Semiha KANAÇ, Ercan KESKİN, Deniz ULUIŞIK

Serulein ile Akut Pankreatit Oluşturulan Ratlara Timokinon Uygulamasının Bazı Sitokinler Üzerine Etkileri

Çalışmada serulein ile deneysel akut pankreatit oluşturulan ratlara timokinon uygulamasının bazı sitokinler üzerine olası etkilerinin değerlendirilmesi amaçlandı. K grubundaki hayvanlara herhangi bir uygulama yapılmadı. TQ grubundaki hayvanlara 9 gün süre ile günde 20 mg/kg timokinon intraperitoneal olarak verildi. AP grubundaki hayvanlarda araştırmanın 7. gününde 50 µg/kg ve 2 saat sonra 25 µg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. AP+TQ grubundaki hayvanlara günde 20 mg/kg olmak üzere 9 gün süre ile intraperitoneal olarak timokinon uygulandı ve 7. günde timokinon uygulamasından 2 saat sonra 50 µg/kg ve bundan 2 saat sonra 25 µg/kg seruleinin intraperitoneal olarak uygulanmasıyla akut pankreatit oluşturuldu. Alınan kan örneklerinde Tümör Nekroz Faktör (TNF)-α, Interlökin (IL)-6, IL-8, IL-10, Aspartat Aminotransferaz (AST) ve Alanin Aminotransferaz (ALT) düzeyleri belirlendi. Çalışmada akut pankreatitli grubun TNF-α düzeyi kontrol grubuna göre anlamlı olarak yüksek bulunurken, timokinon uygulanan akut pankreatitli grupta TNF-α düzeyi akut pankreatitli gruba göre anlamlı olarak daha düşük bulundu. IL-6 ve IL-8 düzeyleri akut pankreatit grubunda kontrol grubuna göre yüksek bulundu. Timokinon uygulanan akut pankreatitli ratlarda IL-6 ve IL-8 düzeyleri akut pankreatitli gruba göre anlamlı oranda düşüktü. Akut pankreatitli grupta AST ve ALT düzeyleri kontrol grubuna göre önemli oranda yüksek bulunurken, akut pankreatitli ratlara timokinon uygulamasıyla her iki enzim düzeyinin akut pankreatitli gruba göre anlamlı olarak düşük olduğu belirlendi. Çalışmada timokinon ön uygulaması yapılan akut pankreatitli ratlarda elde edilen bulgular timokinonun pankreatite bağlı olarak gelişen inflamasyonu hafiflettiği şeklinde değerlendirilebilir.

Anahtar Kelimeler:

Rat, Serulein, Sitokin, Timokinon

Effects of thymoquinone on some cytokine levels in cerulein-induced acute pancreatitis

In this study, it was aimed to evaluate the possible effects of thymoquinone administration on some cytokine levels in rats with experimental acute pancreatitis. No application was made the animals in group K. TQ group animals were intraperitoneally given 20 mg/kg thymoquinone daily for 9 days. In the AP group animals, acute pancreatitis was induced by intraperitoneal administration of cerulein as first dose 50 µg/kg and 2 hours later 25 µg/kg second dose on the 7th day of the study. Animals in the AP+TQ group were intraperitoneally administered 20 mg/kg thymoquinone daily for 9 days. On the 7th day of the study, after 2 hours from thymoquinone administration, acute pancreatitis was induced by intraperitoneal administration of cerulean as 50 µg/kg and 2 hours later 25 µg/kg. TNF-α, IL-6, IL-8, IL-10, AST and ALT levels were determined in the blood samples taken from all animals. In the study, TNF-α level was found to be importantly higher in the acute pancreatitis group compared to the control group, while TNF-α level was significantly lower in the acute pancreatitis group treated with thymoquinone than the acute pancreatitis group. IL-6 and IL-8 levels were higher in the acute pancreatitis group compared to the control group. IL-6 and IL-8 levels were found to be significantly lower in rats with acute pancreatitis treated with thymoquinone compared to the group with acute pancreatitis. While AST and ALT levels in the acute pancreatitis group were significantly increased when compared with the control group, both enzyme levels in the acute pancreatitis group treated with thymoquinone administration were found to be significantly lower than the rats with acute pancreatitis. In the study, the findings obtained in rats with acute pancreatitis which were pre-treated with thymoquinone can be evaluated as that thymoquinone alleviates inflammation due to pancreatitis.

Keywords:

Cerulein, Cytokine, Rats, Thymoquinone,

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