Dişi köpeklerde taze ve donmuş spermayla intravaginal yolla yapılan sun'i tohumlama uygulamalarında elde edilen fertilite oranları
Çalışmada kullanılan 32 adet dişi köpekten 16'sı taze sperma, kalan 16'sı ise %4 gliserollü Tris-früktoz-sitrik asit sulandırıcısında payet yöntemine göre dondurulmuş sperma ile intravaginal yolla tohumlandı. Optimal tohumlama zamanının tespitinde "Ovulasyon Test Kiti" kullanıldı ve yan kantitatif saptanan progesteron değerine bakılarak (1-8 ng/ml) taze ve donmuş sperma ile dişiler gün aşın 3 kez tohumlandı. Gebelik oranı taze sperma ile %81.25, dondurulmuş sperma ile %37.50 olarak saptandı ve aralarındaki fark önemli bulundu (p
Fertility rates of intravaginal artificial inseminations with fresh and frozen semen in the bitch
A total of 32 bitches were used in this study, out of these 16 bitches were inseminated with fresh semen and the other with semen frozen by paillette technique in 4% glycerol containing Tris-fructose-citric acid extender by intravaginal route. The optimal insemination time was determined by Ovulation Test Kit and inseminations were repeated in both groups every other day for 3 times considering the semi-quantitative progesterone value (1-8 ng/ml). Pregnancy rate was 81.25% for the fresh semen group and 37.50% for the frozen semen group. Pregnancy rates in the fresh semen group have been significantly higher than the frozen semen group (p
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