H.Hakan BOZKURT, Oktay ARDA, İ.Kamuran İLERİ, Suzan DAĞLIOĞLU, Serhat ALKAN

PBSE (Beltsville poultry semen extender) ve PBS (Phoshate buffered saline) sulandırıcılarının soğukta saklanan hindi spermatozoonlarının ultrastrüktürü üzerine etkileri

Bu araştırmada özel solüsyonlarla sulandırılarak soğukta saklanan hindi spermatozoonlarının ultrastrüktürel özellikleri incelenmiştir. 20 erkek hindiden masajla elde edilen spermalar pooling yöntemiyle tek kapta toplandı. Toplanan spermalar ikisi deneme biri kontrol olmak üzere üç gruba ayrıldı. Deneme gruplarından birincisi PBSE (Beltsville Poultry Semen Extender) ile ikincisi ise PBS (Fosfat Buffered Saline) ile 1:2 oranında sulandırıldı. Her iki deneme grubundaki spermalar, üç alt gruba ayrıldı. Bunlardan ilk grup örnekler ısısı +26°C'de iken bekletilmeden, ikinci gruptakilerin ısısı +5°C 'ye düşürülerek bekletilmeden, üçüncü gruptakiler ise +5°C de 24 saat bekletildikten sonra tesbit edilerek rutin elektron mikroskobik yöntemle incelendiler. Kontrol grubuna ait örnekler de doğrudan elektron mikroskobi için hazırlandılar. İncelememizde kontrol grubu örneklerde hindi spermatozoonunun tipik ultrastrüktürü izlendi. Spermaların sulandırılmasından hemen sonra tesbit edilen örneklerde spermatozoonlarda boyun veya orta-parçadan başlıyan bir bükülme izlendi. Bükülme oranı PBSE ile sulandırılan örnekte %23.8, PBS ile sulandırılan örnekte ise %22 olarak saptandı. Bu oranlar kontrol grubuyla karşılaştırıldığında istatistiksel olarak önemli değildi. +5°C'de tesbit edilen örneklerde bükülme gösteren spermatozoonların oranı kontrol grubu ile karşılaştırıldığında istatistiksel olarak önemli düzeyde artış gösterdi (PBSE için P

The effect of PBSE (Belstivlle poultry semen extender) and PBS (Phosphate duffered saline) semen extender on ultrasturucture of Turkley spermatozoa kept in cold

In this study, the effect of keeping diluted turkey semen in cold was searched at ultra structural level. Semen were collected from 20 male turkeys and pooled. Then the semen was divided into three parts by split-sample procedure. One part used for control. One part was extended (1:2) with PBS (Phosphate Buffered Saline) and the last one with PBSE (Beltsville Poultry Semen Extender). These diluted samples were divided further in there parts. The first part at dilution, the second part at the moment temperature reached +5°C, the third part at 24th hour at +5°C and the control group without any treatment were fixed for electron microscopy. Typical ultra structure of turkey spermatozoon were observed in the control group. Some spermatozoa had bending from neck or mid-piece region in the samples fixed at dilution. These bent spermatozoa were 22 % in the PBS sample and 23.8 % in the PBSE sample but these percentages were not statistically significant when they were compared with the control sample. In the samples at +5°C the bent spermatozoa were increased significantly (PBSE, P

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1. Bakst, M.R. (1980): Fertilizing capacity and morphology of fowl and turkey spermatozoa in hypotonic extender. J. Reproduction and Fertility 60:121-127.
2. Bakst, M.R.; Cecil, H.C.; Sexton, TJ (1991): Modification of Ethidium Bromide Exclusion Procedure for Evaluation of Turkey Semen. Poultry Science. 70:366-370.
3. Bakst, M.R. and Sexton, T.J (1979): Fertilizing capacity and ultrastructure of fowl and Turkey Spermatozoa Before and After Freezing. Biol. Reproduction. 12:632- 640.
4. Bedford, J•M. (1968):Ultrastructural Changes in the Sperm Head During Fertilization in the Rabbit. Am. I. Anat. 123, 329.
5. Bozkurt, H.H and Holley, M.C. (1995): Identification of a 53-kDa Antijen in the Fibrous Sheath of Avian Spermatozoa. Journal of Reproductive Immunology 29:149- 160
6. Bozkurt, H.H. and Woolley, D.M. (1992) : Ultrastructurel Features of Bull Spermatozoon Prepared by Rapid-Freezing and Deep-Etching. Proc. 12th Int. Animal Reproduction The Hague Netherlands, August 1992.
7. Bozkurt, H.H. and Woolley, D.M. (1993): Morphology of Nexin Links in Relation to Interdoublet Sliding in the Sperm Flagellum. Cell Motility and Cytoskeleton 24:109-118
8. El Zayat, S. and Van Tienhoven, A. (1961): Effect of choloride ions on cook spermatozoa. American J. Physiology. 200:819-823.
9. Evrim, M. and Giines H. (1994): Biyometri Ders NotIan. 1.U. Veteriner Fak. Yay. No:14
10. Harris, G.C.; Thurston R.J•; Cundall, J. (1973): Changes in the Ultrastructure of the Fowl Spermatozoon Due to Rapid Frezee-Thaw. Journal of Fert. 34:389-394.
11. Maeda, T.; Terata, T.; Tsutsumi, Y. (1986): Studies of Factors Causing Abnormal Acrosomes and Crooked-Necks in Fowl Spermatozoa During Freezing and Thawing. British Poultry Sci. 27: 695-702.
12. Sexton,T.J.(1984): Breeding By Artificial Insemination. Reproductive Biology of Poultry: 175-182
13. Sexton,T.J•(1988):Comparison of Commercial Diluents for Holding Turkey Semen 24 Hours at +5°C. Poultry Science. 67:131-134
14. Sexton, T.J. (1987): Effect of the Treatments and Age of Tom on Fretility of Unstroed Semen and Semen Held 18 Hours. Poultry Science. 66:1721-1726.
15. Sexton,T.J.; Giesen,A.F.(1982): Betsville Poultry semen Extender. 6. Holding Turkey Semen for Six Hours at +15 °C. Poultry Science. 61:1202-12082.
16. Thurston R.J. and Froman D.P. (1984): Determination of turkey sperm acrosin activitiy. Poultry Science. 63:194-195.
17. Xia, L.; Lain, M.F.; Ansah, G.A.; Buckland, R.B. (1986): Ultrastructure of Fresh and Frozen-Thawed Spermatozoa of High and Low Lines of Chickens. Poultry Science. 67:819-825.