Aydın ve İzmir İllerindeki Koyun ve Keçilerde Pestivirus Enfeksiyonunun Serolojik ve Virolojik Olarak Araştırılması

Bu çalışma ile Aydın ve İzmir illerindeki koyun ve keçilerde pestivirus enfeksiyonunun serolojik ve virolojik olarak araştırılması amaçlanmıştır. Çalışmada 128 adet keçi, 332 adet koyun olmak üzere toplam 460 adet hayvandan serum örnekleri elde edildi. Alınan kan serumu örneklerinde, bovine viral diarrhea virusunun (BVDV) referans suşu NADL kullanılarak virus nötralizasyon testi ile antikor varlığı araştırıldı. Toplam 348 adet (116 adet keçi, 232 adet koyun) hayvandan alınan tam kan örnekleri pestivirus spesifik antijen varlığı yönünden ticari Enzyme Linked Immunosorbent Assay (ELISA) (Serelisa® BVD p80Ag Mono Indirect, Synbiotics, Europe, Lyon, France) ile test edildi. Keçi örneklerinin %41.40’ının (53 /128), koyun örneklerinin ise %47.59’unun (158/332) seropozitif olduğu saptandı. Seropozitif bulunan toplam 211 serum örneğinin %37.44’ünde 1/5, %34.12’sinde 1/10, %14.21’inde 1/20, %5.21’inde 1/40, %5.68’inde 1/80 ve %3.31’inde ise 1/160 olarak SN50 değerleri belirlendi. Test edilen tam kan örneklerinin antijen yönünden negatif olduğu belirlendi. Bu çalışmada, Aydın ve İzmir illerinde örneklenen koyun ve keçilerde viremik hayvan tespit edilmemiş olmasına rağmen, %45.87 (211/460) oranında seropozitiflik saptanması pestivirusların bölgede sirküle olduğunu ve önemli ekonomik kayıplara neden olabileceğini göstermektedir.

Anahtar Kelimeler:

Antijen, Antikor, Keçi, Koyun, Pestivirus

A Serological and Virological Investigation of the Pestivirus Infections in Sheep and Goats in the Aydin and Izmir Provinces

The objective of this study was serological and virological investigation of pestivirus infections in sheep and goats in the Aydin and Izmir provinces. In the study, 460 serum samples were collected from 128 goats and 332 sheep. The presence of antibodies in the blood serum samples was investigated using NADL, the reference strain for bovine viral diarrhea virus (BVDV) in virus neutralization test. In total, 348 whole blood samples collected from 116 goats and 232 sheep were tested for the presence of the pestivirus specific antigens using commercially available enzyme linked immunosorbent assay (ELISA) (Serelisa® BVD p80ag Mono Indirect, Synbiotics, Europe, Lyon, France). 41.40% (53/128) of the goat and 47.59% (158/332) of the sheep samples were found to be seropositive. SN50 values were 1/5 in 37.44%, 1/10 in 34.12%, 1/20 in 14.21%, 1/40 in 5.21%, 1/80 in 5.68% and 1/160 in 3.31% of the 221 seropositive serum samples. The whole blood samples tested were negative for the antigen. Although no viremia was detected in any of the sheep and goat tested in this study, the 45.87% (211/460) rate of seropositivity shows that Pestiviruses may be circulating in the region and may cause significant economic losses.

Keywords:

Pestivirus, Antigen, Antibody, Goat, Sheep.,

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