Characterization of downy mildew isolates of Sclerospora graminicola by using differential cultivars and molecular markers
Hindistan’ın farklı agroklimatik bölgelerinden seçilen 27 pearl millet, Sclerospora graminicola downy mildew izolatlarını analiz etmek için 14 farklı kultivar seti kullanılmıştır. 27 izolat arasında 6 farklı patotip belirlenmiştir. Bu izolatların kullanılan farklı kültüvarların bir kısmını enfekte ettiği ve hastalık insidansının yüzde 0 ila yüzde 98 arasında değiştiği bulunmuştur. Altı S. graminicola patotip ile RAPD ve ISSR markör karşılaştırma analizleri yapılmıştır. Toplam 20 rastgele ve 19 ISSR primerleri 152 ve 297 bantlarını oluşturmuştur, % 73.6 ve % 82.15 polimorfizmlerini açığa çıkarmıştır, 6 patotipi ayırt edebilmiştir. RAPD’de elde edilen maksimum genetik mesafe indeksi patotip-2 ve patotip-3 arasında 0.1312 ve minumum mesafe patotip-1 ve patotip-4 arasında 0.0231’dir. ISSR’da patotip-4 ve patotip-6 arasında 0.1302 maksimum değer, 0.0124 patotip-1 ve patotip-5 arasında minimum mesafe değeri olarak tespit edilmiştir. Grup analiz verilerinden elde edilen UPGMA dendogramlar RAPD için benzerlik katsayısını 0.27 ile 0.88 arasında, ISSR için benzerlik katsayısını 0.40 ila 0.83 arasında vermiştir. RAPD ve ISSR’dan elde edilen dendogramlar karşılaştırıldığında 6 patotipin gruplar içinde gruplanması benzer değildir. RAPD ve ISSR 3D ölçeklendirmeleri UPGMA sonuçlarını desteklemiştir ve altı S. graminicola patotipi arasında ilişkiyi açıklamıştır.
Sclerospora graminicola downy mildew izolatlarının farklı kültüvarlar ve moleküler markörler kullanılarak karakterizasyonu
Fourteen differential cultivar sets were used to analyze 27 downy mildew isolates of pearl millet, Sclerospora graminicola from different agroclimatic regions of India. Among 27 isolates, six distinct pathotypes were determined, the isolates were found to infect a narrow range of differential cultivars used and the disease incidence ranged between 0-98 per cent. Comparisons of RAPD and ISSR marker analyses were carried out on six S. graminicola pathotypes. A total of 20 random and 19 ISSR primers generated 152 and 297 bands and revealed 73.6 and 82.15% polymorphism which were able to distinguish the six pathotypes. In RAPD, maximum genetic distance index obtained was 0.1312 between pathotype-2 and pathotype-3 and minimum distance of 0.0231 was between pathotype-1 and pathotype-4. In ISSR 0.1302 was maximum between pathotype- 4 and pathotype-6 and minimum distance was 0.0124 from pathotype-1 and pathotype-5. UPGMA dendrograms obtained from cluster analysis data gave similarity coefficient ranging from 0.27 to 0.88 and 0.40 to 0.83 in RAPD and ISSR, respectively. Clustering of six pathotypes within groups was not similar when RAPD and ISSR derived dendrograms were compared. RAPD and ISSR 3D scaling of ordination supported the UPGMA results and clarified relationships among six S. graminicola pathotypes.
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- ISSN: 1303-3646
- Yayın Aralığı: Yılda 2 Sayı
- Yayıncı: Haliç Üniv. Fen Edebiyat Fak.
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