Bekir YAŞAR, Bartu BADAK, Mehmet Burak ÖZTOP

İRİNOTECAN UYGULANAN RATLARDA DENEYSEL KARACİĞER REZEKSİYONU SONRASINDA GRANÜLOSİT KOLONİ STİMÜLE EDİCİ FAKTÖRÜN REJENERASYON ÜZERİNE ETKİLERİ

Giriş: Çeşitli nedenlerle gerçekleştirilen karaciğer rezeksiyonları sonrasında meydana gelen karaciğer rejenerasyonunun mekanizmalarının daha iyi anlaşılması gerekmektedir. Bu çalışmada granülosit koloni uyarıcı faktörün karaciğer rejenerasyonu üzerindeki etkisini değerlendirmeyi amaçladık. Gereç ve Yöntem: Elli adet Sprague-Dawley albino rat 5 gruba ayrıldı.Grup A: Sham grubu (n: 10), Grup B: Karaciğer rezeksiyon grubu (n: 10), Grup C: G-CSF + Karaciğer rezeksiyon grubu (n: 10), Grup D: İrinotekan + Karaciğer rezeksiyon grubu (n: 10), Grup E: İrinotekan + G-CSF + Karaciğer rezeksiyon grubu (n: 10). Karaciğer sadece grup A'daki sıçanlarda açığa çıkarıldı. Grup B'de tüm sıçanlara laparotomi ve ardından karaciğer rezeksiyonu yapıldı. Grup C'de laparotomi sonrası karaciğer rezeksiyonu yapıldı ve 1 milyon ünite tek doz G-CSF intraperitoneal olarak uygulandı. Grup D'de laparotomi sonrası karaciğer rezeksiyonu yapıldı ve sıçanlara ameliyattan 7 gün önce 300 mg / m2irinotekan uygulandı. Grup E'ye operasyondan 7 gün önce 300 mg / m2 irinotekan uygulandı; laparotomi sonrası karaciğer rezeksiyonu yapıldı ve aynı anda 1 milyon ünite G-CSF verildi. Çalışmada kan örneklerinde AST, ALT ve MDA değerlendirildi. Karaciğer rejenerasyonuna yönelik PCNA immünohistokimyasal olarak, mitotik indeks ise rutin H&E yöntemiyle belirlendi. Ayrıca dokuda MDA düzeyinin belirlenmesi için biyokimyasal inceleme yapıldı Bulgular: Kan AST ve ALT düzeyleri gruplar arasında anlamlı farklılık gösterirken doku/serum MDA düzeyleri gruplar arasında anlamlı farklılık göstermedi. Mitotik indeks ve PCNA indeksi oranları karşılaştırılmış ve gruplar arasında anlamlı fark bulunmuştur. Karaciğerdeki yağ ve fibroz oranları, gruplar arasında benzerdi. Karaciğer rejenerasyonu, grup E ile grup C arasında istatistiksel olarak benzer iken, grup E'de karaciğer fibrozu ve adipozite anlamlı olarak artmıştır Sonuç: Granülosit koloni uyarıcı faktör, karaciğer rejenerasyonunu artırdı ancak fibroz ve yağlanma üzerinde anlamlı bir etkiye sahip değildi.

THE EFFECTS OF GRANULOCYTE COLONY STIMULATING FACTOR ON REGENERATION AFTER EXPERIMENTAL LIVER RESECTION IN IRINOTECAN ADMINISTERED RATS

Introduction: The mechanisms of liver regeneration that occur after liver resections performed for various reasons should be better understood. In this study aim to evaluate the effect of granulocyte colony stimulating factor on liver regeneration. Material and Method: Fifty Sprague-Dawley albino rats were divided into 5 groups. Group A: Sham group (n: 10), Group B: Liver resection group (n: 10), Group C: G-CSF + Liver resection group (n: 10), Group D: Irinotecan + Liver resection group (n: 10), Group E: Irinotecan + G-CSF + Liver resection group (n: 10). The liver was only exposed in rats in group A. In group B, all rats underwent laparotomy followed by liver resection. In group C, liver resection was performed following laparotomy, and 1 million units of a single dose of G-CSF was administered intraperitoneally. In group D, liver resection was performed after laparotomy, and the rats were administered 300 mg/m2irinotecan 7 days preoperatively. In group E, 300 mg/m2irinotecan was administered 7 days before the operation; after the laparotomy, liver resection was performed, and 1 million units of G-CSF was given simultaneously. In the study, AST, ALT and MDA were evaluated in blood samples. PCNA for liver regeneration was determined immunohistochemically and mitotic index was determined by routine H&E method. In addition, biochemical examination was performed to determine the MDA level in the tissue. Results: Blood AST and ALT levels showed significant difference between the groups but tissue/serum MDA levels showed no significant difference between the groups. The ratio of mitotic index and PCNA index were compared, and a significant difference was found between the groups. Liver regeneration was statistically significant in group E compared to that of group D. Fatty and fibrosis rates in the liver were similar between the groups. Liver regeneration was statistically similar between group E and group C, whereas liver fibrosis and adiposity were significantly increased in group E. Conclusion: Granulocyte colony stimulating factor increased liver regeneration but had no significant effect on fibrosis and fattening.

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