Laboratory Rearing of Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae) Wild Colony on Different Artificial Diets

Laboratory Rearing of Cotton Bollworm, Helicoverpa armigera (Hübner) (Lepidoptera:Noctuidae) Wild Colony on Different Artificial Diets

Yeşil kurt, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) domates tarlalarında görülen önemli bir zararlıdır. Konukçu bitkiye bağlı kalmaksızın tüm yıl boyunca laboratuar koşullarında zararlının biyolojisi, davranışı, insektisite dayanıklılık ve kontrol yaklaşımlarının incelenmesi son derece önemlidir. Bunu yapabilmek için zararlı devamlı olarak yapay diyet üzerinde yetiştirilmelidir. Çalışmanın amacı, yeşil kurdun laboratuvarda yetiştirilmesi ve adaptasyonuna yönelik olarak bilinen yapay diyetleri test ederek en iyi larva diyetinin tespit edilmesidir. Zararlı ile bulaşık örnekler Çanakkale’den toplanarak laboratuara getirildi. Barbunya unu, soya fasulyesi, mısır unu, nohut unu, buğday ruşeymi, Brewer’s mayası, torula mayası, sükroz, vitaminler ve koruyucu gibi temel içerikler kullanılarak altı farklı yapay diyet hazırlandı. Larvalar aynı zamanda deneme boyunca domates meyvesi üzerinde kontrol olarak yetiştirildi. Burada, laboratuvarda altı farklı yapay diyetin larva dönemi sayısı, larva ve pupa gelişme süresi, pupa ağırlığı ve ergin açılma oranı gibi bazı biyolojik parametreleri belirlendi. Sonuç olarak, orijinali Grapholita molesta için geliştirilmiş Diyet I’de larvalar gelişme süresini 11.6 ± 1.5 günde tamamlayıp, pupa ağırlığı 331.8 mg olmuştur. Orijinal olarak Lepidopteran türleri için geliştirilmiş olan ve burada Diyet II olarak adlandırılan diyette ise, yeşil kurt larvaları larva gelişme süresini 16.6 ± 1.4 günde tamamlamıştır. Yeşil kurt larvaları, Diyet I ve Diyet II’de başarılı bir şekilde dört generasyon boyunca yetiştirilmiştir. Çalışma sonuçları, Diyet I ve Diyet II’nin yeşil kurdun doğal kolonisinin yetiştirilmesinde ve adaptasyonunda besin olarak uygun olduğunu göstermiştir.

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Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi
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