İneklerde Tohumlama Sonrası Uygulanan Lesirelin Asetat (GnRH Analogu)'ın Gebelik Oranları Üzerine Etkisi

Bu çalışmada, ineklere suni tohumlama sonrası farklı yollardan lesirelin asetat enjeksiyonlarının gebelik oranlarıüzerindeki etkisi araştırıldı. Materyal olarak postpartum 50-120. günler arasında kendiliğinden östrüse gelen, düzenliaralıklarla seksüel siklus gösteren ve bu süreçte herhangi bir klinik bozukluk göstermeyen farklı ırk ve yaştan 180 inekkullanıldı. Çalışmada kullanılan inekler rastgele 4 gruba ayrıldı. A grubunda bulunan (n= 50) hayvanlara suni tohumlamadanhemen sonra 50 μg lesirelin asetat, B grubu (n=40), hayvanlara 2 ml serum fizyolojik üst epidural boşluğa uygulandı. C grubu(n=50), hayvanlara suni tohumlamadan hemen sonra kas içi 50 μg lesirelin asetat, D grubuna (n=40), 2 ml serum fizyolojikgene kas içi olarak enjekte edildi. Lesirelin asetatın üst epidural boşluğa uygulandığı A ve B grubunda hayvanların gebelikoranları (sırasıyla % 64, % 45) arasında farklılığın istatistiki açıdan anlamlı olduğu (P0.05) belirlendi.Ayrıca, üst epidural ve kas içi GnRH uygulanan gruplar arasında da istatistiki bir farkın (P>0.05) olmadığı tespit edildi. Sonuçolarak, yapılan çalışmada kendiliğinden östrüse gelen ineklere tohumlama ile birlikte epidural yolla uygulanan GnRHenjeksiyonlarının gebelik oranlarını artırmada faydalı olacağı kanaatine varıldı.

Effect on Pregnancy Rates of Injected Lecirelin Acetate (GnRH analogue) after Insemination in the Cows

In the present study the effects of lecirelin acetate injections applied in different ways after artificial insemination on the pregnancy rate of cows were investigated. As a material, 180 cows showing the spontaneous oestrus between 50- 120 days after parturation and having the sexual cycle at regular intervals and showing no clinical disorders during the treatment process were used. The cows were randomly divided into 4 groups as A, B, C and D. In the group A (n = 50) 50 μg lecirelin acetate was injected to the sacrococcygeal epidural space of the animals immediately after the artificial insemination . The animals in the group B (n = 40) were injected with 2 ml physiological saline solution by the same route applied to the animals in the group A. The animals in the group C (n = 50) were injected with 50 μg lecirelin acetate intramuscularly immediately after the artificial insemination. The animals in the group D (n = 40) were injected with 2 ml physiological saline solution by the same route applied to the animals in the group C. Statistically significant in difference (P 0.05) in the pregnancy rates of the groups C and D (60% and 52,5%, respectively). In addition, no significant difference (P>0.05) was observed between the group A (applied epidural GnRH) and C (applied intramuscular GnRH). As a result, it was concluded that either intramuscular or epidural administration of Lecirelin Acetate to cows showing spontaneous oestrus can be used after artificial insemination to increase the pregnancy rates.

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Anjum IA, Usmani RH, Tunio MT, Abro SH, 2009: Improvement of conception rate in crossbred cattle by using GnRH analogue therapy. Pakistan Vet. J, 29, 93-94.
Annalisa R, Debora C, Maddalena M, Giuseppe M, Massimo S, Luigi SR, 2011: Epidural vs intramuscular administration of lecirelin, a GnRH analogue, for the resolution of follicular cysts in dairy cows. Anim Reprod Sci, 126, 19–22.
Bas S, Pinto CG, Day ML, Schuenemann GM, 2012: Effect of intrauterine administration of gonadotropin releasing hormone on serum LH concentrations in lactating dairy cows. Theriogenology, 78, 1390-1397.
Chenault JR, 1990: Effect of fertirelin acetate or buserelin on conception rate at first or second insemination in lactating dairy cows. J Dairy Sci, 73, 633–638.
Dolan S, Evans NP, Richter TA, Nolan AM, 2003: Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor in sheep spinal cord. Neurosci Lett, 346, 120–122.
Espey LL, 1978: Ovarian contractility and its relationship to ovulation: a review. Biol Reprod, 19, 540–551.
Gajewski Z, Thun R, Faundez R, Pawlinski B, 2006: The influence of α-adrenergic receptors stimulator and blockers and beta-blocker on the ovary and endocrinological activity in heifers during ovulation. J Physiol Pharmacol, 57(8), 173–188.
Gumen A, Keskin A, Yilmazbas-Mecitoglu G, Karakaya E, Cevik S, Balci F, 2011: Effects of GnRH, PGF2α and oxytocin treatmentson conception rate at the time of artificial inseminationin lactating dairy cows. Czech J Anim Sci, 56(6), 279–283.
Hayran M, Özdemir O, 1996: Bilgisayar, istatistik ve tıp. 2. Baskı: Medikomat hekimler yayın birliği, Ankara, Türkiye.
Herbert CA, Trigg TE, 2005: Application of GnRH in the control and management of fertility in female animals. Anim Reprod Sci, 88, 141-153.
Kaçar C, Yıldız S, Pancarcı ŞM, Kaya M, Güngör Ö, Arı UÇ, 2007: Effect of administration of lesireline five days after artificial ınsemination on pregnancy rates and luteal function in cows. Kafkas Üniv Vet Fak Derg, 13(2), 139-142.
Kaim M, Bloch A, Wolfenson D, Braw-Tal R, Rosenberg M, Voet H, Folman Y, 2003: Effects of GnRH administered to cows at the onset of estrus on timing ofovulation, endocrine responses, and conception. J Dairy Sci, 86, 2012–2021.
Lee CN, Critser JK, Ax RL, Folman Y, 1985: Changes of luteinizing hormone and progesterone for dairy cows after gonadotropin-releasing hormone at first postpartum breeding. J Dairy Sci, 68, 1463–1470.
Lee CN, Maurice E, Ax RL, Pennington JA, Hoffman WF, Brown MD, 1983: Efficacy of gonadotropinreleasing hormone administered at the time of artificial insemination of heifers and postpartum and repeat breeder dairy cows. American J Vet Res, 44, 2160– 2163.
Lopez-Gatius F, Santolaria P, Martino A, Deletang F, Rensis FD, 2006: The effects of GnRH treatment at the time of AI and 12 days later on reproductive performance of high producing dairy cows during the warm seasonin northeastern Spain. Theriogenology, 65, 820–830.
Martin R, Schmausser M, Seidl S, Schmauder S, Mansfeld R, 2005: Use of Lecirelin to improve insemination efficiency of dairy cattle. Praktısche Tıerarzt, 86(12), 914-919.
Mee MO, Stevenson JS, Scoby RK, 1990: Influence of gonadotropin-releasing hormone and timing of insemination relative to estrus on pregnancy rates of dairy cattle at first service. J Dairy Sci, 73, 1500- 1507.
Millar RP, 2005: GnRHs and GnRH receptors. Anim Reprod Sci, 88, 5-28.
Momcilovic D, Archbald LF, Walters A, Tran T, Kelbert D, Risco C, Thatcher WW, 1998: Reproductive performance of lactating dairy cows treated with gonadotrophin-releasing hormone (GnRH) and/or prostaglandin F2α (PGF2α) for synchronization of estrus and ovulation. Theriogenology, 50(7), 1131- 1139.
Nakao T, Narita S, Tanaka K, Hara, H, Shirakawa J, Noshiro H, Saga N, Tsunoda N, Kawata K, 1983: Improvement of first-service pregnancy rate in cows with gonadotropin-releasing hormone analog. Theriogenology, 20, 111–119.
Padula AM, 2005: GnRH analogues-agonists antantagonists. Anim Reprod Sci 88: 115-126.
Perry GA, Perry BL, 2009: GnRH treatment at artificial insemination in beef cattle fails to increase plasma progesterone concentrations or pregnancy rates. Theriogenology, 71, 775-779
Rizzo A, Minoia G, Trisolini C, Mutinati M, Spedicato M, Manca R, Sciorsci R, 2009: Renin and ovarian vascularization in cows with follicular cysts after epidural administration of a GnRH analogue. Anim Reprod Sci, 116, 226-232.
Rizzo A, Spedicato M, Mutinati M, Minoia G, Pantaleo, M, Sciorsci RL, 2011: In vivoand in vitrostudies of the role of the adrenergic system and follicular wall contractility in the pathogenesis and resolution of bovine follicular cysts. Theriogenology. 76, 1526- 1531.
Robbe D, D’Ottavio M, Sciorsci RL, 2002: Terapia della cisti follicolari della bovina. Obiettivi e Documenti Veterinari (O. D. V), 7/8, 19–24.
Scheiber MD, Liu JH, 2014: The use of Gonadotropin- Releasing Hormone to induce ovulation. https://www.glowm.com/.../cd/.../v5c071.html, Erişim tarihi; 30.10.2014.
Schneider F, Tomek W, Gründker C, 2006: Gonadotropinreleasing hormone (GnRH) and its natural analogues: A review. Theriogenology, 66, 691-709.
Singh R, Graves ML, Roskelley CD, Giritharan G, Rajamahendran R, 2008: Gonadotropin releasing hormone receptor gene and protein expression and immunohistochemical localization in bovine uterus and oviducts. Dom Anim Endocrin, 34, 319-326.
Stevenson JS, Schmidt MK, Call EP, 1984: Gonadotropinreleasing hormone and conception of Holsteins. J Dairy Sci, 67, 140–145.
Şekerden Ö, Uraz H, Söğüt Ö, 2009: Kilis ineklerinde kan progesteron testi kullanılarak erken gebelik teşhis imkanları. Hayvansal Üretim, 50(2), 29-32.
Üstün Z, 2011: Sıcak stresi altındaki süt ineklerinde tohumlama protokolü sonrası epidural GnRH uygulamasının gebelik oranı üzerine etkisi. Yüksek lisans tezi, Adnan Menderes Üniversitesi Sağlık Bilimleri Enstitüsü, Aydın.