Thulasi G. PILLAI, Das Puthiyapurayil DAMODHARAN

Prevention of radiation-induced chromosome damage in mouse bone marrow by aqueous leaf extract of Chicorium intybus

Bu çalışmada Chicorium intybus'xm (Chicory) sulu yaprak ekstrelerinin, radyasyon sonucu oluşturulan kromozomal 60 hasara karşı radyoprotektif etkileri araştırılmıştır. Erişkin Swiss albino fareler 4Gy Co y-ışınlarına maruz bırakılmıştır. Radyasyon ile uyarılmadan hemen sonra sulu ekstreler 50 ve 100 mg/kg vücut ağırlığı olacak şekilde oral uygulanmıştır. Radyasyon uygulamasından 24 saat sonra farklı tipte anormallikler ve anormal metafaz sayıları tespit edilerek kemik iliğindeki koruma belirlenmiştir. Ekstre uygulanmış grup radyasyon uygulanmış ancak ekstre uygulanmamış grup ile karşılaştırıldığında anormal hücrelerin ve farklı tipte anormalliklerin sayısında önemli bir azalma tespit edilmiştir. Hayvanlara Chicory sıvı yaprak ekstrelerinin uygulanması mikronukleus oluşumunda kayda değer bir azalma sağlamıştır. Ekstreler önemli hidroksil yakalama aktivitesine de sahiptirler. Sudaki yüksek çözünürlükleri ile radyasyon etkisini takiben kromozomal hasarı önleyici ilaç etkileri, kazasal radyasyon uygulamaları ve nükleer kazalarda uygulamada yararlı kılmaktadır.

Chicorium intybus sulu yaprak ekstreleri ile fare kemik iliğinde radyasyon nedenli kromozomal hasarın önlenmesi

The radioprotective effect of aqueous leaf extract of Chicorium intybus (Chicory) against radiation induced60 chromosomal damage was investigated. Adult Swiss albino mice were exposed to 4 Gy Co y-rays. Aqueous extract (50 and 100 mg/kg body wt) was administered orally just after irradiation. Bone marrow protection was evaluated by scoring the different types of individual aberrations and aberrant metaphases, 24hrs after irradiation. Significant reduction in number of aberrant cells and different type of aberrations was observed in treated group compared to irradiated untreated group of animals. The administration of aqueous leaf extract of Chicory to the animals showed significant reduction in micronucleus induction. The extract also possessed significant hydroxyl radical scavenging activity. The effectiveness of the drug to prevent chromosomal damage consequent to irradiation along with its high solubility in water, favor its application, in accidental radiation exposures and nuclear accidents.

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