Evaluation of Nested PCR assay for the Detection Aspergillus Species
Evaluation of Nested PCR assay for the Detection Aspergillus Species
In this research, we investigated the application of nested PCR assay for the identification of Aspergillus species. We obtained amplified products from fungal genera other than Aspergillus, showing the presence of cross-reactions with fungal DNA
___
- 1- Latge, J.P., Calderone, R. 2002. Host-microbe interactions: fungi Invasive human fungal opportunistic infections, Curr Opin Microbiol, 5, 355-358.
- 2- Mellado, E., Guerra, D.G., Estrella, M.C., Tudela, J.L.R. 2001. Identification of Two Different 14-α Sterol Demethylase-Related Genes (cyp51A and cyp51B) in Aspergillus fu migatus and Other Aspergillus species, J. Clin. Microbiol., 39, 2431- 2438.
- 3- Latge, J.P. 1999. Aspergillus fumigat us and Aspergillosis, Clin. Microbiol. Rev., 12, 310-350.
- 4- Bougnoux, M.E., Dupont, C., Mateo, J., Saulnier, P., Faivre, V., Payen, D., Chanoine, M.H.N. 1999. Serum is More Suitable than Whole Blood for Diagnosis of Systemic Candidiasis by Nested PCR, J. Clin. Microbiol, 37, 925-930.
- 5- Hendolin, P.H., Paulin, L., Koukıla-Kahköla, P., Anttila, V., Malmberg, H., Richardson, M., Ylikoski, J. 2000. Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens,J. Clin. Microbiol, 38, 4186-4192.
- 6- Polanco, A., Mellado, E., Castilla, C., Tudela, J.L.R. 1999. Detection of Candida alb icans in Blood PCR in a Rabbit Animal Model of Disseminated Candidiasis, Diagn. Microbiol. Infec. Dis., 34, 177- 183.
- 7- Yamakami Y., Hashımoto A., Tokımatsu I., Nasu M.. 1996. PCR Detection of DNA Specific for Aspergillus Species in Serum of Patients with Invasive Aspergillosis, J. Clin. Microbiol, 34, 2464- 2468.
- 8- Yamada, Y., Makimura. K., Mirhendi, H., Ueda, K., Nishiyoma, Y., Yamaguchi, H., Osumi, M. 2002. Comparison of Different Methods for Extraction of Mitochondrial DNA from Human Pathogenic Yeasts, Jpn. J. Infect. Dis., 55, 122-125.
- 9- Chen, X., Ramaine, C.P., Tan, Q., Schlagnhaufer B., Gıraldo, M.D.O., Royse, D.J., Huff, D.R. 1999. PCRBased Genotyping of Epidemic and Preepidemic Trichoderma Isolates Associated with gren Mold of Agaricus bisporus ., Appl Environ Microbiol., 65. 2674-2678.
- 10- Olgun A, Topal A. DNA’ nın analizi. Temizkan G, Yılmazer S, ve ark. 1998. Moleküler Biyolojide Kullanılan Yöntemler, İstanbul Üniversitesi Biyoteknoloji ve Genetik Mühendisliği Araştırma ve Uygulama Merkezi (BİYOGEM), Yayın no:1.
- 11- Yamakami, Y., Hashimoto, A., Yamagata, E., Kamberi, P., Karashima, R., Nagai, H., Nasu, M. 1998. Evaluation of PCR for Detection of DNA Specific for Aspergillus Species in Sera of Patients with Various Forms of Pulmonary Aspergillosis,J. Clin. Microbiol., 36, 3619-3623.
- 12- Melchers W.J.G., Verweıj P.E., Hurk P., Belkum A., Pauw B.E., Hoogkamp-Korstanje J.A.A., Meis J.F.G.M. 1994. General primer- Mediated PCR for detection of Aspergillus Species,J. Clin. Microbiol, 32, 1710-1717.
- 13- Cruzado M., Blanco J.L., Duran C., Andrino M., Garcia M.E. 2004. Evaluation of two PCR methodologies fort he detection of Aspergillus DNA, Rev. Iberoam Micol ; 21: 209-212