jr, Kangasjarvi J. Ozone-sensitive Arabidopsis rcdl mutant reveals opposite roles for ethylene and jasmonate signaling pathways in regulating superoxide-dependent cell death. Plant Cell. 12: 1849-1862, 2000.
Shigeoka Sh., Ishikawa T, Tamoi M, Miyagawa Y, Takeda T, Yabuta Y, Yoshimura K. Regulation and function of ascorbate peroxidase isoenzymes. J. Exp.Botany, 53 (372): 1305-1319, 2002.
Sordet O, Khan QA, Plo I, Pourquier P, Urasaki, Y, Yoshida A, Antony S, Kohlhagen G, Solary E, Saparbaev M, Laval J, and Pommier Y. Apoptotic topoisomerase I- DNA complexes induced by staurosporine-mediated oxygen radicals. J. Biol. Chem. 279: 50499-50504, 2004.
Young TE, Gallie DR, and DeMason DA. Ethylene-Mediated Programmed Cell Death during Maize Endosperm Development of Wild-Type and shrunken2 Genotypes. Plant Physiol. 115: 737-751, 1997
Young TE, Gallie DR. Analysis of programmed cell death in wheat endosperm reveals differences in endosperm development between cereals. Plant Mol Biol. 39: 915- 926, 1999