Yasin ARSLAN, Şebnem KÖKLÜ, Gökçen YAKUPOĞLU

Karnabahar ve brokoli fidelerine yapılan melatonin uygulamalarının tuz stresi üzerine etkisi

Bu çalışma serin iklim sebze yetiştiriciliğinde önemli bir yere sahip olan karnabahar ve brokoliye dışarıdan yapılan melatonin (MEL) uygulamalarının tuz stresi üzerine olası etkilerini belirleyebilmek amacıyla gerçekleştirilmiştir. Bu sebeple 4-6 gerçek yapraklı karnabahar ve brokoli fidelerine farklı dozlarda MEL (0, 5 ve 10 μM MEL) içeren sulu çözelti topraktan uygulanmıştır. Tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak kurulan denemede stres etkilerini belirleyebilmek amacıyla fidelerde fiziksel (fide ağırlıkları, boyu, çapı ve yaprak alanı) ve biyokimyasal (elektriki iletkenlik, klorofil ve karotenoid içeriği, prolin, melondialdehit ve toplam fenolik madde içeriği) analizler gerçekleştirilmiştir. Analizler sonucunda stres altındaki fidelere dışarıdan yapılan MEL uygulamalarının stresin olumsuz etkilerini azaltarak boy, çap, ağırlık, klorofil, karetenoid, antosiyanin ve fenolik madde içeriğinde iyileşmeler sağladığı gözlemlenmiştir. Sonuç olarak dışarıdan yapılan MEL uygulamaları tuz stresinin olumsuz etkilerini azaltmada etkili olmuş, bununla birlikte karnabaharda 5μM MEL uygulaması, brokoli fidelerinde ise 5μM-10 μM MEL uygulamaları uygun dozlar olarak belirlenmiştir.

Anahtar Kelimeler:

Tuz Stresi, , Melatonin, Karnabahar, Brokkoli

The effect of melatonin treatments on cauliflower and broccoli seedlings on salt stress

This study was carried out to determine the possible effects of exogen melatonin (MEL) treatments on salt stress in cauliflower and broccoli, which have a significant position among cool region vegetables. For that reason, an aqueous solution containing at different concentrations MEL (0, 5, and 10 μM MEL) were applied to the cauliflower and broccoli seedlings with 4-6 true leaves from the soil. In this experiment, which was established with 3 replications according to the randomized plots trial design, physical (seedling weight, height, diameter, and leaf area) and biochemical (electrical conductivity, chlorophyll and carotenoid content, proline, malondialdehyde, and total phenolic substance content) analyzes were carried out to determine the stress effects on the seedlings. As a result of the analyzes, it was observed that exogen MEL treatments to the seedlings under stress reduced the unfavorable effects of stress and provided improvements in height, diameter, weight, chlorophyll, carotenoid, anthocyanin, and phenolic substance content. As a result, exogen MEL treatments were very efficient in reducing the unfavorable effects of salt stress. However, 5μM MEL treatment in cauliflower and 5 and 10 μM MEL tratments in broccoli seedlings were determined as appropriate concentrations.

Keywords:

salt stress, melatonin, cauliflower, broccoli,

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