Abit AKTAŞ, Suzan DAĞLIOĞLU, H.Hakan BOZKURT, M.Başak ULKAY

İnce bağırsak yüzey alanı hesaplanmasında iki histolojik metodun karşılaştırılması

Bu araştırmada ince bağırsağın villus yüzey alanı hesaplanmasında sterelojik dikey kesit alma yöntemi ile geometrik modelleme yöntemi karşılaştırıldı. Çalışmada 10 adet Broyler cinsi tavuğun ince bağırsağının Mercel divertikulumundan itibaren distal yönde alman 8 cm lik parçası kullanıldı. Geometrik model için, enine alınan bağırsak kesitleri ile enine ve boyuna çizgilerden oluşan (kare) grid kullanıldı. Dikey kesit için bağırsak parçaları boyuna açıldı. Enine ve boyuna çizgilerden oluşan grid zemin üzerine yerleştirilerek trimlendi ve kesitleri alındı. Kesitler sikloid sonda yardımı ile değerlendirildi. Çalışma sonucunda iki yöntem ile elde edilen bağırsak yüzey alanı sonuçları arasında istatistiksel olarak önemli fark olduğu tespit edildi.

Comparison of two histological methods for estimating surface area of small bowl

A geometric model and an unbiased estimation using vertical section for villous surface estimation were compared. Small bowels of 10 domestic fowls were dissected and 8cm of bowl segments from mercePs diverticulum towards distal direction were subjected to estimation. In geometric model transversal sections of bowel and a test lattice consist of vertical and horizontal test lines were used. For unbiased estimation; bowel was opened along its length, laid flat on a substratum and sectioned vertical to the substratum. Then a cycloid test lattice was used. It was found that the estimated total surface due to villi was significantly different between the two methods.

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