Molecular Genetic Analysis of Three Turkish Local Silkworm Breeds (Bursa Beyazı, Alaca and Hatay Sarısı) by RAPD-PCR Method

In this study PCR-based Randomly Amplified Polymorphic DNA (RAPD) method was used to determine the genetic variation in three local silkworm breeds (Bursa Beyazı, Alaca and Hatay Sarısı) of Turkey. These three breeds were analysed with 23 RAPD primers. 34 polymorphic loci were observed with 5 primers of them. Percentage of polymorphic loci were varied between 17.65 and 55.88. Nei’s genetic distance was 0.0822 between Hatay Sarısı-Alaca and 0.1106 between Bursa Beyazı -Alaca. The diversity within the populations (Hs) was found 0.1334 and the magnitude of differentiation among the populations (GST) was found 0.2968

PDF

___

[1.] Nagaraju J., 2002. Application of genetic principles for improving silk production. Current Science, 83: 409-414.
[2.] Nagaraju J., 2003. Recent advances in molecular genetics of the silk moth, Bombyx mori. A review. 1- 13.
[3.] Nagaraja GM and Nagaraju J., 1995. Genome fingerprinting of the silkworm, Bombyx mori, using random arbitrary primers. Electrophoresis, 16:1633- 1638.
[4.] Nagaraju JG and Singh L., 1997. Assessment of genetic diversity by DNA profiling and its significance in silkworm, Bombyx mori. Electrophoresis, 18:1676-1681.
[5.] Nagaraju J and Goldsmith MR., 2002. Silkworm genomics-progress and prospects. Current Science, 83: 415-425.
[6.] Nagaraju J, Reddy KD, Nagaraja GM., Sethuraman BN., 2001. Comparison of multilocus RFLPs and PCR-based marker systems for genetic analysis of the silkworm, Bombyx mori. Heredity, 86: 588-597.
[7.] Tinker NA, Fortin MG, Mather DE., 1993. Random amplified polymorphic DNA pedigree relationships in spring barley. Theor. Appl. Genet., 85: 976-984.
[8.] Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV., 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
[9.] Clark AG and Lanigan CMS., 1993. Prospects for estimating nucleotide divergence with RAPDs. Mol. Biol. Evol., 10:1096-1111.