Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content

The influence of low-intensity electromagnetic radiation (EMR) on the content of phenolic compounds (PC) in plants on different stages of ontogeny is discussed. A medicinal plant (Calendula officinalis L.) and agricultural crop Fagopyrum sagittatum G., diploid varieties and tetraploid varieties were chosen as objects of study. Microwave EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00 GHz (narrow range mode), has been selected to learn the physical effects on seeds, with a treatment exposure time of 20, 12, or 8 min. The experiments were conducted under laboratory and field conditions. Treated and control seeds were germinated by 100 pcs in a growth chamber on moistened filter paper at 22–24˚C in the dark and placed on the daylight from the third day. The level of PC in the seedlings was measured with the Folin-Ciocalteu reagent. It was found that in the EMR pretreated C. officinalis seedlings the level of PC is maximal on 14th day of germination. The wide mode treatment for 12 min results in a two-fold increase in the level of PC in juvenile shoots with respect to control. Different constitutional levels of phenolic compounds in the leaves of diploid and tetraploid varieties of F. sagittatum in the most critical phases of ontogeny were found. It is recommended to use EMR with a 12 min exposure time for C. officinalis. The narrow frequency mode with an exposure time of 20 min is preferable to use for most varieties of F. sagittatum.

Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content

The influence of low-intensity electromagnetic radiation (EMR) on the content of phenolic compounds (PC) in plants on different stages of ontogeny is discussed. A medicinal plant (Calendula officinalis L.) and agricultural crop Fagopyrum sagittatum G., diploid varieties and tetraploid varieties  were chosen as objects of study. Microwave EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00 GHz (narrow range mode), has been selected to learn the physical effects on seeds, with a treatment exposure time of 20, 12, or 8 min. The experiments were conducted under laboratory and field conditions. Treated and control seeds were germinated by 100 pcs in a growth chamber on moistened filter paper at 22–24˚C in the dark and placed on the daylight from the third day. The level of PC in the seedlings was measured with the Folin-Ciocalteu reagent. It was found that in the EMR pretreated C. officinalis seedlings the level of PC is maximal on 14th day of germination. The wide mode treatment for 12 min results in a two-fold increase in the level of PC in juvenile shoots with respect to control. Different constitutional levels of phenolic compounds in the leaves of diploid and tetraploid varieties of F. sagittatum in the most critical phases of ontogeny were found. It is recommended to use EMR with a 12 min exposure time for C. officinalis. The narrow frequency mode with an exposure time of 20 min is preferable to use for most varieties of F. sagittatum.

___

  • Zaprometov, M.N. (1993). Physiology and biochemistry of flavonoids. J. Physiology of plants, 40(6), 921–931. (in Russian)
  • Gorgun, Y., Mazets, Z., Shysh, S., Elovskaya, N. (2017). Influence of low-intensity electromagnetic radiation on the dynamics of accumulation of phenolic compounds in buckwheat plants tetraploid. «Modern Problems of Natural Science in Science and the Educational Process» Materials of the Republican Scientific and Practical Conference. Minsk (in Russian).
  • Jambazishvili, S.N. (1974). Presowing treatment of seeds of sugar beet by various electrophysical methods and their influence on productivity and quality of root crops: the dissertation author's abstract the candidate of agricultural sciences: 06.01.09, Tbilisi (in Russian)
  • Novikova, G.V. (1980). Substantiation and research of technological process of influence of EMF microwave on seeds of vegetable cultures: the dissertation of the candidate tehn. sciences: 05.20.01., Chelyabinsk (in Russian)
  • Tambiev, A.H., Gusev, M.V., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (1986). Stimulation of growth of cyanobacteria by millimeter electromagnetic radiation of low intensiveness. Trade Exibition Microbe-86. XIX Intern. Congr. Microbiol., September 7-13:Abstr. – Manchester, England, 1986. P. 380.
  • Deviatkov, N.D., Golant, M.B., Beckiy, O.V. (1991). Millimeter waves and their role in life processes, Moscow (in Russian)
  • Karpovich, V.A., Ermolovich, A.A., Mikhalenko, E.G., Barashkova, G.P., Novikov, O.T. (2004). New stimulating and disinfecting microwave technology for presowing processing of vegetable seeds. Agropanorama. 4, 17. (in Russian)
  • Klunduk, G.A. (2004). Substantiation of electrotechnological regimes of microwave treatment of flax seeds: the dissertation of the candidate tehn. sciences: 05.20.02. Krasnoyarsk, 156. (in Russian)
  • Tambiev, A.H., Kirikova, N.N., Beckiy, O.V., Gulaev, U.V. (2003). Millimeter waves and photosynthesizing organisms. J. Radio engineering, 175. (in Russian)
  • Tambiev, A.H. (2009). Regularities manifested by the action of low-intensity waves on photosynthetic organisms. J. Millimeter waves in biology and medicine, 56(4), 56-68. (in Russian)
  • Chilochi, A.A., Tyurina, Zh. P., Klapko, S.F., Stratan, M.V., Lablyuk, S.V., Dvornina, E.G., Kondruk, V.F. (2011). Effect of millimeter-range electromagnetic radiation on the biosynthesis of extracellular hydrolytic enzymes in Aspergillus and Penicillium micromycetes J. Surface Engineering and App. Electrochem, 47(6), 558–564.
  • Debouzya, J.-C., Crouziera, D., Dabouisa, V., Malabiaub, R., Bacheleta, C., Perrina, A. (2007) Effets biologiques des rayonnements millimétriques (94 GHz). Quelles conséquences à long terme? Biologic effects of millimeteric waves (94 GHz). Are there long term consequences? J. Pathologie biologie, 55(5), 246–255.
  • Volynets, A.P. (2013). Phenolic compounds in the life of plants. Minsk (in Russian)
  • Karpovich, B.A., Rodionova, V.N. (2003). The method of presowing treatment of seeds of vegetable or grain crops. Patent of Belarus, №5580. (in Russian)
  • State Pharmacopoeia of the Republic of Belarus (2008) V.2, p.381. (in Russian)
  • Wang, M., Simon, J., Aviles, I., He, K., Zheng, Q., Tadmor, Y. (2003). Analysis of antioxidative phenolic compounds in artichoke (Cynara scolymus L.) J. of agricultural and food chemistry, 51(3), 601-608.
  • Fuksman, I.L. (1999) Influence of natural and anthropogenic factors on the metabolism of substances of secondary origin in woody plants: the dissertation author's abstract of the doctor of biol. sciences: 03.00.16, St. Petersburg. (in Russian)
  • Zaprometov, M.N. (1974) Basics of biochemistry of phenolic compounds. Moscow. (in Russian)
  • Lomboeva, S.S., Tankhaeva, L.M., Olennikov, D.N. (2008) Dynamics of accumulation of flavonoids in the aerial part of the ortilia with a one-sided (Orthilia secunda (L.) House) J. Chemistry of plant raw material, 3, 86-88. (in Russian)
  • Kopach, O.V., Pushkina, N.V., Kuzovkova, A.A., Karpovich, V.A. (2015). Exposure of ultra high frequency low power electromagnetic field on the biologically active substances biosynthesis in Silybum marianum L. cell cultures. J. Proceedings of the National Academy of Sciences of Belarus, Biological Series, 2, 5-8. (in Russian)
  • Kovzunova, O.V., Pushkina, N.V., Аzizbekian, S.G. (2017) In vitro culture Silybum marianum (L.) as a potential source of target BAS. J. Problems of biological, medical and pharmaceutical chemistry, 20(7), 3-6. (in Russian)
  • Shysh, S., Mazets, Z., Shutava, H. (2017). Efficiency of water and water-alcohol extraction of Сalendula officinalis raw by using various kinds of presowing processing. J. Problems of biological, medical and pharmaceutical chemistry, 6, 35- 40. (in Russian)
International Journal of Secondary Metabolite-Cover
  • Başlangıç: 2014
  • Yayıncı: İzzet KARA
Sayıdaki Diğer Makaleler

Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Tunhan DEMİRCİ, Özlem ARAS AŞCI, Nilgün GÖKTÜRK BAYDAR

Molecular identification and phytochemical profiling of kamiling (wild toxic plant) using thin layer chromatography

Dana Theresa C. DE LEON, John Dave C. AQUİNO, Mary Jhane G. VALENTİNO, Jerwin R. UNDAN

Antioxidative Defence Mechanisms in Tomato (Lycopersicum esculentum L.) Plants Sprayed with Different Pesticides and Boron compounds

Köksal KÜÇÜKAKYÜZ, Mahmut YILDIZTEKİN, Hasan Sungur CİVELEK, Said NADEEM, Atilla Levent TUNA

Biological Activities of Wild Asparagus (Asparagus acutifolius L.)

Arzu KASKA, Ramazan MAMMADOV, Nahide DENİZ

Effect of Pre-/ Postnatal Hypoxia on Pyruvate Kinase in Rat Brain

Afag M. RASHİDOVA

Investigation of Chemical Composition, Antioxidant, Anticholinesterase and Anti-urease activities of Euphorbia helioscopia

Mehmet Emin DURU, Ebru DEVECİ, Gülsen TEL ÇAYAN

Plant Taxa Used in the Treatment of Diabetes in Van Province, Turkey

Abdullah DALAR

Analysis of Phytochemical Composition and Biological Activities of Verbascum cheiranthifolium var. cheiranthifolium stem and flowers

Abdullah DALAR, Oruc ALLAHVERDİYEV, Aydin Sukru BENGU

Electromagnetic radiation of low intensity as a factor of change of phenolic compounds content

Svetlana SHYSH, Zhanna MAZETS, Hanna SHUTAVA, Olga SYSHA, Yulia GORGUN

Cold Resistance of Plant Species of the Genus Vitex l. Introduced in M.M. GRYSHKO National Botanic Garden of NAS of Ukraine

Nataliia LEVCHYK, Volodymyr LEVON