Elif UZ, Mahinur S. AKKAYA, Figen YILDIRIM GÜRSOY, Erdogan E. HAKKI

Genetic Relationship of Wild Einkorn Based on Geographical Distribution in Anatolia and Thrace using AFLP Markers

Triticu m monococcum L. ssp boe oticum Boiss., is the wild progenitor of domesticated einkorn. High throughput AFLP genetic analysis showed that the domestication of einkorn started in the northern part of the Fertile Crescent, near the Karacadag Mountains, Southeastern Turkey [1]. This study assesses the genetic distribution and the diversity of wild einkorn throughout Turkey, using total of 59 accessions from 22 locations in four different geographical regions. In our study, the four selective combinations of AFLP markers (E+ACC/M+ACT, E+ACC/M+ATA, E+ACT/M+ATA, and E+ATC/M+AAG) resulted in 161 AFLP marker loci. Phylogenetic trees for individual accessions and populations based on geographical regions were obtained using 'PopGen-32' population genetic analysis software. East and Southeast samples were genetically closest to each other among the samples from other regions. The samples from West, Northwest, and Central Anatolia were clustered together

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[1] Heun M, Schäfer-Pregl R, Klawan D, Castagna R, Accerbi M, Borghi B, Salamini F (1997) Site of einkorn wheat domestication identified by DNA fingerprinting. Science 278:1312–1314
[2] Renfrew JM (1969) The Archeological evidence for the domestication of plants: methods and problems. The Domestication and Exploitation of Plants and Animals (Peter J. Uoko and G.W. Dimbley, eds). Duckworth, London.
[3] Ucko PJ, Dimbleby GW(eds.) (1969) The Domestication and Exploitation of Plants and Animals. Proc Meeting Institute of Archaeology, London University. Duckworth, London pp.581
[4] Harlan JR (1981) The early history of wheat: Earliest traces to the sack of Rome. in L.T. Evans and W. Peacock (eds.) Wheat Science Today or Tomorrow. Cambridge, U.K.: Cambridge University Press.
[5] Perrino P, Hammer K (1984) The Farro: further information on its cultivation in Italy, utilization, and conservation (1). Genet Agr 38:303-311
[6] Özkan H, Brandolini A, Schäfer-Pregl R, Salamini F (2002) AFLP Analysis of a Collection of Tetraploid Wheats Indicates the Origin of Emmer and Hard Wheat Domestication in Southeast Turkey. Mol Biol Evol 19:1797–1801
[7] Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabaeu M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res, 23:4407-4414
[8] Marsan A., Castiglioni P, Fusari F, Kuiper M, Motto M (1998) Genetic diversity and its relationship to hybrid performance in maize as revealed by RFLP and AFLP markers. Theor Appl Genet 96:219-227
[9] Altintas S, Toklu F, Kafkas S, Kilian B, Brandolini A, Ozkan H (2008) Estimating genetic diversity in durum and bread wheat cultivars from Turkey using AFLP and SAMPL markers. Plant Breeding 127:9-14
[10] Barrett BA, Kidwell KK, Fox PN (1998) Comparison of AFLP and pedigree-based genetic diversity assessment methods using wheat cultivars from the Pacific Northwest. Crop Sci, 38:1271-1278
[11] Becker J, Vos P, Kuiper M, Salamini F, Heun M (1995) Combined mapping of AFLP and RFLP markers in barley. Mol Gen Genet 249:65-73
[12] Eivazi AR, Naghavi MR, Hajheidari M, Pirseyedi SM, Ghaffari MR, Mohammadi SA, Majidi I, Salekdeh, GH, Mardi M (2008) Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Ann Appl Biol 152:81- 91
[13] Ellis RP, McNicol JW, Baird E, Booth A, Lawrence P, Thomas B, Powell W (1997) The use of AFLPs to examine genetic relatedness in barley. Mol Breed 3:359-369
[14] Hongtrakul V, Huestis G, Knapp SJ (1997) Amplified fragment length polymorphisms as a tool for DNA fingerprinting sunflower germplasm: genetic diversity among oilseed inbred lines. Theor Appl Genet 95:400-407
[15] Keim P, Schupp JM, Travis SE, Clayton K, Zhu T, Shi L, Ferreira A, Webb DM (1997) A highdensity soybean genetic map based on AFLP markers.” Crop Sci 37:537-543
[16] Maheswaran M, Subudhi PK, Nandi S, Xu JC, Parco A, Yang DC, Huang N (1997) Polymorphism, distribution, and segregation of AFLP markers in a doubled haploid rice population. Theor Appl Genet 94:39-45
[17] Qi X, Stam P, Lindhout P (1998) Use of locusspecific AFLP markers to construct a highdensity molecular map in barley. Theor Appl Genet 96:376-384
[18] Schut JW, Qi X, Stam P (1997) Association between relationship measures based on AFLP markers, pedigree data and morphological traits in barley. Theor Appl Genet 95:1161-1168
[19] Zabaeu M, Vos P (1993) Selective restriction fragment amplification: a general method for DNA fingerprinting. European Patent Application. publication no. EP 534858A1.
[20] Yildirim F, Akkaya MS (2006) DNA fingerprinting and genetic characterization of Anatolian Triticum sp. using AFLP markers. Genet Resour Crop Evol 53:1033-1042.
[21] Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgian J Botany 129:157.
[22] Nei M (1987) Molecular Evolutionary Genetics. Columbia University press, New York, NY USA.
[23] McDermott JM, McDonald BA (1993) Gene flow in plant pathosystems. Ann Rev Phytopathol, 31:353–373.
[24] Podani J (1994) Multivariate data analysis in ecology and systematics– a methodological guide to the SYN-TAX 5.0 package. Ecol Comp Ser 6: 1–316. SPB Academic Publishing.
[25] Podani J (1997) SYN-TAX 5.1-pc. Multivariate Data AnalysisPackage. Scientia, Budapest.
[26] Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals.” Genetics 89:583- 590.
[27] Crow JF (1986) Basic Concepts in Population. Quantitative, and Evolutionary Genetics. W.H. Freeman & Co, New York.