Tunhan DEMİRCİ, Özlem ARAS AŞCI, Nilgün GÖKTÜRK BAYDAR

Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Madder (Rubia tinctorum L.) is a valuable plant rich in anthraquinones having dying properties and biological activities. This study was carried out to determine the effect of sodium chloride (NaCl) applications on the root growth and secondary metabolite accumulation in adventitious roots of madder. For this aim, adventitious roots derived from stem explants in in vitro conditions were cultured in MS medium containing different concentrations of NaCl (0, 1, 2, 3 and 4 g/l) for 7 days. Then roots were evaluated in terms of root growth index, total AQ, alizarin, purpurin and total phenolic contents. Based on the results, root growth decreased in line with the elevating level of NaCl while secondary metabolite accumulation significantly increased with NaCl applications compared to the controls. It was determined that NaCl at 3 g/l concentration was the most effective application in terms of total AQ, alizarin, purpurin and phenolic accumulation.

Anahtar Kelimeler:

Rubia tinctorum, root culture, in vitro, sodium chloride, secondary metabolite

Effects of NaCl applications on root growth and secondary metabolite production in madder (Rubia tinctorum L.) root cultures

Keywords:

Rubia tinctorum, root culture, in vitro, sodium chloride, secondary metabolite,

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Karuppusamy S. (2009) A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants Research, 3(13), 1222-1239.
Hussain M.S., Fareed S., Saba Ansari M., Rahman A., Ahmad I.Z., Saeed M. (2012) Current approaches toward production of secondary plant metabolites. Journal of Pharmacy & Bioallied Sciences, 4(1), 10-20.
Rao S.R., Ravishankar G.A. (2002) Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances, 20(2), 101-153.
Baydar H., Karadoğan T. (2006) Agronomic potential and industrial value of madder (Rubia tinctorum L.) as a dye crop. Turkish Journal of Agriculture and Forestry, 30(4), 287-293.
Kinnosuko O., Takahito I., Keiko K. (1991) Madders dye, In: Atsuchi, K. (Ed.), Plant Cell Culture in Japan, CMC. Japan, p. 138-142.
Kawasaki Y., Yukihiro G.O.D.A., Yoshihira K. (1992) The mutagenic constituents of Rubia tinctorum. Chemical and Pharmaceutical Bulletin, 40(6), 1504-1509.
Kalyoncu F., Çetin B., Sağlam H. (2006) Antimicrobial activity of common madder (Rubia tinctorum L.). Phytotheraphy Research, 20, 490-492.
Siva R., Palackan M.G., Maimoon L., Geetha T., Bhakta D., Balamurugan P., Rajanarayanan S. (2011) Evaluation of antibacterial, antifungal, and antioxidant properties of some food dyes. Food Science and Biotechnology, 20(1), 7-13.
Lajkó E., Bányai P., Zámbó Z., Kursinszki L., Szőke É., Kőhidai L. (2015) Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1). Cancer Cell International, 15(1), 119.
Orban N., Boldizar I., Szücs Z., Bela D. (2008) Influence of different elicitors on the synthesis of antraquinone derivatives in Rubia tinctorum L. cell suspension cultures. Dyes and Pigments, 77, 249-257.
Hussein E.A., Aqlan E.M. (2011) Effect of mannitol and sodium chloride on some total secondary metabolites of fenugreek calli cultured in vitro. Plant Tissue Culture and Biotechnology, 21(1), 35-43.
Nazif W., Perveen S., Saleem I. (2006) Status of micronutrients in soils of district Bhimber (Azad Jammu and Kashmir). Journal of Agricultural and Biological Science, 1(2), 35-40.
Fatima S., Mujib A., Tonk D. (2015) NaCl amendment improves vinblastine and vincristine synthesis in Catharanthusroseus: a case of stress signalling as evidenced by antioxidant enzymes activities. Plant Cell, Tissue and Organ Culture (PCTOC), 121(2), 445-458.
Ni J., Yang X., Zhu J., Liu Z., Ni Y., Wu H., Zhang H., Liu T. (2015) Salinity-induced metabolic profile changes in Nitraria tangutorum Bobr. suspension cells. Plant Cell, Tissue and Organ Culture (PCTOC), 122(1), 239-248.
Nartop P., Akay Ş., Gürel A. (2017) Rubia tinctorum L. hücre kültürlerinde tuz stresi ve inokülasyon oranlarının etkileri, Sakarya Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 21(3), 1-1.
Oksana S., Marian B., Mahendra R., Bo S.H. (2012) Plant phenolic compounds for food, pharmaceutical and cosmetiсs production. Journal of Medicinal Plants Research, 6(13), 2526-2539.
Kubota H., Sato K., Yamada T., Maitani T. (1995) Phytochelatins (class lll metallothioneins) and their desglycyl peptides induced by cadmium in normal root cultures of Rubia tinctorum. Plant Sciene, 106(2), 157-166.
Murashige T., Skoog F. (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473-497.
Schulte U., El-Shagi H., Zenk M.N. (1984) Optimization of Rubiaceae species in cell culture for the production of anthraquinones. Plant Cell Reports, 3, 51-54.
Shin S.H. (1989) Studies on the production of anthraquinone derivatives by tissue culture of Rubia species. Archives Pharmacal Research, 12, 99-102.
Singleton V.L., Rossi J.R. (1965) Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid. American Journal of Enology and Viticulture, 16, 144-158.
Nazif N.M., Rady M.R., El-Nasr M.S. (2000) Stimulation of anthraquinone production in suspension cultures of Cassia acutifolia by salt stress. Fitoterapia, 71(1), 34-40.
Jeong G.T., Park D.H. (2006) Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors. Applied Biochemistry and Biotechnology, 129-132.
Munns R. (2002) Comparative physiology of salt and water stress. Plant, Cell & Environment, 25(2), 239-250.
Akula R., Ravishankar G.A. (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior, 6(11), 1720-1731.
Lindsey K., Yeoman M.M. (1983) The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. Journal of Experimental Botany, 34(8), 1055-1065.
Ali R.M., Abbas H.M. (2003) Response of salt stressed barley seedlings to phenylurea. Plant Soil and Environment, 49(4), 158-162.
Jaleel C.A., Sankar B., Sridharan R., Panneerselvam R. (2008) Soil salinity alters growth, chlorophyll content, and secondary metabolite accumulation in Catharanthus roseus. Turkish Journal of Biology, 32(2), 79-83.
Mahajan S., Tuteja N. (2005) Cold, salinity and drought stresses: an overview. Archives of Biochemistry and Biophysics, 444(2), 139-158.
Naik P.M., Al–Khayri J.M. (2016) Abiotic and biotic elicitors–role in secondary metabolites production through in vitro culture of medicinal plants: A review, Journal of Advanced Research in Biotechnology, 1(2), 1-7.
Taiz L., Zeiger E. (2006) Plant physiology, 4th edn. Sinauer Associates Inc. Publishers, Massachusetts.
Selmar D. (2008) Potential of salt and drought stress to increase pharmaceutical significant secondary compounds in plants. Landbauforschung Volkenrode Agriculture and Forestry Research, 58, 139-144.