Arman M. KENIEV, I. Rakhmetkazhy BERSIMBAEV, Amangeldy K. BISENBAEV

Endogenous deoxyribonucleases involved in nuclear DNA degradation of wheat aleurone cells

PAAG jel elektroforez enzim metodlannın kullanılmasıyla ve izole edilmiş çekirdeklerle yapılan deneylerle buğday tanelerinin alevrone tabakasının 3 tür deoksiribonükleazı (A, B, C) ortaya çıkarıldı. Deoksiribonukleazlar Ca2+, Mg2+ ve gibberellik aside duyarlılıklarına göre ayrıldı. B deoksiribonükleazın aktivasyonu için $Ca^{2+} ve Mg^{2+}$ gerekli değilken, A tür deoksiribonükleaz için varlığının gerekli olduğu görüldü ( $Ca^{2+} / Mg^{2+}$ , e bağımlı deoksiribonükleaz). GA'den etkilenen türün (C türü) aktivasyonu sadece $Ca^{2+}$ iyonlarının varlığında gözlendi ( $Ca^{2+}$ e bağımlı deoksiribonükleaz).

Buğday alevrone hücrelerinin nuklear DNA' sının yıkımına katılan endogenous deoksiribonukleazlar

By using PAAG gel electrophoresis enzyme assay methods and on the basis of experiments in isolated nucleus three types of deoxyribonuclease (A,B,C of aleurone layer of wheat seeds were revealed. Deoxyribonucleases were differentiated by their sensitivity to divalent cations and gibberellic acid (GA). It has been shown that for activation of B deoxyribonuclease $Ca^{2+}$ and $Mg^{2+}$ are not required, whereas for A forms of enzyme the presence of cations for activity ( $Ca^{2+} / Mg^{2+}$ dependent deoxyribonucleas) is necessary. Activation of GA- induced form (C form) was observed only in the presence of $Ca^{2+}$ ions ( $Ca^{2+}$ dependent deoxyribonuclease).

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