Sakine Ülküm ÇİZMECİ, Dursun Ali DİNÇ, Mustafa Numan BUCAK, Muhammed ÇİFTÇİ, Ömer Faruk YEŞİLKAYA, Vahit AĞIR, Hasan DOĞAN

Düvelerde antimüllerian hormonun ovum pick-up (opu) ile toplanan oosit verimine etkisi

Bu çalışmada Ovum Pick-Up (OPU) tekniği ile oosit verimi ile donörlerin Anti-Müllerian Hormon (AMH) konsantrasyonları arasındaki ilişkinin belirlenmesi amaçlanmıştır. Çalışmada 12 ila 15 aylık 10 sağlıklı Holstein düve kullanıldı. AMH ölçümleri Bovine VIDAS® Anti-Mülleian Hormon kitleri (Biomeriux, Marcy l'Etoile, Fransa) ile Mini Vidas cihazı kullanılarak yapıldı. Siklusun rastgele bir gününde toplam 67 OPU uygulaması yapıldı. Toplanan oositler kalitelerine göre sınıflandırıldı ve oositlerin canlılık değerlendirmesi, kümülüs-oosit kompleksindeki (COC) kümülüs bütünlüğü ile hücre tabakası sayısına ve sitoplazmalarının homojenliğine göre yapıldı. OPU uygulamalarında hayvan başına ortalama oosit sayısının 4-8 arasında olduğu belirlendi. Toplanan oosit sayıları ile plazma AMH seviyeleri arasında önemsiz bir negatif korelasyon görüldü. Donör ve ovaryum rezervinin belirlemede kullanılabilen AMH'nin, OPU başına düşen embriyo sayısını da artırabileceği düşünüldü. AMH'nin biyoteknolojik uygulamalarda donör seçiminde kullanılırken bu parametre açısından da değerlendirilebileceği sonucuna varıldı.

Anahtar Kelimeler:

AMH, düve, oosit, ovum pick-up

The effect of Anti-Mullerian hormone on yield of oocytes recovered by ovum pick-up (opu) in heifers

The aim of this study was to determine the relationship between the oocyte yield by the ovum pick-up (OPU) technique with the Anti-mullerian Hormone (AMH) concentrations of the donors. Ten healthy Holstein heifers aged 12 to 15 months were included in the study. AMH measurements were performed with Bovine VIDAS® Anti-Mülleian Hormone kits (Biomeriux, Marcy l’Etoile, France) using the Mini Vidas device. A total of 67 OPU sessions were performed on a random day of the cycle. Oocytes were classified according to their quality, and viability evaluation of oocytes was made according to the cell layer number and cumulus integrity in the cumulus-oocyte complex (COC), the homogeneity of their cytoplasm. The average oocyte yield in OPU sessions per animal was range from 4–8. There was an significant negative correlation between the collected oocyte numbers and plasma AMH levels. In conclusion, it was observed that AMH concentration did not affect the number of viable and the quality oocytes collected in weekly OPU administration in animals. It was thought that OPU applications performed without knowing the day of the cycle did not provide the expected correlation with AMH data.

Keywords:

AMH, heifer, oocyte, ovum pick-up,

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