The effects of high temperature on pollen grain characteristics in tomato (Lycopersicon esculentum M.)

Ondört domates genotipinde yüksek sıcaklığın çiçek tozu gelişimine etkileri çalışılmıştır. Dört Şanlıurfa yerli domates genotipi (U-4-10, U-64-16, U-2-29, U-117-2) Asya Sebze Araştırma ve Geliştirme Merkezi (AVRDC)’nden sağlanan sıcağa tolerant on genotiple karşılaştırmalı olarak incelenmiştir. Denemeler, optimum (OT, 28/21°C gündüz/gece), orta yüksek sıcaklık (MHT, 32/22°C gündüz/gece) ve yüksek sıcaklık (HT, 37/27°C gündüz/gece) olarak düzenlenen üç sıcaklık rejiminde yürütülmüştür. Üç sıcaklık rejiminde üretilen çiçek tozu sayısı (NPP), salınan çiçek tozu sayısı (NRP), çiçek tozu canlılık oranı (PV) ve in vitro çiçek tozu çimlenme oranı (PG) belirlenmiştir. Yüksek sıcaklık bütün genotiplerde üretilen çiçek tozu sayısı, salınan çiçek tozu sayısı, çiçek tozu canlılık oranı ve çiçek tozu çimlenme oranı önemli derecede azaltmıştır. Polen salınım ve çimlenme oranlarının yüksek sıcaklıkta meyve tutumu için ıslah programlarında kullanılabilecek en önemli faktörler olabileceği belirlenmiştir.

Domateste (Lycopersicon esculentum M.) yüksek sıcaklığın çiçek tozu özelliklerine etkileri

The effects of high temperature stress on pollen development were studied in fourteen tomato genotypes. Four Sanliurfa local genotypes (U-4-10, U-64-16, U-2-29, U-117-2) were compared with 10 heat tolerant tomato varieties received from Asian Vegetable Research and Development Center (AVRDC). The experiments were conducted under three temperature regimes, designated as optimum temperature (OT, 28/21°C day/night), moderate high temperature (MHT, 32/22°C day/night) and high temperature (HT, 37/27°C day/night). Number of pollen grain produced (NPP), number of pollen grain released (NRP), percentage of released pollen viability (PV) and in vitro released pollen germination rate (PG) were scored at three temperature regimes. HT significantly decreased the NPP, the NRP, the PV and PG for all genotypes. Pollen release and germination ability under high temperature were revealed to be the most important factors determining the fruit set ability and could be used in breeding programs aiming for better fruit set under high temperatures.

___

  • Abdelmageed, A.H, Gruda, N., Geyer B. 2003. Effect of high temperature and heat shock on tomato (Lycopersicon esculentum M.) genotypes under controlled conditions. Conference on International Agricultural Research for Development. Deutscher Tropentag, Göttingen, October 8-10.
  • Bohnert H.J., Nelson, D.E., Jensenay R.G., 1995. Adaptations to Environmental Stresses. The Plant Cell. 7: 1099- 1111.
  • Camejo D., Rodriguez, P., Morales, A.M., Amico, J.M., Torrecillas, A., Alarcon, J.J., 2005. High temperature effects on photosynthetic activity of two tomato cultivars with different heat susceptibility. Journal of Plant Physiology .162: 281-289.
  • Eti, S., 1991., Bazı meyve tür ve çeşitlerinde değişik in Vitro testler yardımıyla çiçek tozu canlılık ve çimlenme yeteneklerinin belirlenmesi. Cukurova Universitesi, Ziraat Fakultesi Dergisi: 69:80-91.
  • Firon, N., Shaked, R., Peet, M.M., Phari, D.M., Zamskı, E., Rosenfeld, K., Althan, L., Pressman, N.E. 2006. Pollen Grains of Heat Tolerant Tomato Cultivars Retain Higher Carbohydrate Concentration Under Heat Stress Conditions. Scientia Horticulturae: 109:212-217.
  • Houghton, J.T., Y. Ding, D. J. Griggs, M. Noguer, P. J. Van der Linden, X. Dai, K. Maskell, and C. A. Johnson, 2001. Climate Change 2001: The Scientific Basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change. Cambridge University Press, New York, p. 94.
  • Kinet, J.M., Peet, M.M., 1997. Tomato. In: Wien, H.C. (Ed.), The Physiology of Vegetable Crops. CAB International, Wallingford, UK, pp. 207–258.
  • Peet M.M., Sato S. Gardner, R.G. 1988. Comparing heat stress on male-fertile and male-sterile tomatoes to chronic, sub-acute high temperature stress. Journal of Experimental Botany. 21(2): 225-231.
  • Peet, M.M., Willits, D.H. Gardner, R.G., 1997. Responses of ovule development and postpollen production processes in male-sterile tomatoes to chronic, sub-acute high temperature stress. Journal of Experimental Botany. 48: 101-111.
  • Pressman, E., Peet, M.M., Phar, D.M., 2002. The Effect of heat stress on tomato pollen characteristics is associated with changes in carbohydrate concentration in developing anters. Annals of Botany . 90:631-636.
  • Sato, S., Kamiyama M., Iwata, T., Makita, N., Furukawa, H., Ikeda, H. 2006. Moderate increase of mean daily temperature adversely affects fruit set of Lycopersicon esculentum by disrupting specific physiological process in male reproductive development. Annals of Botany. 10:1093- 1098.
  • Sato, S., Peet, M.M., Gardner, R.G. 2001. Formation of partenocarpic fruit, undeveloped flowers and aborted flowers in tomato under moderately elevated temperatures. Scientia Horticulture. 90:243-254.
  • Sato, S., Peet, M.M., Thomas, J.F. 2000. Physiological factors limit fruit set of tomato (lycopersicon esculentum mill.) under chronic mild heat stress. Plant, Cell and Environment. 23:719-726.
  • Sato, S., Peet, M.M., Thomas, J.F. 2002. Determining critical pre-and post- anthesis periods and physiological processes in Lycopersicon esculentum Mill. exposed to moderately elevated temperatures. Journal of Experimental Botany. 53:1187-1195.
  • Thomas, J. M.G. Prasad, P. V.V. 2003. Plants and the Environment /Global Warming Effects. University of Florida, Gainesville, FL, USA.
  • Wahid A., Gelani, S., Ashraf, M., Foolad M.R. 2007. Heat tolerance in plants: An overview. Environmental and Experimental Botany. 61: 199-223.
  • Warner, R. M., Erwin, J. E., 2005. Naturally occurring variation in high temperature induced floral bud abortion across Arabidopsis thaliana accessions Plant, Cell and Environment. 28:1255–1266
Harran Üniversitesi Ziraat Fakültesi Dergisi-Cover
  • ISSN: 1300-6819
  • Yayın Aralığı: Yılda 4 Sayı
  • Yayıncı: İbrahim TOBİ