Pankreas Kanseri Hücrelerinde Tripartite Motif-Containing Protein 3 (TRIM3) Gen Ekspresyonunun Araştırılması

Amaç: Pankreas kanseri, tüm kanserler içinde en kötü prognoza sahip olanlar arasındadır. Tripartite Motif-Containing Protein 3 (TRIM3) geni tümör baskılayıcı bir gen olarak kanser hücrelerinin proliferasyonu, migrasyonu ve invazyonunu kontrol ederek tümör baskılayıcı olarak rol oynamaktadır. Bu çalışmanın amacı, AsPC1, BxPC-3 ve PANC-1 pankreas kanseri hücre hatlarındaki TRIM3 geninin mRNA seviyesindeki ekspresyonunu araştırmaktır. Materyal ve metod: AsPC1, BxPC-3 ve PANC-1 hücre hatları 37˚C’de %5 CO2 içeren ortamda kültüre edildi ve total RNA izolasyonu yapıldı. TRIM3 geni mRNA ekspresyon seviyesi Kantitatif Ters Transkripsiyon PCR (RT-qPCR) metodu ile incelendi. Relatif gen ekspresyon verilerinin analizi 2-∆∆CT metodu kullanılarak yapıldı. Bulgular: Üç hücre hattında da TRIM3'ün mRNA ekspresyon seviyelerinin çok düşük olduğu tespit edildi. İlaveten kat değişimi hesaplandığında hücre hatları arasında istatistiksel fark gözlenmedi. Sonuç: TRIM3 geni karsinogenez sürecinde tümör baskılayıcı gen olarak rol oynamaktadır ve kanser hücrelerinde TRIM3 ekspresyonunun azaldığı gösterilmiştir. Literatürdeki diğer kanser türleri ile uyumlu şekilde pankreas kanseri hücrelerinde TRIM3 mRNA ekspresyonunun çok düşük olduğu tespit edilmiştir. Bu çalışma AsPC1, BxPC-3 ve PANC-1 pankreas kanseri hücre hatları ve TRIM3 arasındaki ilişkiyi araştıran tek çalışma olması sebebiyle bundan sonra yapılacak fonksiyonel çalışmalara ışık tutacaktır.

Anahtar Kelimeler:

Pankreas kanseri, TRIM3, AsPC1, BxPC-3, PANC-1

Investigation of Tripartite Motif-Containing Protein 3 (TRIM3) Gene Expression in Pancreatic Cancer Cells

Background: Pancreatic cancer is among those with the worst prognosis of all cancers. The Tripartite Motif-Containing Protein 3 (TRIM3) gene, as a tumor suppressor gene, plays a role as a tumor suppressor by controlling the proliferation, migration and invasion of cancer cells. The aim of this study was to investigate the expression of TRIM3 gene at the mRNA level in AsPC1, BxPC-3 and PANC-1 pancreatic cancer cell lines. Materials and Methods: AsPC1, BxPC-3 and PANC-1 cell lines were cultured at 37˚C in an environment containing 5% CO2 and total RNA was isolated. TRIM3 gene mRNA expression level was analyzed by Quantitative Reverse Transcription PCR (RT-qPCR) method. Analysis of relative gene expression data was performed using the 2-∆∆CT method. Results: The mRNA expression levels of TRIM3 were found to be very low in all three cell lines. In addition, when fold change was calculated, no difference was observed between cell lines. Conclusions: TRIM3 gene plays a role as a tumor suppressor gene in the process of carcinogenesis and it has been shown that TRIM3 expression is decreased in cancer cells. Consistent with other cancer types in the literature, TRIM3 mRNA expression was found to be very low in pancreatic cancer cells. Since this study is the only study investigating the relationship between AsPC1, BxPC-3 and PANC-1 pancreatic cancer cell lines and TRIM3, it will shed light on future functional studies.

Keywords:

Pancreatic cancer, TRIM3, AsPC1, BxPC-3, PANC-1,

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