Ali AYÇİÇEK, Ahmet KOÇ, Yeşim OYMAK, Bülent ADAR, Süleymen GETER, Abdullah SOLMAZ

Akut lenfoblastik lösemili çocuk hastaların beyin omurilik sıvısı oksidan/antioksidan seviyeleri

Amaç: Akut lenfoblastik lösemili çocuk hastalarda tedavinin beyin omurilik sıvısında BOS antioxidant ve oksidan seviyelerini araştırmak.Materyal ve Metod: 24 15 erkek 9 kız yeni tanı almış hastaların tanı anında, remisyon indüksiyon, güçlendirme, idame ve tedavi sonunda BOS seruloplazmin ferroksidaz aktivitesi, total tiyol, lipid hidroperoksit LOOH , ve total oksidant seviyeleri TOS çalışıldı. Çalışmaya 19 çocuk 10 erkek 9 kız kontrol olarak alındı. Bulgular: İlk tanıda ALL grubunda kontrol grubuna göre sırasıyla, ortalama ferrokisdaz aktivitesi 50.2 ± 10.4 U/L ve 30.3 ± 7.9 U/L , total tiyol 0.34 ± 0.02 µmol/L ve 0.18 ± 0.01 µmol/L , LOOH 6.5 ± 1.9 µmol/L ve 5.3 ± 0.8 µmol/L , ve TOS 9.3 ± 4.3 µmol H O equiv./L ve 6 ± 1.5 H O equiv./L bulundu P < 0.05 . Ferroksidaz aktivitesi tüm tedavi süresince yüksek seyrettiği, total tiyol seviyesinin ise tedavinin ilk 80 haftasında yüksek kaldığı daha sonra azlamaya başladığı, total oxidant seviyenin ise idamenin 7 haftası ile 120 haftasında düşüşler gösterdiği saptandı.Sonuç: ALL'li çocuk hastaların BOS oksidan stresi ve antioksidanları yüksek seviyededir ve tedavi süresince bu yükseklik devam etmektedir

Anahtar Kelimeler:

Akut lenfoblastik lösemi, antioksidanlar, beyin omurilik sıvısı, oksidanlar, oksidan stress

Cerebrospinal fluid oxidant/antioksidant status in children with acute lymphoblastic leukemia

Objective: To assess the effect of leukemia therapy on the cerebrospinal fluid CSF antioxidant and oxidant status in children with acute lymphoblastic leukemia ALL .Material and Method: Twenty-four consecutive children 15 boys and 9 girls newly diagnosed with ALL were enrolled in the study. CSF concentrations of ceruloplasmin ferroxidase activity, total thiol group, lipid hydroperoxide LOOH and total oxidant status TOS were evaluated at diagnosis, remission induction, consolidation, continuation, and the end of the continuation. Nineteen children 10 boys and 9 girls were enrolled as a reference group. Results: The study group at diagnosis had significant higher mean ferroxidase activity 50.2 ± 10.4 U/L and 30.3 ± 7.9 U/L , total thiol 0.34 ± 0.02 µmol/L and 0.18 ± 0.01 µmol/L , LOOH 6.5 ± 1.9 µmol/L and 5.3 ± 0.8 µmol/L , and TOS 9.3 ± 4.3 µmol H O equiv./L and 6 ± 1.5 H O equiv./L levels compared with the references, respectively P < 0.05 .. Ferroxidase activity remained elevated during the entire treatment course. Total thiol levels stayed elevated from diagnosis to week 80 of ALL treatment and significantly decreased thereafter. LOOH levels were progressively increased to week 80 of the treatment course. TOS levels were progressively decreased two times at continuation week 7 and end of the treatment. Conclusions: Our data indicate that CSF oxidative stress and antioxidants are higher at diagnosis, significant changes to be during therapy, and is already high at the end of therapy in children with ALL

Keywords:

Acute lymphoblastic leukemia, antioxidants, cerebrospinal fluid, oxidants, oxidative stress,

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