Yeni İzole Ediilmiş Trichoderma Citrinoviride’den Lipaz Üretiminin Saptanması

Bu çalışmada yeni izole edilmiş bir fungustan lipaz üretimi amaçlanmıştır. Fungus Kocaeli bölgesinden topraktan izole edilmiştir. 18S rRNA analizi sonucu Trichoderma citrinoviride olarak saptanmıştır. Lipaz üretimi için çeşitli parametreler ve besiyeri bileşenleri araştırılmıştır. Sırasıyla, glikoz ve pepton en uygun karbon ve azot kaynağı olarak bulunmuştur. Karbon kaynağı olarak uygun yağı tespit edilmesi için, üretim ortamına çeşitli yağlar eklenmiştir. Zeytinyağının Trichoderma citrinoviride’den lipaz üretimi için en uygun yağ olduğu bulunmuştur. pH 5.5, sıcaklık 30°C ve 4 günlük inkübasyon süresi lipaz üretimi için en uygun inkübasyon koşulları olarak bulunmuştur. Alternative karbon kaynağı olarak melas ortamında üretilen Trichoderma citrinoviride’den lipaz verimi de tespit edilmiştir.

Detection of Lipase Production from Newly Isolated Trichoderma Citrinoviride

The production of lipase is aimed from a new fungal source in this study. Fungus was isolated from soil locality Kocaeli. It was determined as Trichoderma citrinoviride by the analysis of 18S rRNA sequence. Various parameters and media components were investigated for production of lipase. Glucose and peptone were found to be most suitable carbon and nitrogen source, respectively. To determine the suitable oil as carbon source, various oils were added to the production medium. Olive oil was found to be the optimal oil for lipase production from Trichoderma citrinoviride. pH 5.5, temperature 30°C and incubation time for 4 days were found to be optimal incubation conditions for lipase production. We also determined lipase yield from Trichoderma citrinoviride which is produced in molasses medium as an alternative carbon source.

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Hacettepe Journal of Biology and Chemistry-Cover

ISSN: 2687-475X
Yayın Aralığı: Yılda 4 Sayı
Başlangıç: 1972
Yayıncı: Hacettepe Üniversitesi, Fen Fakültesi

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