Cholesterol Adsorption from Artificial Human Plasma with Molecular Imprinted Polymeric Nanostructures
This study reports cholesterol adsorption from artificial human plasma using MIP nanostructures prepared
with different template:monomer ratios. The adsorption capacity of CP is 19.9% and 16.1% higher than those
of C3P and CP3, respectively and adsorption capacity of CP is significantly higher than NIP nanostructures.
All selectivity coefficients and relative selectivity values were higher than 1 for artificial human plasma. Under
optimum conditions, considerably high cholesterol was adsorbed from hypercholesterolemic plasma (95.33 %).
___
- S.N. Hashim, R.I. Boysen, L.J. Schwarz, B. Danylec,
M.T. Hearn, A comparison of covalent and noncovalent
imprinting strategies for the synthesis of
stigmasterol imprinted polymers, J. Chromatogr. A,
1359 (2014) 35-43
- E. Verheyen, J.P. Schillemans, M. Wijk, M.A.
Demeniex, W.E. Hennink, C.F. Nostrum, Challenges
for the effective molecular imprinting of proteins,
Biomaterials, 32 (2011) 3008-3020.
- M. Behbahani, S. Bagheri, M.M. Amini, H.S.
Abandansari, H.R. Moazami, A. Bagheri, Application
of a magnetic molecularly imprinted polymer for
the selective extraction and trace detection of
lamotrigine in urine and plasma samples, J. Sep. Sci.,
37 (2014) 1610-1616.
- C. Lu, H. Li, M. Xu, S. Wang, G. Li, W. Zhong, S.Qin,
Preparation of nicotine-imprinted monolith by insitu
surface imprinting onto internal hole surface of
macroporous silica for selective enrichment and
separation of nicotine in environmental water sample,
Sep. Sci. Technol., 50 (2015) 2124-2133.
- J. Ding, F. Zhang, X. Zhang, L. Wang, C. Wang, Q. Zhao,
Y. Xu, L. Ding, N. Ren, Determination of roxithromycin
from human plasma samples based on magnetic
surface molecularly imprinted polymers followed by
liquid chromatography-tandem mass spectrometer, J.
Chromatogr. B, 1021 (2016) 221-228.
- X. Xu, S. Liang, X. Meng, M. Zhang, Y. Chen, D. Zhao, Y.
Li, A molecularly imprinted polymer for the selective
solid-phase extraction of dimethomorph from ginseng
samples, J. Chromatogr. B, 988 (2015) 182-186.
- X. Kong, R. Gao, X. He, L. Chen, Y. Zhang, Synthesis
and characterization of the core–shell magnetic
molecularly imprinted polymers (Fe3O4@MIPs)
adsorbents for effective extraction and determination
of sulfonamides in the poultry feed, J. Chromatogr. A,
1245 (2012) 8-16.
- M. Andaç, I.Y. Galaev, A. Denizli, Affinity based and
molecularly imprinted cryogels: Applications in
biomacromolecule purification, J. Chromatogr. B,
1021 (2016) 69-80.
- J. Yang, Z. Wang, T. Zhou, X. Song, Q. Liu, Y. Zhang, L.
He, Determination of cyproheptadine in feeds using
molecularly imprinted solid-phase extraction coupled
with HPLC, J. Chromatogr. B, 990 (2015) 39-44.
- C. Hwang, W.C. Lee, Chromatographic characteristics
of cholesterol imprinted polymers prepared by
covalent and non-covalent imprinting methods, J.
Chromatogr. A, 962 (2002) 69-78.
- E. Caro, N. Masque, R.M. Marce, F. Borrull, P.A.G.
Cormack, D.C. Sherington, Non-covalent and semicovalent
molecularly imprinted polymers for selective
on-line solid-phase extraction of 4-nitrophenol from
water samples, J. Chromatog. A, 963 (2002) 169-178.
- F. Lanza, A.J. Hall, B. Sellergen, A. Bereczki, G.
Horvai, S. Bayoudh, P.A.G. Cormack, D.C. Sherington,
Development of semiautomated procedure for the
synthesis and evaluation of molecularly imprinted
polymers applied to the search for functional
polymers for phenytoin and nifedipine, Anal. Chim.
Acta, 435 (2001) 91-106.
- A. Ersöz, A. Denizli, İ. Şener, A. Atılır, S. Diltemiz, R. Say,
Removal of phenolic compounds with nitrophenolimprinted
polymer based on p-p and hydrogenbonding
interactions, Sep. Purif. Technol., 38 (2004)
173-179.
- R. Say, A. Ersöz, İ. Şener, A. Atılır, S. Diltemiz, A.
Denizli, Comparison of adsorption and selectivity
characteristics for 4 nitrophenol imprinted polymers
prepared via bulk and suspension polymerization,
Sep. Sci. Technol., 39 (2004) 3471-3484.
- E. Yılmaz, K. Mosbach, K. Haupt, Influence of
functional and cross-linking monomers and
the amount of template on the performance of
molecularly imprinted polymers in binding assays,
Anal. Commun., 36 (1999) 167-170.
- C.H. Hu, T.C. Chou, Albumin molecularly imprinted
polymer with high template affinity-prepared by
systematic optimization in mixed organic/aqueous
media, Microchem. J., 91 (2009) 53-58.
- X. Sun, J. Wang, Y. Li, J. Yang, J. Jin, S.M. Shah, J.
Chen, Novel dummy molecularly imprinted polymers
for matrix solid-phase dispersion extraction of eight
fluoroquinolones from fish samples, J. Chromatog. A,
1359 (2014) 1-7.
- S. Aşir, D. Sari, A. Derazshamshir, F. Yılmaz, K. Şarkaya,
A. Denizli, Dopamine imprinted monolithic column for
capillary electrochromatography, Electrophoresis, 0
(2017) 1-10.
- A.G. Sarıkaya, B. Osman, T. Çam, A. Denizli, Molecularly
imprinted surface plasmon resonance (SPR) sensor
for uric acid determination, Sens. Actuators B Chem.,
251 (2017) 763-772.
- G. Sener, E. Ozgur, E. Yılmaz, L. Uzun, R. Say, A. Denizli,
Quartz crystal microbalance based nanosensor
for lysozyme detection with lysozyme imprinted
nanoparticles. Biosens. Bioelectron., 26 (2010) 815-
821.
- Y. Saylan, S. Akgönüllü, D. Çimen, A. Derazshamshir,
N. Bereli, F. Yılmaz, A. Denizli, Development of surface
plasmon resonance sensors based on molecularly
imprinted nanofilms for sensitive and selective
detection of pesticides, Sens. Actuators B Chem., 241
(2017) 446-454.
- L.I. Andersson, Molecular imprinting for drug
bioanalysis A review on the application of imprinted
polymers to solid-phase extraction and binding assay,
J. Chromatogr. B, 739 (2000)163-173.
- E. Turiel, A. Martin-Esteban, Molecularly imprinted
polymers for sample preparation: a review, Anal.
Chim. Acta, 66 (2010) 887-899.
- L. Chen, S. Xu, X. Li, Recent advances in molecular
imprinting technology: current status, challenges and
highlighted applications, Chem. Soc. Rev., 40 (2011)
2922-2942.
- A. Aghaei, M.R.M. Hosseini, M. Najafi, A novel
capacitive biosensor for cholesterol assay that
uses an electropolymerized molecularly imprinted
polymer, Electrochimica Acta, 55 (2010) 1503-1508.
- A. Sinha, S. Basiruddin, A. Chakraborty, N.R. Jana,
Cyclodextrin functionalized magnetic mesoporous
silica colloid for cholesterol separation, ACS Appl.
Mater. Interfac., 7 (2015) 1340-1347.
- Y. Su, Y. Tian, R. Yan, C. Wang, F. Niu, Y. Yang, Study on
a novel process for the separation of phospholipids,
triacylglycerol and cholesterol from egg yolk, J. Food
Sci. Technol., 52 (2015) 4586-4592.
- D.N. Clausen, I.M.R. Pires, C.R.T. Tarley, Improved
selective cholesterol adsorption by molecularly
imprinted poly(methacrylic acid)/silica (PMAA–SiO2)
hybrid material synthesized with different molar
ratios, Mat. Sci. Eng. C, 44 (2014) 99-108.
- M.M. Jimenez-Carmona, M.D.L. de Castro, Reverse
micelle formation for acceleration of the supercritical
fluid extraction of cholesterol from food samples,
Anal. Chem., 70 (1998) 2100-2103.
- E.E.G. Rojas, J.S.D. Coimbra, L.A. Minim, Adsorption
of egg yolk plasma cholesterol using a hydrophobic
adsorbent, Eur. Food Res. Technol., 223 (2006) 705-
709.
- A. Zengin, E. Yildirim, U. Tamer, T. Caykara,
Molecularly imprinted superparamagnetic iron oxide
nanoparticles for rapid enrichment and separation of
cholesterol, Analyst, 138 (2013) 7238-7245.
- Y. Tong, H. Guan, S. Wang, J. Xu, J. He, Syntheses of
chitin-based imprinting polymers and their binding
properties for cholesterol, Carbohyd. Res., 346 (2011)
495-500.
- R. Gupta, A. Kumar, Synthesis and characterization
of sol–gel-derived molecular imprinted polymeric
materials for cholesterol recognition, J. Sol-Gel Sci.
Technol., 58 (2011) 182-194.
- R. Say, S. Emir, B. Garipcan, S. Patir, A. Denizli, Novel
methacryloylamidophenylalanine functionalized
porous chelating beads for adsorption of heavy metal
ions, Adv. Polym. Tech., 22 (2003) 355-364.
- T. Inanan, N. Tuzmen, S. Akgöl, A. Denizli, Selective
cholesterol adsorption by molecular imprinted
polymeric nanospheres and application to GIMS, Int.
J. Biol. Macromolec., 92 (2016) 451-460.
- L. Navakova, P. Solich, L. Matysova, J. Sıcha, HPLC
determination of estradiol, its degradation product,
and preservatives in new topical formulation estrogel
HBF, Anal. Bioanal. Chem., 379 (2004) 781-787.
- M.A. Gore, R.N. Karmalkar, M.G. Kulkarni, Enhanced
capacities and selectivities for cholesterol in aqueous
media by molecular imprinting: role of novel crosslinkers,
J. Chromatog. A, 804 (2004) 211-221.
- X. Li, M. Li, J. Li, F. Lei, X. Su, X. Liu, P. Li, X. Tan,
Synthesis and characterization of molecularly
imprinted polymers with modified rosin as a cross
linker and selective SPE-HPLC detection of basic
orange II in foods, Anal. Met., 6 (2014) 6397-6406.
- H.S. Andersson, J.G. Karlsson, S.A. Piletsky, A.C.
Koch-Schmidt, K. Mosbach, I.A. Nicholls, Study of
the nature of recognition in molecularly imprinted
polymers, influence of monomer–template ratio
and sample load on retention and selectivity, J.
Chromatogr. A, 848 (1999) 39-49.
- G.R. Oliveira, A.V. Santos, A.S. Lima, C.M.F. Soares,
M.S. Leite, Neural modelling in adsorption column of
cholesterol-removal efficiency from milk, LWT- Food
Science and Technol., 64 (2015) 632-638.
- D.K. Lee, J. Ahn, H.S. Kwak, Cholesterol removal from
homogenized milk with β-cyclodextrin, J Dairy Sci.,
82 (1999) 2327-2330.
- K. Çaktü, G. Baydemir, B. Ergün, H. Yavuz, Cholesterol
removal from various samples by cholesterolimprinted
monosize microsphere-embedded cryogels,
Artif. Cells Nanomed. Biotechnol., 42 (2014) 365-375.
- H. Yavuz, V. Karakoç, D. Türkmen, R. Say, A. Denizli,
Synthesis of cholesterol imprinted polymeric
particles, Int. J. Biol. Macromol., 41 (2007) 8-15.
- M. Odabaşı, L. Uzun, G. Baydemir, N.H. Aksoy, Ö.
Acet, D. Erdönmez, Cholesterol imprinted composite
membranes for selective cholesterolrecognition
from intestinal mimicking solution, Colloids Surf. B
Biointer., 163 (2018) 266–274.
- H.M.A.M. Dias, F. Berbicz, F. Pedrochi, M.L. Baesso,
G. Matioli, Butter cholesterol removal using different
complexation methods with beta-cyclodextrin, and
the contribution of photoacoustic spectroscopy to
the evaluation of the complex, Food Res. Int., 43
(2010) 1104–1110.
- S.H. Chiu, T.W. Chung, R. Giridhar, W.T. Wu,
Immobilization of b-cyclodextrin in chitosan beads
for separation of cholesterol from egg yolk, Food Res.
Int., 37 (2004) 217-223.
- E. Salehi, S. Afshar, M.Z. Mehrizi, A. Chehrei, M. Asadi,
Direct reduction of blood serum cholesterol using
Thymus vulgaris L.: Preliminary biosorption study,
Process Bichem., accepted manuscript
- I. Polyakova, L. Borovikova, A. Osipenko, E. Vlasova,
B. Volchek, O. Pisarev, Surface molecularly imprinted
organic-inorganic polymers having affinity sites for
cholesterol, React. Funct. Polym., 109 (2016) 88-98.
- N. Bereli, G. Şener, H. Yavuz, A. Denizli. Oriented
immobilized anti-LDL antibody carrying
poly(hydroxyethyl methacrylate) cryogel for
cholesterol removal from human plasma, Mater. Sci.
Eng. C, 31 (2011) 1078–1083.
- Yun S., J.H. Zhang, D.Shi, M. Jiang, Y.X. Zhu, S.R.
Mei, Y.K. Zhou, K. Dai, B. Lu, Selective solid-phase
extraction of cholesterol using molecularly imprinted
polymers and its application in different biological
samples, J. Pharm. Biomed. Anal., 42 (2006) 549–
555.