Musa YAVUZ

Bacterial Protein Correction on In situ Digested Samples with Purine or NDF Methods

In situ ruminal digestion procedure is known procedure for estimating ruminal digestion. In situ procedure requires correction for microbial contaminations, and the purine method has been used to correct microbial attachments. The neutral detergent fiber (NDF) method has been proposed as an alternative to the purine method. Objectives of this experiment were to compare purine and NDF method for microbial correction. Alfalfa hay, bermudagrass hay, tall fescue hay, corn and soybean meal samples were selected. Chemical analyses of dry matter (DM), crude protein (CP), NDF, Acid detergent fiber (ADF), NDF-protein (NDIP), ADF-protein (ADIP), lignin, crude oil and ash were determined on selected samples. In the experiment, samples were inserted into the rumen of a cannulated cow for 6, 12, 24, 72, and 96 h to determine in situ digestion. The average microbial purine to nitrogen ratio was determined to be 11.43. In situ digested tall fescue microbial protein corrected CP values with purine and NDF methods were similar within all hours, except 96 h. Other In situ digested sample purine procedure had some interference during sample reading and standard errors were not acceptable level. There were not enough data to compare both methods for other samples

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[1] ANKOM Technology. Daisy II 200/220 in vitro incubator operator’s manual. Fairport, NY. [2] AOAC. 1998. Official methods of analysis. Association of Official Analytical Chemists, Arlington, VA.
[3] Klopfenstein, T. J., R. A. Mass, K. W. Creighton, and H. H. Patterson 2001. Estimating forage protein degradation in the rumen. J. Anim. Sci. 79 (E. Suppl.):E208-E217.
[4] LECO Corporation. Nitrogen Analyzer. St. Joseph, MI.
[5] Lehninger, A. L., D. L. Nelson, and M. M. Cox. 1993. Principle of biochemistry (Second edition). Worth publishers, New York, NY.
[6] Madsen, J., and T. Hvelplund. 1994. Prediction of in situ degradability in the rumen. Results of a European ringtest. Livest. Prod. Sci. 39:201.
[7] Makkar, H.P.S., and B.A. Dehority. 1999. Purine quantification in digesta from ruminants by spectrophotometric and HPLC methods. Br. J. Nutr. 81:107.
[8] Mass, R. A., G. P. Lardy, R. J. Grant, and T. J. Klopfenstein. 1999. In situ neutral detergent insoluble nitrogen as a method for measuring forage protein degradability. J. Anim. Sci. 77:1565.
[9] Marshak, A., and H. J. Vogel. 1951. Microdetermination of purines and pyrimidines in biological materials. J. Biol. Chem. 189:597.
[10] NRC. 1996. Nutrient Requirements of Beef Cattle (7th Ed.). National Academy Press, Washington, D. C.
[11] Obispo, N.E., and B. A. Dehority. 1999. Feasibility of using total purines as a marker for ruminal bacteria. J. Anim. Sci. 77:3084.
[12] Qrskov, E. R. 1982. Protein Nutrition in Ruminants. Academic Press Inc., San Diego, CA.
[13] SAS Institute, Inc. 2001. SAS/STAT Technical Report P-229. SAS/STAT Software: Changes and Enhancements, Release 8.02. Cary, NC.
[14] Saxton, A. M. 2001. PDMIX800 and MMAOV SAS file macros. University of Tennessee, Knoxville.
[15] Ushida, K., B. Lassalas and J. P. Joany. 1985. Determination of assay parameters for RNA analysis in bacterial and duodenal samples by spectrophotometry. Influence of sample treatment and preservation. Reprod. Nutr. Dev. 25:1037.
[16] Vanzant, E. S., R. C. Cochran, and E. C. Titgemeyer. 1998. Standardization of in situ techniques for ruminant feedstuff evaluation. J. Anim. Sci. 76:2717.
[17] Zinn, R. A., and F. N. Owens. 1986. A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci. 66:157