Evaluation of Nested PCR assay for the Detection Aspergillus Species

Evaluation of Nested PCR assay for the Detection Aspergillus Species

In this research, we investigated the application of nested PCR assay for the identification of Aspergillus species. We obtained amplified products from fungal genera other than Aspergillus, showing the presence of cross-reactions with fungal DNA

___

  • 1- Latge, J.P., Calderone, R. 2002. Host-microbe interactions: fungi Invasive human fungal opportunistic infections, Curr Opin Microbiol, 5, 355-358.
  • 2- Mellado, E., Guerra, D.G., Estrella, M.C., Tudela, J.L.R. 2001. Identification of Two Different 14-α Sterol Demethylase-Related Genes (cyp51A and cyp51B) in Aspergillus fu migatus and Other Aspergillus species, J. Clin. Microbiol., 39, 2431- 2438.
  • 3- Latge, J.P. 1999. Aspergillus fumigat us and Aspergillosis, Clin. Microbiol. Rev., 12, 310-350.
  • 4- Bougnoux, M.E., Dupont, C., Mateo, J., Saulnier, P., Faivre, V., Payen, D., Chanoine, M.H.N. 1999. Serum is More Suitable than Whole Blood for Diagnosis of Systemic Candidiasis by Nested PCR, J. Clin. Microbiol, 37, 925-930.
  • 5- Hendolin, P.H., Paulin, L., Koukıla-Kahköla, P., Anttila, V., Malmberg, H., Richardson, M., Ylikoski, J. 2000. Panfungal PCR and Multiplex Liquid Hybridization for Detection of Fungi in Tissue Specimens,J. Clin. Microbiol, 38, 4186-4192.
  • 6- Polanco, A., Mellado, E., Castilla, C., Tudela, J.L.R. 1999. Detection of Candida alb icans in Blood PCR in a Rabbit Animal Model of Disseminated Candidiasis, Diagn. Microbiol. Infec. Dis., 34, 177- 183.
  • 7- Yamakami Y., Hashımoto A., Tokımatsu I., Nasu M.. 1996. PCR Detection of DNA Specific for Aspergillus Species in Serum of Patients with Invasive Aspergillosis, J. Clin. Microbiol, 34, 2464- 2468.
  • 8- Yamada, Y., Makimura. K., Mirhendi, H., Ueda, K., Nishiyoma, Y., Yamaguchi, H., Osumi, M. 2002. Comparison of Different Methods for Extraction of Mitochondrial DNA from Human Pathogenic Yeasts, Jpn. J. Infect. Dis., 55, 122-125.
  • 9- Chen, X., Ramaine, C.P., Tan, Q., Schlagnhaufer B., Gıraldo, M.D.O., Royse, D.J., Huff, D.R. 1999. PCRBased Genotyping of Epidemic and Preepidemic Trichoderma Isolates Associated with gren Mold of Agaricus bisporus ., Appl Environ Microbiol., 65. 2674-2678.
  • 10- Olgun A, Topal A. DNA’ nın analizi. Temizkan G, Yılmazer S, ve ark. 1998. Moleküler Biyolojide Kullanılan Yöntemler, İstanbul Üniversitesi Biyoteknoloji ve Genetik Mühendisliği Araştırma ve Uygulama Merkezi (BİYOGEM), Yayın no:1.
  • 11- Yamakami, Y., Hashimoto, A., Yamagata, E., Kamberi, P., Karashima, R., Nagai, H., Nasu, M. 1998. Evaluation of PCR for Detection of DNA Specific for Aspergillus Species in Sera of Patients with Various Forms of Pulmonary Aspergillosis,J. Clin. Microbiol., 36, 3619-3623.
  • 12- Melchers W.J.G., Verweıj P.E., Hurk P., Belkum A., Pauw B.E., Hoogkamp-Korstanje J.A.A., Meis J.F.G.M. 1994. General primer- Mediated PCR for detection of Aspergillus Species,J. Clin. Microbiol, 32, 1710-1717.
  • 13- Cruzado M., Blanco J.L., Duran C., Andrino M., Garcia M.E. 2004. Evaluation of two PCR methodologies fort he detection of Aspergillus DNA, Rev. Iberoam Micol ; 21: 209-212
Journal of Applied Biological Sciences-Cover
  • ISSN: 1307-1130
  • Başlangıç: 2007
  • Yayıncı: Nobel Bilim ve Araştırma Merkezi
Sayıdaki Diğer Makaleler

Association of HLA- B7, B8, B27 and B51 with Genetic Protection Against Hepatitis B Virus

Mohammad NAJAFIZADEH, Bahador SARKARI, Naser FARHADI

Effects of 900 MHz Electromagnetic Fields Emitted by Cellular Phone on Total Cholesterol and Triglyceride Levels of Plasma in Syrian Hamsters ( mesocricetus auratus )

Habib Aghdam SHAHRYAR, Alireza LOTFI

Karyomorphological Studies of Ten Taxa of Barbarea (Cruciferae) from Turkey

Esra MARTİN, Yavuz BAĞCI, Ahmet SAVRAN

Medicinal Plant Diversity of Western Mediterrenean Region in Turkey

Hüseyin FAKİR, Bilgin GÜLLER, Mehmet KORKMAZ

Molecular Genetic Analysis of Three Turkish Local Silkworm Breeds (Bursa Beyazı, Alaca and Hatay Sarısı) by RAPD-PCR Method

Demet EROĞLU, Şükran ÇAKIR ARICA

Using Path Coefficient Analysis to Determine the Relationship between Yield and Yield Components of Dry Bean (Phaseolus vulgaris L.)

Oral DUZDEMIR

Morphological and Anatomical Investigations on Turkish Endemic Hieracium lasiochaetum ( Bornm. & Zahn) Sell & West (Asteraceae)

Adnan AKÇİN, Tülay AYTAŞ AKÇİN

Pro-inflammatory and Anti-inflammatory Cytokines in Vitreous Fluid of Patients with Proliferative Diabetic Retinopathy

M. A. Abdel HAMİD, T. EL-SERGANİ, A. T. SHOHEİR, A. A. SHOUMEN

Antioxidant Activity and Total Phenolic Content of Some Medicinal Plants in Urticaceae Family

Shaida Fariza SULAIMAN, Amir Modorresi CHAHARDEHI, Darah IBRAHIM

Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

Musa YAVUZ