Muzaffer Aydın KETANİ, Filiz ÖZCAN, Neval Berrin ARSERİM

Identification of Vibrio parahaemolyticus by PCR Method in Fish and WaterSamples from Tigris River, Turkey

Climate change has caused increasing Vibrio parahaemolyticus infections in areas unaffected by this pathogen.Water temperature is an important ecological factor. It is consequently of high importance to follow V. parahaemolyticus inaquatic environments and foods. The aim of this work was to detect the presence of V. parahaemolyticus in Tigris Riverfrom eight different sites namely Malabadi Bridge, Fiskaya, Ongözlü Bridge, Bağıvar, Pamukçay, Ambarçay, Seyrantepe andSilvan. Total 480 samples of water and fish were used for V. parahaemolyticus detection. Thiosulphatecitrate‐bile saltsucrose (TCBS) Agar was employed for bacteria isolation for 24 h at 37◦C. The dominant uniform bacterial colonies werepurified by streaking onto the TCBS agar plates three times. Identification of this pathogen was made by PCR. A total of 480water and fish samples were analyzed. According to the results, V. parahaemolyticus was detected in 40 (6.6%) fish samplestaken from the Seyrantepe region.

Dicle Nehrindeki Balık ve Su Örneklerinde Vibrio parahaemolyticus’un PCR Yöntemi ile İdentifikasyonu

İklim değişikliğinin bir sonucu olarak artan sayıda Vibrio parahaemolyticus enfeksiyonu tespit edilmiştir. Su sıcaklığıçok güçlü bir ekolojik faktördür. Dolayısıyla su ortamlarında ve su ürünlerinde V. parahaemolyticus'u takip etmek önemarzetmektedir. Bu çalışmanın amacı, Dicle nehrinde bulunan Malabadi Köprüsü, Fiskaya, Ongözlü Köprüsü, Bağıvar,Pamukçay, Ambarçay, Seyrantepe ve Silvan olmak üzere sekiz farklı yerdeki Vibrio parahaemolyticus'un varlığınıaraştırmaktır. Bu amaçla toplam 480 su ve balık numunesi kullanılmıştır. Bakteri izolasyonu için örnekler Tiyosülfatsitrat‐ safra tuzu sukroz (TCBS) Agara ekim yapılmış, 37°C'de 24 saat inkübe edilmiştir ve bakteri kolonileri, TCBS agar plakalarınaüç kez sürme yoluyla saflaştırılmıştır. Patojenin kesin tespiti PCR testi ile gerçekleştirilmiştir. Araştırmanın sonucundaSeyrantepe'deki 40 (6.6%) adet balık örneğinde patojenin varlığı tespit edilmiştir.

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