İhsan KISADERE, Hakan TAVŞANLI, Mehmet Faruk AYDIN, Özkan DEMİRBAŞ

Kadmiyuma (CD) maruz kalan dişi sıçanlarda Lactobacillus plantarum (LP) uygulamasının bazı kan parametreleri ve ANAE-pozitif lenfosit oranları üzerine kısmi koruyucu etkileri

Bu çalışmada, kronik olarak kadmiyuma (CD) maruz kalan dişi sıçanlarda oral Lactobacillus plantarum (LP) uygulamasının bazı kan parametreleri ile ANAE-pozitif lenfosit oranları üzerindeki etkilerini araştırmayı amaçladık. Bu araştırmanın yapılabilmesi için ratlar kontrol (K, n = 8), kadmiyum (CD, n = 8), LP (n = 8) ve CDLP (n = 8) olmak üzere dört eşit gruba ayrıldı. CD (2.04 mg/mL) oral olarak CD ve CDLP gruplarına 28 gün uygulandı. Ayrıca LP ve CDLP gruplarına aynı anda aktif-canlı LP (yaklaşık 108 - 109 cfu/mL) oral olarak uygulandı. C grubu hayvanlara çalışma süresince sadece taze su ve standart rat yemi verildi. Uygulamaların ardından hayvanlar dekapite edilerek bazı kan parametreleri (WBC ve farklı alt tipleri, RBC, HCT, MCV, MCH, MCHC ve PLT) ve ayrıca ANAE-pozitif lenfosit oranlarının değerlendirilmesi için kan örnekleri alındı. WBC sayıları diğer deney gruplarına göre CD grubunda en yüksek olarak belirlendi (p < 0,05). Öte yandan, LP uygulaması CDLP grubu hayvanlarda WBC sayılarında CD grubuna göre önemli bir iyileşmeye neden oldu (p < 0,05). NEU sayıları en yüksek CD grubunda saptanmakla birlikte, CDLP grubunda oral LP uygulamasına bağlı olarak bu sayılarda düşüş gözlemlendi (p < 0,05). Çalışmada CD uygulamasına bağlı olarak artan PLT değerleri, CDLP grubunda CD grubuna oranla LP diyeti kullanılarak düzeltildi (p < 0,05). Sonuç olarak LP uygulaması, dişi sıçanların kan parametrelerini CD'nin zararlı etkilerinden kısmen korumuştur.

Anahtar Kelimeler:

Lactobacillus plantarum, kadmiyum, dişi rat, hematoloji, ANAE pozitif

PARTIAL PROTECTIVE EFFECTS OF L. PLANTARUM ADMINISTRATION ON SOME BLOOD PARAMETERS AND ANAE-POSITIVE LYMPHOCYTE RATIOS IN FEMALE RATS EXPOSED TO CADMIUM (Cd)

We aimed to research the influences of oral L.plantarum administration on some blood parameters and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in female rats exposed chronically to Cd. In order to perform this investigation, rats were separated into four equal groups as control (C, n = 8), cadmium (Cd, n = 8), L.plantarum (n = 8), and Cd+ L.plantarum (n = 8). Cd (2.04 mg / mL) was orally applicated to Cd and Cd+ L.plantarum groups for 28 days. In addition, active-live L.plantarum (approximately 108 - 109 cfu / mL) was applicated orally to L.plantarum and Cd+ L.plantarum groups at the same time. C group animals received only fresh water and standard rat food during the study. After the applications, animals were decapitated and blood samples were collected for evaluating of some blood parameters (WBC and its different sub-types, RBC, HCT, MCV, MCH, MCHC, and PLT) and also ANAE-positive lymphocyte ratios. WBC counts were determined the highest in the Cd group compared to other experimental groups (p < 0.05). On the other hand, LP administration caused a significant amelioration in WBC counts of Cd+ L.plantarum group animals when compared to Cd group (p < 0.05). Although NEU counts were detected the highest in the Cd group, these counts decreased due to oral L.plantarum administration in Cd+ L.plantarum group (p < 0.05). Increased PLT values due to CD administration were fixed by using LP diet in the Cd+ L.plantarum group compared to the Cd group in the study (p < 0.05). In conclusion, L.plantarum partially affects female rats’ blood parameters from the harmful effects of Cd.

Keywords:

Lactobacillus plantarum, kadmiyum, dişi rat, hematoloji, ANAE pozitif,

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