Çiğdem DİKEL, Yasemen YANAR, Onur DEMİRKOL

İskenderun Körfezi’nden avlanan derin su pembe karidesi (Parapenaeus longirostris)’nin atıklarından elde edilen astaksantinin ekstraksiyonu ve karakterizasyonu

Bu çalışmanın amacı, düşük değerli bir hammadde olan karides atıklarından yüksek değerli bir pigment olan astaksantinin izolasyonu için basit ve etkili bir yöntem önermektir. Bu çalışmada ekstraksiyon çözücüsü olarak aseton kullanılmıştır. TLC (Thin Layer Chromatography) için mobil faz olarak 3/7(v/v) oranında aseton/ heksan kullanılmıştır. Çalışmada kullanılan örnekler Parapenaeus longirostris türüne ait olup İskenderun Körfezi/ Türkiye’de faaliyette bulunan yerel balıkçılardan elde edilmiştir. Karides atıkları, buzla dolu steril bir kapta laboratuvara taşınmıştır. Karideslerin kullanılan atıkları sefalotoraks, karın kabuğu ve kuyruk kısmıdır. Sefalotoraks’a yapışan etler temizlenmiş, atıklar su ile yıkanmış ve örnekler 50 °C’ de etüvde kurutulmuştur. Polietilen torbalarla paketlenmiş ve kullanılıncaya kadar -18 °C’de saklanmıştır. Bu araştırmada astaksantin, organik çözücüler (petrol eteri ve aseton) kullanılarak karides kabuk atıklarından ekstrakte edilmiştir. Astaksantin pigmentinin karakterizasyonu, üç bant olarak serbest Astaksantin(Rf=0,43), Astaksantin monoester (Rf=0,56) ve Astaksantin diester (Rf=0,81) olarak tespit edilen Lorenz Todd standart kromatogramında belirtildiği Retardasyon faktörünü (Rf) karşılaştıran TLC ile gerçekleştirilmiştir.

Anahtar Kelimeler:

Astaksantin, Parapenaeus longirostris, Aseton, TLC

Extraction and characterization of astaxantin from the waste of deep water pink shrimp (Parapenaeus longirostris) obtained from İskenderun Bay

The aim of this study is to purpose a simple and effective method for the isolation of high-value pigment astaxanthin from shrimp waste, a low-value raw material. In this study, acetone was used as an extraction solvent. Mobile phase for TLC was acetone:hexane in the ratio 3:7 (v/v). The samples used in the study belong to the Parapenaeus longirostris species and were obtained from local fishermen operating in the Iskenderun Bay / Turkey. Shrimp waste was transported to the laboratory in a sterile container filled with ice. The wastes used in shrimp are the cephalothorax, abdominal shell and tail portion. Adhering meat from the cephalothorax was removed and the waste was washed under water and samples were dried at 50 °C with drying oven. Packed in polyethylene bags and stored at -18 °C until use. In this study, astaxanthin was extracted from shrimp shell waste using organic solvents (petroleum ether and acetone). Characterization of Astaxanthin pigment was performed with TLC buy comparing the Retardation Factor (Rf) as indicated in the Lorenz Todd standard chromatogram, in which three bands of Astaxanthin (Rf=0.36), Astaxanthin monoester (Rf=0.60) and Astaxanthin diester (Rf=0.75) were detected.

Keywords:

Astaxanthin, Parapenaeus longirostris, Aceton, TLC,

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