Murat DİKİLİTAŞ, Sadettin GÜRSÖZ, Bekir E. AK, Mustafa ÖZDEN

110 Ranacı üzerine aşılı şiraz üzüm ( vitis vinifera l.) çeşidinin nacl ve prolin uygulamalarına karşı fizyolojik ve biyokimyasal tepkileri

Bitkilerde moleküler düzeyde stres tolerans mekanizmasının tam olarak anlaşılamaması, ıslahprogramlarının stres koşullarına toleranslı bitki çeşitleri geliştirmedeki başarı düzeyinisınırlama ktadır. Islah programlarına alternatif olarak, stres koşulları altında yetişitirilen bitkilerintolerans seviyelerini artırmak için prolin gibi farklı organik bile şiklerin kullanımı artmaktadır. Buçalışmada, NaCl stresi altında yetiştirilen Şiraz üzüm çeşidinin NaC l ve prolin uygulamalarınafizyolojik ve biyokimyasal tepkileri araştırılmıştır . İyon akışı (EL), sürgün büyüme oranı (SBO),klorofil, prolin, malondialdehit (MDA), ve süperokside dismütaz (SOD), peroksidaz (POD),katalaz (CAT), polifenol oksidaz (PPO) gibi antioksidan enzim aktiviteler i ölçülmüştür. NaCl stre sseviyesine bağlı olarak, yaprak hücrelerindeki iyon akışı, klorofil degridasyonu, prolin ve MDAmiktarların da bir artış ölçülürken, prolin uygulanan gruplardan alınan yaprak örneklerinde ise buparametrelerde azalmalar belirlenmiştir. Araştırmada kullanılan prolin konsan trasyonları farklıseviyedeki NaCl stres koşullarında yetiştirilen Şiraz üzüm çeşidinin antioksidan enzim systemiüzerinde kısmi ola rak etkili olduğu gözlemlenmiştir . Araştırmanın sonuçları, prolinin, bitki hücrezarı faz değişimi, lipid peroksidasyonu ve antioksidan enzim sistemlerinde aktif rol almışolababileceğini işaret etmektedir.

Physiological and biochemical responses of syrah vines (vitis vinifera l.) grafted on 110r rootstock to nacl and proline applications

The success of breeding programs aimed stress tolerant plant varieties is limited by the lack ofunderstanding of the molecular basis of salt tolerance. Alternatively to the breeding pr ograms , thetolerance level of plants grown under stress conditions has been increased by using various organicsolutes such as proline. in this study, physiological and biochemical responses of Syrah vines toNaCl stress and exogenously proline application were investigated. Electrolyte leakage (EL), shootgrowth rate (SBO) chlorophyll, proline, malondialdehyde (MDA) , and some of the antioxidantenzyme activities including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), andpolyphenol oxidase (PPO) were measured. Depended upon NaCl levels , an increase in electrolyteloss, chlorophyll degradation, proline , and MDA levels were observed. However, with theapplication of proline, these parameters were ameliorated in part.Proline concentrations used inthis study were moderately effective on antioxidant enzyme system of Syrah grape vines grownunder NaCl stress conditions.Our results may imply that the mechanism controlled by prolinemay involve effects on membrane phase c hanges, lipid peroxidation, and antioxidant enzymesystems.

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Arnon, D.T. 1949. Copper enzymes in insolated chloroplast polyphenoloxidase in Beta vulgaris. Plant Physiology, 23: 1 -15.
Badiani, M., De Biasi, M.G.D. ve Felici, M. 1990. Soluble peroxidase from winter wheat seedlings with phenoloxidase - like activity. Plant Physiology, 93: 489-494.
Badiani, M., Paolacci, A.R., D Annibale, A. ve Sermanni, G.G. 1993. Antioxidants and photosynthesis in the leaves of Triticum durum L. seedlings acclimated to low, non-chilling temperature. Journal of Plant Physiology, 142:18 24.
Bates, L.S., Waldren, R.P. ve Teare, I.D. 1973. Rapid determination of free proline for water - stress studies. Plant Soil, 39: 205 207.
Bohnert, H. J. Ve Shen, B. 1999. Transformation and compatible solutes.Scientia Horticulturae , 78 : 237260.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Analytical Biochemistry, 72:248 -254. Bray, E.A. 1997. Plant responses to wa ter deficit. Trends in Plant Science , 2: 48 - 54.
Chance, B. ve Maehly, A.C. 1955. Assay of catalase and peroxidases. Methods Enzymology, 2: 764-775.
Chowdhury, S.R. ve Shoudhuri, M.A. 1985. Hydrogen peroxide metabolism as an index of water stress tolerance in Jute. Physiologia Plantarum, 65: 476- 480.
Çelik, H., Ağaoglu, Y.S., Fidan, Y., Marasalı, B. ve Söylemezoğlu, G. 1998. Genel Bağcılık. Sun Fidan A.Ş. Mesleki Kitaplar Serisi: 1, Ankara.
Delauney, A. J. ve Verma, D. P. S. 1993. Proline biosynthesis and osmoregulation in plants. The Plant Journal, 4: 215 223.
Dhindsa, R.S., Plumb- Dhindsa, P. ve Thorpe, T.A. 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32: 93 101.
Downtown, W.J.S. 1977. Photosynthesis in salt- stressed grapevines. Australian Journal of Plant Physiology, 4: 183 - 192.
Ehsanpour, AA., Fatahian, N. 2003. Effects of salt and proline on Medicago sativa callus. Plant Cell Tissue and Organ Culture , 73: 53 56.
Elstner, E.F. 1982. Oxygen activation and toxicity. Annual Review of Plant Physiology, 33: 73 96.
Fisarakis, I., Chartzoulakis, K., Stavrakas, D. 2001. Response of Sultana vines ( V. vinifera L.) on six rootstocks to NaCl salinity exposure and recovery. Agricultural Water Management, 51: 13 -27.
Gadallah, M.A.A. 1995. Effects of waterlogging and kinetin on the stability of leaf membranes, leaf osmotic potentia l, soluble carbon and nitrogen compounds and chlorophyll content of Ricinus plants. Phyton, 35: 199- 208.
Gadallah, M.A.A. 1999. Effects of proline glycine betaine on Vicia faba responses to salt stress. Biologia Plantarum , 42(2): 249-257.
Gutteridge, J.M. 1977. The effects of calcium on phospholipid peroxidation. Biochemical and Biophysical Research Communications, 74: 529-37.
Heath, R.L. ve Packer, L. 1968. Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125: 189-198.
Hideg, E. 1997. Free radical production in photosynthesis under stress conditions. In: Pessarakli, M., (ed.) Handbook of Photosynthesis. Marcel Decker, New York, pp. 911 930.
Hoagland D.R. ve Arnon, D.I. 1950. The water - culture method for growing plants without soil. Circular 347. Agricultural Experiment Station, University of California, Berkeley.
Kaya, C., Tuna, A.L., Ashraf, M. ve Altunlu, H. 2007. Improved salt tole rance of melon (Cucumis melo L.) by the addition of proline and potassium nitrate. Environmental and Experimental Botany, 60: 397- 403.
Kohl, D.H., Keennelly, E.H., Zhu, Y., Schubert, K.R. ve Shearer, G.1991. Proline accumulation, nitrogenase (C 2H 2reducing) activity and activities of enzymes related to proline metabolism in drought stressed soybean nodules. Journal of Experimental Botany, 42: 831 837.
Lin, C.C., Hsu, Y.T. ve Kao, C.H. 2002. The effect of NaCl on Proline accumulation in rice leaves. Plant Growth Regulation, 36(3): 275 278.
McCarthy, M.G., Jones, L.D. ve Due, G. 1992. Irrigation, principles and practices. In: Coombe, B.G.,Dry, P.R. (Eds.), Viticulture, vol. II. Practices, Adelaide, Australia, pp. 104- 128.
Ozden, M., D emirel, U. ve Kahraman, A. 2009. Effects of proline on antioxidant system in leaves of grapevine (Vitis vinifera L.) exposed to oxidative stress by H2 O2 . Scientia Horticulturae , 119: 163 168.
Sairam, R.K., Srivastava, G.C., Agarwal, S. ve Meena, R.C . 2005. Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes. Biological Plantarum, 49(1): 85 91.
SAS, 1995. SAS/STAT Users Guide. Version 6.12. SAS Institute, Cary, North Carolina.
Scandolios, J.G. 1993. Oxygen stress and superoxide dismutase. Plant Physiology, 101: 7- 12.
Walker, R.R., Torokfaly, E., Scott, N.S. ve Kriedemann, P.E. 1981. An analysis of photosynthetic response to salt treatment in Vitis vinifera L. Australian Journal of Plant Physiology, 8: 359- 374.
W illiams, L.R. ve Matthews, M.A. 1990. Grapevine. Irrigation of Agricultural Crops. Agr. Monog. No 30, ASA - - SSSA, Madison, WI 53711, USA, pp. 1019-1055.
Winston, G.W. 1990. Physiological basis for free radical formation in cell: Production and defenses. In Stress Responses in plants: Adaptation and Acclimation Mechanisms. Edited by Alscher, R.G. and Cumming, J.R. pp,57-86. Wiley- Liss, Inc., New York.
Woodward, A.J. ve Bennett, I.J. 2005. The effect of sa lt stress and abscisic on proline production, chlorophyll content and growth of in vitro propagated shoots of Eucalyptus camaldulensis. Plant Cell Tissue and Organ Culture , 82: 189200.
Zauberman G, Ronen R, Akerman M, Weksler A., Rot, I. ve Fuchs Y . 1991.Postharvest retention of the red colour of litchi fruit pericarp. Scientia Horticulturae, 47: 89- 97.