Broyler ve yumurtacı tavuk serumlarında, Salmonella enteritidis ve Salmonella typhimurium antikorlarının serolojik testlerle araştırılması ve deneysel infeksiyon çalışmaları

Bu çalışmada deneysel infeksiyon oluşturmak amacıyla civcivlere oral yolla Salmonella enteritidis ve Salmonella typhimurium verildi. 1x $10^{8}$ S. enteritidis verilen 10 adet 1 günlük civcivden 4 adedi 2, 5, 7 ve 13. günlerde öldü. Ölen hayvanların iç organlarından (karaciğer, dalak, sekal tonsiller ve barsak içeriği) etken izole ve identifiye edildi. 1x $10^{4}$ S. enteritidis ve S. typhimurium verilen 1 ve 8 günlük civcivlerde 8 hafta süreyle ölüm görülmedi. Ancak bu civcivlerden 2'şer hafta arayla alınan klokal svablardan etkenler aralıklı olarak izole ve identifiye edildi. Kesimhaneden elde edilen toplam 278 broyler serumunda ELISA ile %1.07 (3 serum) oranında pozitiflik saptandı. Aynı serumlarla yapılan çabuk lam aglütinasyon testinde hiç pozitiflik bulunmadı. Yumurtacı tavuklardan elde edilen 222 adet kan serumu örneğinde ise ELISA ile Salmonella antikorları yönünden toplam 38 serumda (%17.1) pozitiflik saptandı. Aynı serumlarla çabuk lanı aglütinasyon testinde ise 15 serumda (%6.7) pozitiflik bulundu.

Serological investigations on Salmonella enteritidis and Salmonella typhimurium antibodies in broiler and layer's and experimental infection studies

Summary: In this study, Salmonella enteritidis and Salmonella typhimurium were administered oral-ly to produce experimental infection in chicks. Ten, a day old chicks which were given 1x$10^{8}$ S. enteritidis that of 4 died on days 2, 5, 7 and 13 respectively. Animals died after the oral administration of S. enteritidis were autopsied and S. enteritidis was isolated from the liver, spleen caecal tonsils and intestinal content of the chicks. One and eight days old chicks which were given 1x $10^{4}$ S. enteritidis and S. typhimurium were observed for 8 weeks. None of the chicks died during this period. However, S. enteritidis and S. typhimurium were isolated from the cloacal swabs taken from the chicks 2 weeks intervals. Antibodies to Salmonella were detected in the sera of 1.07% of 278 broilers by ELISA but no antibody was by rapid slide agglutination test. Antibodies to Salmonella were detected in the sera of 17.1 % of 222 laying hens by ELISA. Antibodies were also detected in 6.7 % of same sera by RSA test.

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