Bazı Ophrys Taksonlarında Labellum Mikromorfolojisi

Çalışmada 10 değişik Ophrys taksonuna ait örneklerde labellum mikromorfolojisi Taramalı Elektron mikroskobu (SEM) yöntemi ile incelenmiş ve sonuçlar karşılaştırmalı tablolar halinde sunulmuştur. Örnekler, araziden toplandığı sırada %70 alkole alınmış, bir kısmı da herbaryum kurallarına göre kurutularak teşhisleri yapılmıştır. Alkolden çıkarılan labellum örnekleri, bisturi ve iğnelerle stereomikroskop altında dissekte edilerek doğrudan _üzeri çift taraflı yapışkan bantlar bulunan_ ve SEM cihazına ait çelik aparatlara yerleştirilmiştir. Bir gece kadar bekletilerek alkolü tamamıyla uçurulan bu örnekler, doğrudan Taramalı Elektron Mikroskobu (SEM) ile incelenmiş ve labellum mikrofotoğrafları çekilmiştir. Bulgularımıza göre spekulum yapılarının birbirinden çok farklı olduğu, bazal, medyan, lateral bölgelerde tüy / papilla şekli ve dağılımının türden türe farklılık gösterdiği tespit edilmiştir. Ayrıca apeks bölgesinin O. fusca, O. lutea var. minor, O. speculum, O. mammosa, O. pseudomammosa, O. heldreichii, O. umbilicata, O. holoserica türlerinde de farklılık gösterdiği tespit edilmiştir. Özetle; 1. derecede labellum spekulum ve apeks yapısının, 2. derecede de bazal, medyan ve lateral bölge yüzey karakterlerinin türler arasında çok farklılık gösterdiği sonucuna varılmıştır.

Anahtar Kelimeler:

Orchidaceae, Ophrys, çiçek, labellum, morfoloji, petal

Labellum Micromorphology in Some Ophrys Taxa

In the study, the micromorphology of labellum in samples belonging to 10 different Ophrys taxa was examined by Scanning Electron Microscopy (SEM) method and the results were presented in comparative tables. While the samples were collected from the field, they were taken to 70% alcohol, some of them were dried according to the herbarium rules and identified. The labellum samples extracted from alcohol were dissected under the stereomicroscope with scalpel and needles and placed directly into the iron apparatus of the SEM device with double-sided adhesive tapes. These samples, whose alcohol was completely evaporated by waiting for one night, were examined directly with Scanning Electron Microscope (SEM) and labellum microphotographs were taken. According to our findings, it was determined that the speculum structures are very different from each other, and the shape and distribution of the hair / papilla in the basal, median and lateral regions differ from species to species.It was also determined that the apex region differs in O. fusca, O. lutea, O. speculum, O. mammosa, O. pseudomammosa, O. heldreichii, O. umbilicata, and O. holoserica species. In summary; It was concluded that the labellum speculum and apex structure of the 1st degree and the basal, median and lateral region surface characteristics of the 2nd degree differ widely between the species.

Keywords:

Orchidaceae, Ophrys, flower, labellum, morphology, petal,

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