Nilüfer AKSÖZ, Demet ERDÖNMEZ, Kübra ERKAN TÜRKMEN

İç Anadolu’daki Tuz Gölü’nden İzole Edilen Çeşitli Halofilik Bakterilerdeki Quorum Sensing Sistemine Yeni Bir Yaklaşım

Quorum sensing (qs), bir mikroorganizmanın yaşadığı ortamdaki diğer popülasyon üyeleri ile ortak davranışları geliştirmek için kullandığı bir sistemdir. İçeriğindeki tuz yoğunluğundan dolayı ekstrem ortamlardan sayılan Tuz Gölü, bünyesinde barındırdığı planktonik canlılar ve bakteriler yönünden zengindir. Özellikle tuz gölü uygun üreme koşullarını sağladığı halofilik bakteriler için çok önemli bir alandır. Tuz Gölü›nden izole edilen Halofilik karakterli 20 bakteri türünün qs sinyal moleküllerinden N-asil homoserin lakton (AHL) türevlerini üretebildikleri saptanmıştır. Bu bakteriyel sinyal moleküllerini tespit etmek için biyosensör bakteriler Chromobacterium violaceum cv026 ve Agrobacterium tumefaciens A136 kullanılarak ince tabaka kromatografisinden yararlanılmıştır. Sinyal molekülü üretebilen suşların 16sRNA ile genomik analizleri gerçekleştirilmiştir. Halobacterium salinarium, Salicola türleri, Halovibrio ve Halomonas türlerinin Tuz Gölü içerisinde yaygın olduğu görüldü. Ayrıca bu suşların C6-AHL ve C8-AHL sinyal moleküllerini üretebildikleri ve biyofilm oluşturabildikleri gözlenmiştir.

A New Approach for Quorum Sensing System in Several Halophilic Bacteria Isolated from Salt Lake in Central Anatolia

Quorum sensing (qs) is the system that microorganisms use to improve common behavior with another population member in their environment. Salt Lake in Turkey that is considered as an extreme environment due to its salt concentration, teems in plankton and bacteria. Especially, it is an important field for halophilic bacteria because it provides suitable growth conditions. In this study, it was determined that 20 halophilic bacteria strain isolated from Salt Lake could produce qs signal molecules which are N-acyl homoserine lactone (HSL) derivatives. For detection of these bacterial signal molecules, thin layer chromatography was utilized by using Chromobacterium violaceum cv026 and Agrobacterium tumefaciens A136 that are biosensor bacteria strains. Genomic analysis of strains that produce signal molecules was carried out via 16s rRNA. Halobacterium salinarium, Salicola sp., Halovibrio, and Halomonas were found to be common in Salt Lake. It was also observed that these strains could produce C6-HSL and C8-HSL signaling molecules and produce biofilms.

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