Yağ Çıkarma İşlemi Sonrasında Atık Materyal Olarak Ortaya Çıkan Çörek Otu Posasının Anti-oksidan, Anti-proliferatif ve Antianjiyojenik Özellikleri

Bu çalışmanın amacı, yağ çıkarma işlemi sonrasında atık materyal olarak ortaya çıkan çörek otu (Nigella sativa L.-NS) posasının anti-oksidan, anti-metastatik ve anti-anjiyojenik özelliklerinden yola çıkarak biyolojik etkinliklerini incelemektir. NS posasından çıkarılan özütün UV-görünür bölge (UV-vis), Fourier Dönüşümlü Kızılötesi (FTIR) ve gaz kromatografisi-kütle spektrometresi (GC-MS) ölçümleriyle karakteristiksel özellikleri incelendikten sonra 2,2-difenil- 1-pikrilhidrazil (DPPH) ve Trolox eşdeğer antioksidan kapasitesi (TEAC) yöntemleri kullanılarak anti-oksidatif kapasitesi tayin edilmiştir. Posa özütünün anti-proliferatif etkileri, etkilerin hücre tipine özgü olup olmadığını incelemek amacıyla hem kanser hem de kanser olmayan hücreler üzerinde test edilmiştir. Anti-anjiyojenik testler civciv korioallantoik membran testi (CAM) aracılığıyla gerçekleştirilmiştir. Sonuçlar NS posa ekstratının radikal süpürücü etkilerini halen muhafaza ettiğini ve normal hücreler ile karşılaştırıldığında kanser hücre proliferasyonunu daha etkin bir biçimde düşürdüğünü göstermiştir. Ayrıca CAM testleri NS posa özütünün VEGF uyarımlı vaskülarizasyon ve gelişimini etkin bir biçimde sınırladığını göstermiştir. NS posa özütü metastatik hücre topluluklarının baskılanmasında ve kanser başta olmak üzere patolojik anjiyogenez ile seyreden hastalıkların tedavisinde faydalı sonuçlar üretebilir. Bu çalışma bitkisel kaynaklı atık maddelerin birçok alanda çok çeşitli amaçlar doğrultusunda kullanılmak üzere geri dönüştürülebileceğine dikkat çekmektedir.

Antioxidative, Antiproliferative and Antiangiogenic Activities of Nigella sativa L. pulp, a Waste Material Remaining from Oil Production

The aim of this study was to investigate the biological activities such as antioxidative, antimetastatic and antiangiogenic properties of the Nigella sativa L. (NS) pulp, which is a waste material of the oil production process. Following the investigation of the characteristic properties of NS pulp extract with UV-visible (UVvis), fourier-transform infrared (FTIR) and gas chromatography-mass spectroscopy (GC-MS) measurements, its antioxidative capacity was determined using 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and Trolox equivalent antioxidant capacity (TEAC) assays. Antiproliferative effects of the pulp extract were tested on both cancerous and non-cancerous cells to examine whether the effects are specific to cell types. Antiangiogenic tests were performed via chick chorioallantoic membrane (CAM) assay. The results showed that the NS pulp extract still maintained its radical scavenging effects and reduced the proliferation of cancer cells more efficiently compared to non-cancerous cells. Furthermore, CAM assays demonstrated that the pulp extract effectively limited vascular endothelial growth factor (VEGF)-stimulated vascularization and development. NS pulp might be used for the suppression of the metastatic cell populations and for the treatment of the diseases particularly like cancer, which progress with pathological angiogenesis. This study highlights that such waste materials from plants can be recycled with various uses in many fields.

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